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Protein

Serine/threonine-protein phosphatase 2B catalytic subunit

Gene

tax-6

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-dependent, calmodulin-stimulated protein phosphatase (PubMed:12221132, PubMed:16481400, PubMed:22300764, PubMed:23805378). Dephosphorylates arrd-17 (PubMed:22300764). Dephosphorylates daf-16 at 'Ser-319' which regulates daf-16 nuclear translocation (PubMed:23805378). Dephosphorylates calcium permeable cation channel pkd-2 at 'Ser-534' (PubMed:16481400). Regulates male mating behavior including response to hermaphrodite contact and vulva location and localization of pkd-2 to neuronal cilium (PubMed:16481400). Negatively regulates several sensory behaviors including thermotaxis in ADF neurons, osmosensation in ASH neurons, olfaction adaptation in AWC neurons and sensitivity to CO2 levels (PubMed:18524955, PubMed:11879652). Plays a role in egg-laying, body-size, fertility, growth, movement and cuticle development (PubMed:12684004, PubMed:15522306, PubMed:17113567, PubMed:22300764). Negatively regulates lifespan (PubMed:19279398). Promotes transcription activator crtc-1 nuclear localization, probably by dephosphorylating crtc-1 and thereby negatively regulates lifespan (PubMed:21331044). Regulates expression of chemoreceptor srt-2 in AWC neurons probably downstream of Ser/Thr kinase kin-29 (PubMed:17113567). Regulates expression of tax-6 inhibitor rcn-1 (PubMed:12684004). Negatively regulates nicotinic acetylcholine receptor (nAChR) sensitivity to nicotine (PubMed:15990870). Plays a role in several endocytic processes including in coelomocyte endocytosis, intestine apical endocytosis and synaptic vesicle recycling (PubMed:20803083, PubMed:21345307). May negatively regulate excitatory GABA receptor exp-1 during enteric muscle contraction (PubMed:16084527). May negatively regulate autophagy (PubMed:19279398).15 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.UniRule annotation4 Publications

Cofactori

Protein has several cofactor binding sites:
  • Fe3+CuratedNote: Binds 1 Fe3+ ion per subunit.Curated
  • Zn2+CuratedNote: Binds 1 zinc ion per subunit.Curated

Enzyme regulationi

Inhibited by immunosuppressant cyclosporin A (CsA).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi115IronBy similarity1
Metal bindingi117IronBy similarity1
Metal bindingi143IronBy similarity1
Metal bindingi143ZincBy similarity1
Metal bindingi175ZincBy similarity1
Active sitei176Proton donorBy similarity1
Metal bindingi224ZincBy similarity1
Metal bindingi306ZincBy similarity1

GO - Molecular functioni

  • calcium ion binding Source: WormBase
  • calmodulin-dependent protein phosphatase activity Source: WormBase
  • phosphoprotein phosphatase activity Source: WormBase

GO - Biological processi

  • calcium-mediated signaling Source: UniProtKB
  • chemosensory behavior Source: UniProtKB
  • chemotaxis Source: UniProtKB
  • dauer larval development Source: WormBase
  • determination of adult lifespan Source: WormBase
  • endocytosis Source: UniProtKB-KW
  • hyperosmotic response Source: WormBase
  • olfactory behavior Source: WormBase
  • olfactory learning Source: WormBase
  • peptidyl-serine dephosphorylation Source: WormBase
  • positive regulation of growth rate Source: WormBase
  • positive regulation of multicellular organism growth Source: WormBase
  • protein dephosphorylation Source: WormBase
  • protein localization to non-motile cilium Source: WormBase
  • regulation of gene expression Source: WormBase
  • response to alkaline pH Source: UniProtKB
  • response to hermaphrodite contact Source: WormBase
  • thermosensory behavior Source: WormBase
  • thermotaxis Source: WormBase
  • vulval location Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Behavior, Chemotaxis, Endocytosis

Keywords - Ligandi

Calcium, Iron, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-CEL-2871809. FCERI mediated Ca+2 mobilization.
R-CEL-4086398. Ca2+ pathway.
R-CEL-5607763. CLEC7A (Dectin-1) induces NFAT activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 2B catalytic subunitCurated (EC:3.1.3.16UniRule annotation4 Publications)
Alternative name(s):
Abnormal chemotaxis protein 6Imported
Calmodulin-dependent calcineurin subunit A1 Publication
Gene namesi
Name:tax-6Imported
Synonyms:cna-1Imported
ORF Names:C02F4.2Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiC02F4.2a; CE07853; WBGene00006527; tax-6.
C02F4.2b; CE46805; WBGene00006527; tax-6.
C02F4.2c; CE46586; WBGene00006527; tax-6.

