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Q0GNC1

- INF2_MOUSE

UniProt

Q0GNC1 - INF2_MOUSE

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Protein

Inverted formin-2

Gene

Inf2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Severs actin filaments and accelerates their polymerization and depolymerization.1 Publication

Enzyme regulationi

Phosphate inhibits both the depolymerization and severing activities.1 Publication

GO - Biological processi

  1. actin cytoskeleton organization Source: InterPro
  2. regulation of cellular component size Source: MGI
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Inverted formin-2
Gene namesi
Name:Inf2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:1917685. Inf2.

Subcellular locationi

Cytoplasmperinuclear region By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi1009 – 10091L → A: Strongly inhibits depolymerization; when associated with A-1010 and A-1019. 1 Publication
Mutagenesisi1010 – 10101L → A: Strongly inhibits depolymerization; when associated with A-1009 and A-1019. 1 Publication
Mutagenesisi1019 – 10191L → A: Strongly inhibits depolymerization; when associated with A-1009 and A-1010. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 12731272Inverted formin-2PRO_0000259890Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei1172 – 11721Phosphoserine1 Publication
Modified residuei1174 – 11741Phosphoserine1 Publication
Modified residuei1203 – 12031PhosphothreonineBy similarity
Modified residuei1216 – 12161PhosphoserineBy similarity
Modified residuei1230 – 12301PhosphothreonineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ0GNC1.
PaxDbiQ0GNC1.
PRIDEiQ0GNC1.

PTM databases

PhosphoSiteiQ0GNC1.

Expressioni

Gene expression databases

GenevestigatoriQ0GNC1.

Interactioni

Subunit structurei

Interacts with profilin and actin at the FH1 and FH2 domains respectively.1 Publication

Protein-protein interaction databases

BioGridi214049. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ0GNC1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 330329GBD/FH3PROSITE-ProRule annotationAdd
BLAST
Domaini421 – 564144FH1Add
BLAST
Domaini589 – 979391FH2PROSITE-ProRule annotationAdd
BLAST
Domaini1007 – 102216WH2PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili907 – 98478Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi421 – 563143Pro-richAdd
BLAST

Domaini

The WH2 domain acts as the DAD (diaphanous autoregulatory) domain and binds to actin monomers.1 Publication
Regulated by autoinhibition due to intramolecular GBD-DAD binding.1 Publication
The severing activity is dependent on covalent attachment of the FH2 domain to the C-terminus.1 Publication

