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Q0GNC1

- INF2_MOUSE

UniProt

Q0GNC1 - INF2_MOUSE

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Protein
Inverted formin-2
Gene
Inf2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Severs actin filaments and accelerates their polymerization and depolymerization.1 Publication

Enzyme regulationi

Phosphate inhibits both the depolymerization and severing activities.1 Publication

GO - Biological processi

  1. actin cytoskeleton organization Source: InterPro
  2. regulation of cellular component size Source: MGI
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Inverted formin-2
Gene namesi
Name:Inf2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:1917685. Inf2.

Subcellular locationi

Cytoplasmperinuclear region By similarity

GO - Cellular componenti

  1. perinuclear region of cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi1009 – 10091L → A: Strongly inhibits depolymerization; when associated with A-1010 and A-1019. 1 Publication
Mutagenesisi1010 – 10101L → A: Strongly inhibits depolymerization; when associated with A-1009 and A-1019. 1 Publication
Mutagenesisi1019 – 10191L → A: Strongly inhibits depolymerization; when associated with A-1009 and A-1010. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 12731272Inverted formin-2
PRO_0000259890Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine By similarity
Modified residuei1172 – 11721Phosphoserine1 Publication
Modified residuei1174 – 11741Phosphoserine1 Publication
Modified residuei1203 – 12031Phosphothreonine By similarity
Modified residuei1216 – 12161Phosphoserine By similarity
Modified residuei1230 – 12301Phosphothreonine By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ0GNC1.
PaxDbiQ0GNC1.
PRIDEiQ0GNC1.

PTM databases

PhosphoSiteiQ0GNC1.

Expressioni

Gene expression databases

GenevestigatoriQ0GNC1.

Interactioni

Subunit structurei

Interacts with profilin and actin at the FH1 and FH2 domains respectively.1 Publication

Protein-protein interaction databases

BioGridi214049. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ0GNC1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 330329GBD/FH3
Add
BLAST
Domaini421 – 564144FH1
Add
BLAST
Domaini589 – 979391FH2
Add
BLAST
Domaini1007 – 102216WH2
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili907 – 98478 Reviewed prediction
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi421 – 563143Pro-rich
Add
BLAST

Domaini

The WH2 domain acts as the DAD (diaphanous autoregulatory) domain and binds to actin monomers.1 Publication
Regulated by autoinhibition due to intramolecular GBD-DAD binding.1 Publication
The severing activity is dependent on covalent attachment of the FH2 domain to the C-terminus.1 Publication

Sequence similaritiesi

Belongs to the formin homology family.
Contains 1 WH2 domain.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG149898.
HOGENOMiHOG000113072.
HOVERGENiHBG081794.
InParanoidiQ0GNC1.
PhylomeDBiQ0GNC1.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR015425. FH2_Formin.
IPR010472. FH3_dom.
IPR010473. GTPase-bd.
IPR014768. GTPase-bd/formin_homology_3.
IPR027649. Inf2.
IPR003124. WH2_dom.
[Graphical view]
PANTHERiPTHR23213:SF5. PTHR23213:SF5. 1 hit.
PfamiPF06367. Drf_FH3. 1 hit.
PF06371. Drf_GBD. 1 hit.
PF02181. FH2. 1 hit.
PF02205. WH2. 1 hit.
[Graphical view]
SMARTiSM00498. FH2. 1 hit.
SM00246. WH2. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS51444. FH2. 1 hit.
PS51232. GBD_FH3. 1 hit.
PS51082. WH2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q0GNC1-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSVKEGAQRK WAALKEKLGP QDSDPTEANL ESAEPELCIR LLQMPSVVNY     50
SGLRKRLESS DGGWMVQFLE QSGLDLLLEA LARLSGRGVA RISDALLQLT 100
CISCVRAVMN SQQGIEYILS NQGYVRQLSQ ALDTSNVMVK KQVFELLAAL 150
CIYSPEGHAL TLDALDHYKM VCSQQYRFSV IMSELSDSDN VPYVVTLLSV 200
INAIILGPED LRSRAQLRSE FIGLQLLDIL TRLRDLEDAD LLIQLEAFEE 250
AKAEDEEELQ RISDGINMNS HQEVFASLFH KVSCSPASAQ LLSVLQGLMH 300
LEPAGRSGQL LWEALENLVN RAVLLASDAQ ACTLEEVVER LLSIKGRPRP 350
SPLDKAHKSV QTNSVQNQGS SSQNTTTPTT KVEGQQPVVA SPCQHVGSIQ 400
SSSVDIAPQP VALEQCITAL PLPTPPLSSS TPVLPPTPPP LPGPGATSPL 450
PPPPPPLPPP LPGSGTTSPP PPPPPPPPLP PPLPGSGTIS PPPPPPPPPL 500
PGTGAVSPPP PPPLPSLPDS HKTQPPPPPP PPLPGMCPVP PPPPLPRAGQ 550
IPPPPPLPGF SVPSMMGGVE EIIVAQVDHS LGSAWVPSHR RVNPPTLRMK 600
KLNWQKLPSN VARERNSMWA TLGSPCTAAV EPDFSSIEQL FSFPTAKPKE 650
PSAAPARKEP KEVTFLDSKK SLNLNIFLKQ FKCSNEEVTS MIQAGDTSKF 700
DVEVLKQLLK LLPEKHEIEN LRAFTEERAK LSNADQFYVL LLDIPCYPLR 750
VECMMLCEGT AIVLDMVRPK AQLVLTACES LLTSQRLPVF CQLILKIGNF 800
LNYGSHTGDA DGFKISTLLK LTETKSQQSR VTLLHHVLEE VEKSHPDLLQ 850
LSRDLEPPSQ AAGINVEIIH SEASANLKKL LEAERKVSAS IPEVQKQYAE 900
RLQASIEASQ ELDKVFDAIE QKKLELADYL CEDPQQLSLE DTFSTMKTFR 950
DLFTRALKEN KDRKEQMAKA ERRKQQLAEE EARRPRDEDG KPIRKGPGKQ 1000
EEVCVIDALL ADIRKGFQLR KTARGRGDTE ASGRVAPTDP PKATEPATAS 1050
NPTQGTNHPA SEPLDTTAAD EPQGWDLVDA VTPSPQPSKE EDGPPALERR 1100
SSWYVDAIDF LDPEDTPDAQ PSEGVWPVTL GDGQALNPLE FSSNKPPGVK 1150
SSHQDATDPE ALWGVHQTEA DSTSEGPEDE AQRGQSTHLP RTGPGEDEDG 1200
EDTAPESALD TSLDRSFSED AVTDSSGSGT LPRVQGRVSK GTSKRRKKRP 1250
SRNQEEFVPD SDDIKAKRLC VIQ 1273
Length:1,273
Mass (Da):138,560
Last modified:October 3, 2006 - v1
Checksum:iC4438AD8A7041476
GO
Isoform 2 (identifier: Q0GNC1-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1256-1273: EFVPDSDDIKAKRLCVIQ → GLRSRPKAK