Subcellular locationi

Isoform a :
  • Perikaryon 1 Publication
  • Cell projectiondendrite 1 Publication
  • Cell projectionaxon 1 Publication
  • Cell projectioncilium 1 Publication
  • Cytoplasm 1 Publication

GO - Cellular componenti

  • axon Source: WormBase
  • cytoplasm Source: WormBase
  • dendrite Source: WormBase
  • neuronal cell body Source: WormBase
  • non-motile cilium Source: WormBase
  • nucleus Source: WormBase
  • perikaryon Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown results in an increase in lifespan (PubMed:21331044, PubMed:23805378). Also causes nuclear exclusion of crtc-1 (PubMed:21331044).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi259D → N in p675; impaired thermotaxis and chemotaxis towards NaCl, hypersensitivity to low osmotic conditions, enhanced olfactory adaptation to isoamyl alcohol. Slow growth rate and body is smaller and slender with a transparent appearance resulting from a thinner cuticle. Reduced brood size associated with egg retention. Partially resistant to serotonin-induced egg laying. Increased susceptibility to nicotine-mediated paralysis. Impaired mating behavior and reduced localization of pkd-2 to neuronal cilium. Impaired CO2 avoidance. Impaired coelomocyte endocytosis, apical endocytosis in intestine and recycling of synaptic vesicles at synapses. 7 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004369041 – 542Serine/threonine-protein phosphatase 2B catalytic subunitCuratedAdd BLAST542

Proteomic databases

EPDiQ0G819.
PaxDbiQ0G819.
Q86MD4.
Q95002.
PeptideAtlasiQ0G819.

Expressioni

Tissue specificityi

Expressed in head and tail neurons, ventral nerve cord, body wall and vulva muscles, sperm and spermatheca (PubMed:12221132). Expressed in hypodermal seam cells (PubMed:15522306). Expressed in intestinal and anal depressor muscles (PubMed:16084527). In male, expressed in CEM, HOB and ray RnB neurons (PubMed:16481400). Isoform a: Expressed in thermosensory ADF neurons, chemosensory ASE, AWA and AWC neurons, osmosensory ASH neurons and AIY and AIZ interneurons (PubMed:11879652). Expressed in body wall, vulva and pharyngeal muscles (PubMed:11879652).5 Publications

Developmental stagei

Expressed in embryos and at all larval stages.1 Publication

Gene expression databases

BgeeiWBGene00006527.
ExpressionAtlasiQ0G819. differential.

Interactioni

Subunit structurei

Forms a complex composed of a catalytic subunit tax-6 and a regulatory subunit cnb-1 which confers calcium sensitivity (PubMed:12221132). Interacts with rcn-1; the interaction is calcium-dependent and inhibits tax-6 catalytic activity (PubMed:12684004). Interacts with exp-1; the interaction is calcium and cnb-1-independent (PubMed:16084527). Interacts with arrd-17 (PubMed:22300764). Interacts with kin-29 (PubMed:17113567). Interacts with cnp-2 (PubMed:18593529). Interacts with daf-16 (PubMed:23805378). Interacts with dyn-1 (PubMed:20803083).8 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
kin-29Q210173EBI-323063,EBI-2893174
rcn-1P538065EBI-323063,EBI-323055

Protein-protein interaction databases

DIPiDIP-25135N.
IntActiQ0G819. 4 interactors.
MINTiMINT-1093406.
STRINGi6239.C02F4.2c.

Structurei

3D structure databases

ProteinModelPortaliQ0G819.
SMRiQ0G819.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni38 – 326CatalyticCuratedAdd BLAST289
Regioni272 – 278Calcineurin B binding-site 1Curated7
Regioni321 – 326Calcineurin B binding-site 2Curated6
Regioni418 – 440Calmodulin-bindingCuratedAdd BLAST23
Regioni490 – 512Inhibitory domainCuratedAdd BLAST23

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-2B subfamily.Curated

Phylogenomic databases

eggNOGiKOG0375. Eukaryota.
COG0639. LUCA.
GeneTreeiENSGT00530000063087.
HOGENOMiHOG000172699.
InParanoidiQ0G819.
KOiK04348.
OMAiRRIDRFK.
OrthoDBiEOG091G094R.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform cImported (identifier: Q0G819-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASTSAGQNS AAKPDTTNTT TTASNNNRER ERDLKQFTER FVKTVQFPVS
60 70 80 90 100
ERLTVDQVYD RRTGKPRHEV LRDHFIKEGR IEEEAAIRVI QECSSLFRNE
110 120 130 140 150
KTMLEIEAPV TVCGDIHGQF YDLMKLFEVG GSPATTKYLF LGDYVDRGYF
160 170 180 190 200
SIECVLYLWA LKICYPTTLF LLRGNHECRH LTEYFTFKQE CKIKYSERVY
210 220 230 240 250
DVCMESFDAL PLAALMNQQF LCVHGGLSPE IHTLEDIRRI DRFKEPPAFG
260 270 280 290 300
PMCDLLWSDP LEDFGNERNS EQFSHNSVRG CSYFYSYAAC CDFLQHNNLL
310 320 330 340 350
SIIRAHEAQD AGYRMYRKSQ ATGFPSLITI FSAPNYLDVY NNKAAILKYE
360 370 380 390 400
NNVMNIRQFN CSPHPYWLPN FMDVFTWSLP FVGEKVTEML VHILNICSDD
410 420 430 440 450
ELMAECDDTF EGGVGSARKE VIRHKIRAIG KMARAFSVLR HESESVLQLK
460 470 480 490 500
GLNAGGKLPQ GALSEGRTGL NAAYRIEQSV AAESGCDSGH LIQSFEEARR
510 520 530 540
LDKINERMPP TMATPPAQSP SQSPIPSRQT TPQPPQNGPS NS
Note: No experimental confirmation available.Curated
Length:542
Mass (Da):61,505
Last modified:January 25, 2012 - v2
Checksum:i60983B8A0C504883
GO
Isoform aImported (identifier: Q0G819-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     441-441: H → E
     448-479: QLKGLNAGGKLPQGALSEGRTGLNAAYRIEQS → ALKGLTPTGALPMGTLQGGSRGVRE