Sequence similaritiesi

Belongs to the formin homology family.Curated
Contains 1 FH2 (formin homology 2) domain.PROSITE-ProRule annotation
Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.PROSITE-ProRule annotation
Contains 1 WH2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG149898.
HOGENOMiHOG000113072.
HOVERGENiHBG081794.
InParanoidiQ0GNC1.
PhylomeDBiQ0GNC1.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR015425. FH2_Formin.
IPR010472. FH3_dom.
IPR010473. GTPase-bd.
IPR014768. GTPase-bd/formin_homology_3.
IPR027649. Inf2.
IPR003124. WH2_dom.
[Graphical view]
PANTHERiPTHR23213:SF5. PTHR23213:SF5. 1 hit.
PfamiPF06367. Drf_FH3. 1 hit.
PF06371. Drf_GBD. 1 hit.
PF02181. FH2. 1 hit.
PF02205. WH2. 1 hit.
[Graphical view]
SMARTiSM00498. FH2. 1 hit.
SM00246. WH2. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS51444. FH2. 1 hit.
PS51232. GBD_FH3. 1 hit.
PS51082. WH2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q0GNC1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSVKEGAQRK WAALKEKLGP QDSDPTEANL ESAEPELCIR LLQMPSVVNY
60 70 80 90 100
SGLRKRLESS DGGWMVQFLE QSGLDLLLEA LARLSGRGVA RISDALLQLT
110 120 130 140 150
CISCVRAVMN SQQGIEYILS NQGYVRQLSQ ALDTSNVMVK KQVFELLAAL
160 170 180 190 200
CIYSPEGHAL TLDALDHYKM VCSQQYRFSV IMSELSDSDN VPYVVTLLSV
210 220 230 240 250
INAIILGPED LRSRAQLRSE FIGLQLLDIL TRLRDLEDAD LLIQLEAFEE
260 270 280 290 300
AKAEDEEELQ RISDGINMNS HQEVFASLFH KVSCSPASAQ LLSVLQGLMH
310 320 330 340 350
LEPAGRSGQL LWEALENLVN RAVLLASDAQ ACTLEEVVER LLSIKGRPRP
360 370 380 390 400
SPLDKAHKSV QTNSVQNQGS SSQNTTTPTT KVEGQQPVVA SPCQHVGSIQ
410 420 430 440 450
SSSVDIAPQP VALEQCITAL PLPTPPLSSS TPVLPPTPPP LPGPGATSPL
460 470 480 490 500
PPPPPPLPPP LPGSGTTSPP PPPPPPPPLP PPLPGSGTIS PPPPPPPPPL
510 520 530 540 550
PGTGAVSPPP PPPLPSLPDS HKTQPPPPPP PPLPGMCPVP PPPPLPRAGQ
560 570 580 590 600
IPPPPPLPGF SVPSMMGGVE EIIVAQVDHS LGSAWVPSHR RVNPPTLRMK
610 620 630 640 650
KLNWQKLPSN VARERNSMWA TLGSPCTAAV EPDFSSIEQL FSFPTAKPKE
660 670 680 690 700
PSAAPARKEP KEVTFLDSKK SLNLNIFLKQ FKCSNEEVTS MIQAGDTSKF
710 720 730 740 750
DVEVLKQLLK LLPEKHEIEN LRAFTEERAK LSNADQFYVL LLDIPCYPLR
760 770 780 790 800
VECMMLCEGT AIVLDMVRPK AQLVLTACES LLTSQRLPVF CQLILKIGNF
810 820 830 840 850
LNYGSHTGDA DGFKISTLLK LTETKSQQSR VTLLHHVLEE VEKSHPDLLQ
860 870 880 890 900
LSRDLEPPSQ AAGINVEIIH SEASANLKKL LEAERKVSAS IPEVQKQYAE
910 920 930 940 950
RLQASIEASQ ELDKVFDAIE QKKLELADYL CEDPQQLSLE DTFSTMKTFR
960 970 980 990 1000
DLFTRALKEN KDRKEQMAKA ERRKQQLAEE EARRPRDEDG KPIRKGPGKQ
1010 1020 1030 1040 1050
EEVCVIDALL ADIRKGFQLR KTARGRGDTE ASGRVAPTDP PKATEPATAS
1060 1070 1080 1090 1100
NPTQGTNHPA SEPLDTTAAD EPQGWDLVDA VTPSPQPSKE EDGPPALERR
1110 1120 1130 1140 1150
SSWYVDAIDF LDPEDTPDAQ PSEGVWPVTL GDGQALNPLE FSSNKPPGVK
1160 1170 1180 1190 1200
SSHQDATDPE ALWGVHQTEA DSTSEGPEDE AQRGQSTHLP RTGPGEDEDG
1210 1220 1230 1240 1250
EDTAPESALD TSLDRSFSED AVTDSSGSGT LPRVQGRVSK GTSKRRKKRP
1260 1270
SRNQEEFVPD SDDIKAKRLC VIQ
Length:1,273
Mass (Da):138,560
Last modified:October 3, 2006 - v1
Checksum:iC4438AD8A7041476
GO
Isoform 2 (identifier: Q0GNC1-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1256-1273: EFVPDSDDIKAKRLCVIQ → GLRSRPKAK

Note: No experimental confirmation available.

Show »
Length:1,264
Mass (Da):137,496
Checksum:iCC8EEF55F65801CB
GO

Sequence cautioni

The sequence AAH60610.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
The sequence AAH99931.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1038 – 10381T → A in AAH60610. (PubMed:15489334)Curated
Sequence conflicti1038 – 10381T → A in AAI15423. (PubMed:15489334)Curated
Sequence conflicti1167 – 11671Q → R in AAH60610. (PubMed:15489334)Curated
Sequence conflicti1167 – 11671Q → R in AAI15423. (PubMed:15489334)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1256 – 127318EFVPD…LCVIQ → GLRSRPKAK in isoform 2. 1 PublicationVSP_021560Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ834374 mRNA. Translation: ABI20145.1.
BC060610 mRNA. Translation: AAH60610.1. Different initiation.
BC099931 mRNA. Translation: AAH99931.1. Different initiation.
BC115422 mRNA. Translation: AAI15423.2.
BC115423 mRNA. Translation: AAI15424.2.
RefSeqiNP_940803.2. NM_198411.2.
UniGeneiMm.250193.

Genome annotation databases

GeneIDi70435.
KEGGimmu:70435.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ834374 mRNA. Translation: ABI20145.1 .
BC060610 mRNA. Translation: AAH60610.1 . Different initiation.
BC099931 mRNA. Translation: AAH99931.1 . Different initiation.
BC115422 mRNA. Translation: AAI15423.2 .
BC115423 mRNA. Translation: AAI15424.2 .
RefSeqi NP_940803.2. NM_198411.2.
UniGenei Mm.250193.

3D structure databases

ProteinModelPortali Q0GNC1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 214049. 1 interaction.

PTM databases

PhosphoSitei Q0GNC1.

Proteomic databases

MaxQBi Q0GNC1.
PaxDbi Q0GNC1.
PRIDEi Q0GNC1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 70435.
KEGGi mmu:70435.

Organism-specific databases

CTDi 64423.
MGIi MGI:1917685. Inf2.

Phylogenomic databases

eggNOGi NOG149898.
HOGENOMi HOG000113072.
HOVERGENi HBG081794.
InParanoidi Q0GNC1.
PhylomeDBi Q0GNC1.

Miscellaneous databases

NextBioi 331621.
PROi Q0GNC1.
SOURCEi Search...

Gene expression databases

Genevestigatori Q0GNC1.

Family and domain databases

InterProi IPR016024. ARM-type_fold.
IPR015425. FH2_Formin.
IPR010472. FH3_dom.
IPR010473. GTPase-bd.
IPR014768. GTPase-bd/formin_homology_3.
IPR027649. Inf2.
IPR003124. WH2_dom.
[Graphical view ]
PANTHERi PTHR23213:SF5. PTHR23213:SF5. 1 hit.
Pfami PF06367. Drf_FH3. 1 hit.
PF06371. Drf_GBD. 1 hit.
PF02181. FH2. 1 hit.
PF02205. WH2. 1 hit.
[Graphical view ]
SMARTi SM00498. FH2. 1 hit.
SM00246. WH2. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 1 hit.
PROSITEi PS51444. FH2. 1 hit.
PS51232. GBD_FH3. 1 hit.
PS51082. WH2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "INF2 is a WASP homology 2 motif-containing formin that severs actin filaments and accelerates both polymerization and depolymerization."
    Chhabra E.S., Higgs H.N.
    J. Biol. Chem. 281:26754-26767(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBUNIT, DOMAIN, ENZYME REGULATION, MUTAGENESIS OF LEU-1009; LEU-1010 AND LEU-1019.
    Strain: BALB/c.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 546-1273 (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 556-1273 (ISOFORM 1).
    Strain: C3H/He and C57BL/6.
    Tissue: Mesenchymal stem cell.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  4. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1172 AND SER-1174, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiINF2_MOUSE
AccessioniPrimary (citable) accession number: Q0GNC1
Secondary accession number(s): Q14C56, Q499F7, Q6P9T3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 14, 2006
Last sequence update: October 3, 2006
Last modified: October 29, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3