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Q0GY40 (KBX3_HADGE) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 25. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hge-scorpine
Alternative name(s):
Hg-scorpine-like 1
Short name=HgeScplp1
Short name=Hgscplike1

Cleaved into the following chain:

  1. Hge36
OrganismHadrurus gertschi (Scorpion)
Taxonomic identifier380989 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesIuridaIuroideaHadrurus

Protein attributes

Sequence length95 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hge-scorpine has antibacterial activity against B.subtilis, but not against S.aureus. Also has hemolytic and cytolytic activities. Ref.3

Hge36 peptide blocks Kv1.1/KCNA1 (IC50=185 nM) potassium channels. Shows a weak hemolytic activity. Ref.3

Subcellular location

Secreted.

Tissue specificity

Expressed by the venom gland.

Miscellaneous

The C-terminus (AA 52-95) blocks Kv1.1/KCNA1, Kv1.2/KCNA2, and Kv1.3/KCNA2 potassium channels, showing a potential important role of AA 48-51.

Hge36 peptide does not block Kv1.2/KCNA2 and Kv1.3/KCNA3 (Ref.3).

Sequence similarities

Belongs to the long chain scorpion toxin family. Class 3 subfamily.

Mass spectrometry

Molecular mass is 8370.0 Da from positions 20 - 95. Determined by ESI. Ref.2

Molecular mass is 8370.0 Da from positions 20 - 95. Determined by MALDI. Ref.3

Molecular mass is 5294.95 Da from positions 48 - 95. Determined by MALDI. Ref.3

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Ref.2 Ref.3
Chain20 – 9576Hge-scorpine
PRO_0000274682
Chain48 – 9548Hge36
PRO_0000356887

Amino acid modifications

Disulfide bond58 ↔ 81 By similarity
Disulfide bond68 ↔ 86 By similarity
Disulfide bond72 ↔ 88 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0GY40 [UniParc].

Last modified October 3, 2006. Version 1.
Checksum: EA797C1F58CF6C3C

FASTA9510,412
        10         20         30         40         50         60 
MNTKLTVLCF LGIVTIVSCG WMSEKKVQGI LDKKLPEGII RNAAKAIVHK MAKNQFGCFA 

        70         80         90 
NVDVKGDCKR HCKAEDKEGI CHGTKCKCGV PISYL 

« Hide

References

« Hide 'large scale' references
[1]"Transcriptome analysis of the venom gland of the Mexican scorpion Hadrurus gertschi (Arachnida: Scorpiones)."
Schwartz E.F., Diego-Garcia E., Rodriguez de la Vega R.C., Possani L.D.
BMC Genomics 8:119-119(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Venom gland.
[2]"Wide phylogenetic distribution of scorpine and long-chain beta-KTx-like peptides in scorpion venoms: identification of 'orphan' components."
Diego-Garcia E., Schwartz E.F., D'Suze G., Gonzalez S.A., Batista C.V., Garcia B.I., Rodriguez de la Vega R.C., Possani L.D.
Peptides 28:31-37(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 20-64, MASS SPECTROMETRY.
Tissue: Venom and Venom gland.
[3]"Cytolytic and K+ channel blocking activities of beta-KTx and scorpine-like peptides purified from scorpion venoms."
Diego-Garcia E., Abdel-Mottaleb Y., Schwartz E.F., Rodriguez de la Vega R.C., Tytgat J., Possani L.D.
Cell. Mol. Life Sci. 65:187-200(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 20-64, MASS SPECTROMETRY, FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ465351 mRNA. Translation: ABE98267.1.
EF613116 Genomic DNA. Translation: ABU94956.1.
EL698908 mRNA. No translation available.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameKBX3_HADGE
AccessionPrimary (citable) accession number: Q0GY40
Secondary accession number(s): A8SDT6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: October 3, 2006
Last modified: October 16, 2013
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Scorpion potassium channel toxins

Nomenclature of scorpion potassium channel toxins and list of entries