Show »
Length:535
Mass (Da):60,666
Checksum:iFC0363809D04103E
GO
Isoform bImported (identifier: Q0G819-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     413-450: GVGSARKEVI...HESESVLQLK → ADGKWIFPYP...IFYQTFFGTP
     451-542: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:450
Mass (Da):52,057
Checksum:iEC01B58E6C8F3E43
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_058441413 – 450GVGSA…VLQLK → ADGKWIFPYPPHTGDVCLTI RFSFIFSLIFYQTFFGTP in isoform b. Add BLAST38
Alternative sequenceiVSP_058442441H → E in isoform a. Curated1
Alternative sequenceiVSP_058443448 – 479QLKGL…RIEQS → ALKGLTPTGALPMGTLQGGS RGVRE in isoform a. Add BLAST32
Alternative sequenceiVSP_058444451 – 542Missing in isoform b. CuratedAdd BLAST92

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284604 Genomic DNA. Translation: CAB02719.1.
BX284604 Genomic DNA. Translation: CAD88213.2.
BX284604 Genomic DNA. Translation: CAL36491.2.
PIRiT18864.
RefSeqiNP_001021292.1. NM_001026121.1. [Q0G819-2]
NP_001021293.2. NM_001026122.3. [Q0G819-3]
NP_001076658.2. NM_001083189.4. [Q0G819-1]
UniGeneiCel.13613.

Genome annotation databases

EnsemblMetazoaiC02F4.2a; C02F4.2a; WBGene00006527. [Q0G819-2]
C02F4.2b; C02F4.2b; WBGene00006527. [Q0G819-3]
C02F4.2c; C02F4.2c; WBGene00006527. [Q0G819-1]
GeneIDi177943.
KEGGicel:CELE_C02F4.2.
UCSCiC02F4.2c. c. elegans. [Q0G819-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284604 Genomic DNA. Translation: CAB02719.1.
BX284604 Genomic DNA. Translation: CAD88213.2.
BX284604 Genomic DNA. Translation: CAL36491.2.
PIRiT18864.
RefSeqiNP_001021292.1. NM_001026121.1. [Q0G819-2]
NP_001021293.2. NM_001026122.3. [Q0G819-3]
NP_001076658.2. NM_001083189.4. [Q0G819-1]
UniGeneiCel.13613.

3D structure databases

ProteinModelPortaliQ0G819.
SMRiQ0G819.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-25135N.
IntActiQ0G819. 4 interactors.
MINTiMINT-1093406.
STRINGi6239.C02F4.2c.

Proteomic databases

EPDiQ0G819.
PaxDbiQ0G819.
Q86MD4.
Q95002.
PeptideAtlasiQ0G819.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC02F4.2a; C02F4.2a; WBGene00006527. [Q0G819-2]
C02F4.2b; C02F4.2b; WBGene00006527. [Q0G819-3]
C02F4.2c; C02F4.2c; WBGene00006527. [Q0G819-1]
GeneIDi177943.
KEGGicel:CELE_C02F4.2.
UCSCiC02F4.2c. c. elegans. [Q0G819-1]

Organism-specific databases

CTDi177943.
WormBaseiC02F4.2a; CE07853; WBGene00006527; tax-6.
C02F4.2b; CE46805; WBGene00006527; tax-6.
C02F4.2c; CE46586; WBGene00006527; tax-6.

Phylogenomic databases

eggNOGiKOG0375. Eukaryota.
COG0639. LUCA.
GeneTreeiENSGT00530000063087.
HOGENOMiHOG000172699.
InParanoidiQ0G819.
KOiK04348.
OMAiRRIDRFK.
OrthoDBiEOG091G094R.

Enzyme and pathway databases

ReactomeiR-CEL-2871809. FCERI mediated Ca+2 mobilization.
R-CEL-4086398. Ca2+ pathway.
R-CEL-5607763. CLEC7A (Dectin-1) induces NFAT activation.

Miscellaneous databases

PROiQ0G819.

Gene expression databases

BgeeiWBGene00006527.
ExpressionAtlasiQ0G819. differential.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPP2B_CAEEL
AccessioniPrimary (citable) accession number: Q0G819
Secondary accession number(s): Q86MD4, Q95002
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 6, 2016
Last sequence update: January 25, 2012
Last modified: November 30, 2016
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.