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Protein

Nucleoprotein

Gene

N

Organism
Rabies virus (strain China/MRV) (RABV)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Encapsidates the genome in a ratio of one protein N per nine ribonucleotides, protecting it from nucleases. If expressed without protein P it binds non-specifically RNA and therefore can bind it's own mRNA. Interaction with protein P abolishes any non-specific RNA binding, and prevents phosphorylation. The soluble N-P complex encapsidates specifically the genomic RNA, with protein N protecting the genome like a pearl necklace. The encapsidated genomic RNA is termed the nucleocapsid (NC) and serves as template for viral transcription and replication. Protein N binds protein P in the NC through a different interaction, and can be phosphorylated. Subsequent viral replication is dependent on intracellular concentration of newly synthesized protein N. During replication, encapsidation by protein N is coupled to RNA synthesis and all replicative products are resistant to nucleases (By similarity).By similarity

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Superantigen

Keywords - Ligandi

RNA-binding, Viral nucleoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoprotein
Short name:
NP
Alternative name(s):
Nucleocapsid protein
Short name:
Protein N
Gene namesi
Name:N
OrganismiRabies virus (strain China/MRV) (RABV)
Taxonomic identifieri445791 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesRhabdoviridaeLyssavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Mammalia [TaxID: 40674]
ProteomesiUP000008650: Genome

Subcellular locationi

Virion. Host cytoplasm By similarity

GO - Cellular componenti

  1. helical viral capsid Source: UniProtKB-KW
  2. host cell cytoplasm Source: UniProtKB-SubCell
  3. ribonucleoprotein complex Source: UniProtKB-KW
  4. viral nucleocapsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Helical capsid protein, Host cytoplasm, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 450450NucleoproteinPRO_0000295213Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei389 – 3891Phosphoserine; by host CK2By similarity

Post-translational modificationi

Phosphorylated by host CK2. Unphosphorylated protein N seems to have a better affinity for leader viral promoter encapsidation. Phosphorylation of protein N in ribonucleocapsid may stabilize the interaction with protein P, thereby playing an important role in viral transcription/replication (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homomultimerizes to form the nucleocapsid. Binds to viral genomic RNA. In nucleocapsid, binds protein P and thereby positions the polymerase on the template. Protein P acts as a chaperone on free protein N to prevent it from aggregation before encapsidating genomic RNA (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ0GBY4.
SMRiQ0GBY4. Positions 5-448.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.3570.10. 1 hit.
1.10.3610.10. 1 hit.
InterProiIPR000448. Rhabdo_ncapsid.
IPR023331. Rhabdovirus_ncapsid_C.
IPR023330. Rhabdovirus_ncapsid_N.
[Graphical view]
PfamiPF00945. Rhabdo_ncap. 1 hit.
[Graphical view]
ProDomiPD002087. Rhabd_nucleocap. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF140809. SSF140809. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0GBY4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDADKIVFKV NNQVVSLKPE IIVDQYEYKY PAIKDLKKPC ITLGKAPDLN
60 70 80 90 100
KAYKSVLSGM NAAKLDPDDV CSYLAAAMQF FEGTCPEDWT SYGILIARKG
110 120 130 140 150
DRITPNPLVE IKRTDVEGNW ALTGGMELTR DPTVSEHASL VGLLLSLYRL
160 170 180 190 200
SKISGQNTGN YKTNIADRIE QIFETAPFVK IVEHHTLMTT HKMCANWSTI
210 220 230 240 250
PNFRFLAGTY DMFFSRIEHL YSAIRVGTVV TAYEDCSGLV SFTGFIKQIN
260 270 280 290 300
LTAREAILYF FHKNFEEEIR RMFEPGQETA VPHSYFIHFR SLGLSGKSPY
310 320 330 340 350
SSNAVGHVFN LIHFVGCYMG QVRSLNATVI AACAPHEMSV LGGYLGEEFF
360 370 380 390 400
GKGTFERRFF RDEKELQEYE AAELTKSDVA LADDGTVNSD DEDYFSGETR
410 420 430 440 450
SPEAVYTRIM MNGGRLKRSH IRRYVSVSSN HQARPNSFAE FLNKTYSNDS
Length:450
Mass (Da):50,744
Last modified:October 3, 2006 - v1
Checksum:i6D7911DAA222A86A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ875050 Genomic RNA. Translation: ABI47937.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ875050 Genomic RNA. Translation: ABI47937.1.

3D structure databases

ProteinModelPortaliQ0GBY4.
SMRiQ0GBY4. Positions 5-448.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di1.10.3570.10. 1 hit.
1.10.3610.10. 1 hit.
InterProiIPR000448. Rhabdo_ncapsid.
IPR023331. Rhabdovirus_ncapsid_C.
IPR023330. Rhabdovirus_ncapsid_N.
[Graphical view]
PfamiPF00945. Rhabdo_ncap. 1 hit.
[Graphical view]
ProDomiPD002087. Rhabd_nucleocap. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF140809. SSF140809. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Zhao Y.J., Guo L., Huang Y., Qian A.D.
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiNCAP_RABVR
AccessioniPrimary (citable) accession number: Q0GBY4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: October 3, 2006
Last modified: February 4, 2015
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Displays a superantigen activity in human and mouse, activating mostly V-beta-8 subtypes of T-cell receptor.By similarity

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.