Note: No experimental confirmation available.

Show »
Length:1,264
Mass (Da):137,496
Checksum:iCC8EEF55F65801CB
GO

Sequence cautioni

The sequence AAH60610.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
The sequence AAH99931.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1256 – 127318EFVPD…LCVIQ → GLRSRPKAK in isoform 2.
VSP_021560Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1038 – 10381T → A in AAH60610. 1 Publication
Sequence conflicti1038 – 10381T → A in AAI15423. 1 Publication
Sequence conflicti1167 – 11671Q → R in AAH60610. 1 Publication
Sequence conflicti1167 – 11671Q → R in AAI15423. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ834374 mRNA. Translation: ABI20145.1.
BC060610 mRNA. Translation: AAH60610.1. Different initiation.
BC099931 mRNA. Translation: AAH99931.1. Different initiation.
BC115422 mRNA. Translation: AAI15423.2.
BC115423 mRNA. Translation: AAI15424.2.
RefSeqiNP_940803.2. NM_198411.2.
UniGeneiMm.250193.

Genome annotation databases

GeneIDi70435.
KEGGimmu:70435.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ834374 mRNA. Translation: ABI20145.1 .
BC060610 mRNA. Translation: AAH60610.1 . Different initiation.
BC099931 mRNA. Translation: AAH99931.1 . Different initiation.
BC115422 mRNA. Translation: AAI15423.2 .
BC115423 mRNA. Translation: AAI15424.2 .
RefSeqi NP_940803.2. NM_198411.2.
UniGenei Mm.250193.

3D structure databases

ProteinModelPortali Q0GNC1.
ModBasei Search...

Protein-protein interaction databases

BioGridi 214049. 1 interaction.

PTM databases

PhosphoSitei Q0GNC1.

Proteomic databases

MaxQBi Q0GNC1.
PaxDbi Q0GNC1.
PRIDEi Q0GNC1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 70435.
KEGGi mmu:70435.

Organism-specific databases

CTDi 64423.
MGIi MGI:1917685. Inf2.

Phylogenomic databases

eggNOGi NOG149898.
HOGENOMi HOG000113072.
HOVERGENi HBG081794.
InParanoidi Q0GNC1.
PhylomeDBi Q0GNC1.

Miscellaneous databases

NextBioi 331621.
PROi Q0GNC1.
SOURCEi Search...

Gene expression databases

Genevestigatori Q0GNC1.

Family and domain databases

InterProi IPR016024. ARM-type_fold.
IPR015425. FH2_Formin.
IPR010472. FH3_dom.
IPR010473. GTPase-bd.
IPR014768. GTPase-bd/formin_homology_3.
IPR027649. Inf2.
IPR003124. WH2_dom.
[Graphical view ]
PANTHERi PTHR23213:SF5. PTHR23213:SF5. 1 hit.
Pfami PF06367. Drf_FH3. 1 hit.
PF06371. Drf_GBD. 1 hit.
PF02181. FH2. 1 hit.
PF02205. WH2. 1 hit.
[Graphical view ]
SMARTi SM00498. FH2. 1 hit.
SM00246. WH2. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 1 hit.
PROSITEi PS51444. FH2. 1 hit.
PS51232. GBD_FH3. 1 hit.
PS51082. WH2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "INF2 is a WASP homology 2 motif-containing formin that severs actin filaments and accelerates both polymerization and depolymerization."
    Chhabra E.S., Higgs H.N.
    J. Biol. Chem. 281:26754-26767(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBUNIT, DOMAIN, ENZYME REGULATION, MUTAGENESIS OF LEU-1009; LEU-1010 AND LEU-1019.
    Strain: BALB/c.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 546-1273 (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 556-1273 (ISOFORM 1).
    Strain: C3H/He and C57BL/6.
    Tissue: Mesenchymal stem cell.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  4. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1172 AND SER-1174, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiINF2_MOUSE
AccessioniPrimary (citable) accession number: Q0GNC1
Secondary accession number(s): Q14C56, Q499F7, Q6P9T3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 14, 2006
Last sequence update: October 3, 2006
Last modified: May 14, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi