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Protein
Submitted name:

Polyprotein

Gene
N/A
Organism
Turnip mosaic virus (strain Japanese) (TuMV)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Helper component proteinase: required for aphid transmission and also has proteolytic activity. Only cleaves a Gly-Gly dipeptide at its own C-terminus. Interacts with virions and aphid stylets. Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. May have RNA-binding activity.SAAS annotation

Catalytic activityi

Hydrolyzes a Gly-|-Gly bond at its own C-terminus, commonly in the sequence -Tyr-Xaa-Val-Gly-|-Gly, in the processing of the potyviral polyprotein.SAAS annotation
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).SAAS annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei706For helper component proteinase activityPROSITE-ProRule annotation1
Active sitei779For helper component proteinase activityPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Nucleotidyltransferase, ProteaseSAAS annotation, RNA-directed RNA polymeraseSAAS annotation, Suppressor of RNA silencingSAAS annotation, Transferase
Biological processViral RNA replicationSAAS annotation
LigandATP-bindingSAAS annotation, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Submitted name:
PolyproteinImported
OrganismiTurnip mosaic virus (strain Japanese) (TuMV)Imported
Taxonomic identifieri12230 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stagePotyviridaePotyvirus
Virus hostiAlliaria petiolata (Garlic mustard) (Arabis petiolata) [TaxID: 126270]
Brassica [TaxID: 3705]
Calanthe [TaxID: 38206]
Capsella bursa-pastoris (Shepherd's purse) (Thlaspi bursa-pastoris) [TaxID: 3719]
Hesperis matronalis [TaxID: 264418]
Stellaria media (Common chickweed) (Alsine media) [TaxID: 13274]
Trifolium hybridum (Alsike clover) [TaxID: 74517]

Subcellular locationi

GO - Cellular componenti

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini698 – 820Peptidase C6InterPro annotationAdd BLAST123
Domaini1300 – 1452Helicase ATP-bindingInterPro annotationAdd BLAST153
Domaini1471 – 1630Helicase C-terminalInterPro annotationAdd BLAST160
Domaini2117 – 2335Peptidase C4InterPro annotationAdd BLAST219
Domaini2601 – 2725RdRp catalyticInterPro annotationAdd BLAST125

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili2883 – 2914Sequence analysisAdd BLAST32

Sequence similaritiesi

Belongs to the potyviridae genome polyprotein family.UniRule annotation

Keywords - Domaini

Coiled coilSequence analysis

Family and domain databases

InterProiView protein in InterPro
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR001456. HC-pro.
IPR031159. HC_PRO_CPD_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR002540. Pept_S30_P1_potyvir.
IPR009003. Peptidase_S1_PA.
IPR001592. Poty_coat.
IPR001730. Potyv_NIa-pro_dom.
IPR013648. PP_Potyviridae.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
PfamiView protein in Pfam
PF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00863. Peptidase_C4. 1 hit.
PF00851. Peptidase_C6. 1 hit.
PF01577. Peptidase_S30. 1 hit.
PF00767. Poty_coat. 1 hit.
PF08440. Poty_PP. 1 hit.
PF00680. RdRP_1. 1 hit.
PRINTSiPR00966. NIAPOTYPTASE.
SMARTiView protein in SMART
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiView protein in PROSITE
PS51744. HC_PRO_CPD. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51436. POTYVIRUS_NIA_PRO. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0EDF4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAVTFATAI TNTTASRSAS TGMVQFGNFP SVPLRSTVAT TATTSVVQPK
60 70 80 90 100
LYTVQFGSLE PIVVKGKAGS SAKATCQQPS GEINASLKEA AALEVAKPKP
110 120 130 140 150
NAVLRMHEEM NKERALFLDW ETSLTRRSYG IADNEKIIMT ARGVSKIVLR
160 170 180 190 200
SSRAMKQKRA RERRRAQQPI VLKWEPKLSE ISIGRGPSAN MIEAEEVRTK
210 220 230 240 250
WPLHKTPSIK KKTVHKRCKM NDQGIDMLMR SLIKIFKAKS ANIEFIGRKS
260 270 280 290 300
IKVDFVKKEQ TKFARVQVVH LLGKRAQRDL LTGAEENHFI DTLSTYSGNR
310 320 330 340 350
RIINPGVVCA GWSGIVIRNG ILTQKQSRSP SQAFVVRGEH EGKLYDARVK
360 370 380 390 400
VTKVMSHKII HYSAAGANFW KGFDRCFLAY RGDNREHTCY TGLDVTECGE
410 420 430 440 450
VAALMCLAMF PCGKITCPDC VTDSELSQGQ ASGPSIKHKL AQLREVIKSS
460 470 480 490 500
YPRFKHAVQI LDRYEQSLSS ANENYQDFAE IQSISDGTDK AAFPHINKLN
510 520 530 540 550
AILIKGATAT GEEFSQATKY LLEIARYLKN RTENIEKGSL KSFRNKISQK
560 570 580 590 600
AHINPTLMCD NQLDKNGNFI WGERGYHAKR FFSNYFEIID PKKGYTQYET
610 620 630 640 650
RIVPNGSRKL AIGKLIVPTN FEVLREQMKG EPIEPHPITV ECVSKLQGDF
660 670 680 690 700
VHACCCVTTE SGDPVLSEIK MPTKHHLVIG NSGDPKYIDL PEIEENKMYI
710 720 730 740 750
AKEGYCYINI FLAMLVNVKE SQAKEFTKVV RDKLVGELGK WPTLLDVATA
760 770 780 790 800
CYFLKVFYPD VANAELPRML VDHKTKIIHV VDSYGSLSTG YHILKTNTVE
810 820 830 840 850
QLIKFTRCNL ESSLKHYRVG GTAWEEAHGF NNIDNPQWCI KRLIQGVYRP
860 870 880 890 900
KKLREDMLTN PFLPLYALLS PGVILAFYNS GSLEYLMNHY IRADSNVAVL
910 920 930 940 950
LVVLKSLAKK VSTSQSVLAQ LQIIERSLPE LVEARANITG PDEAASQACN
960 970 980 990 1000
RFLGMLIHMA EPNNELADGG YTILRDHSIS ILEKSYLQIL DEAWSELSWS
1010 1020 1030 1040 1050
ERCAIKYYSS KQAIFSQKDL QMRSDVDLGG RYSESVMSSY EWGKQRVKSV
1060 1070 1080 1090 1100
YSNACNKVRS SVSWTSSKVS SSVCKTINYL VPDVFKFINV LVCISLLVTI
1110 1120 1130 1140 1150
AAEANRIVTT QRRLKLDIEE TERKKIEWEL AFHHAILTQS AGQHPTLDEF
1160 1170 1180 1190 1200
TAYIGEKAPH LSEHIEPEEK AVVHQAKRQS EQELERVIAF IALVLMMFDA
1210 1220 1230 1240 1250
ERSDCVTKIL NKLKGLVSTV EPAVYHQTLN DIEDDLSERN LFVDFELSND
1260 1270 1280 1290 1300
GEILQQLPAE KTFASWWNHQ LSRGFTIPHY RTEGKFMTFT RATATEVAGK
1310 1320 1330 1340 1350
IAHESDRDIL LMGAVGSGKS TGLPYHLSRK GNVLLLEPTR PLAENVHKQL
1360 1370 1380 1390 1400
SQAPFHQNTT LRMRGLTSFG SAPISVMTSG FALNYFANNR TRIEEFDFVI
1410 1420 1430 1440 1450
FDECHVHDAN AMAMRCLLHE CDYSGKVIKV SATPPGREVE FSTQYPVTIS
1460 1470 1480 1490 1500
TEDTLSFQDF VNAQGSGSNC DVISKGDNIL VYVASYNEVD TLSKLLVERD
1510 1520 1530 1540 1550
FKVTKVDGRT MKVGNIEITT SGTPSRKHFI VATNIIENGV TLDIDVVADF
1560 1570 1580 1590 1600
GTKVLPYLDT DNRMLSTTKT SINYGERIQR LGRVGRHKPG HALRIGHTEK
1610 1620 1630 1640 1650
GLSEVPSCIA TEAALKCFTY GLPVITNNVS TSILGNVTVK QARTMSVFEI
1660 1670 1680 1690 1700
TPFYTSQVVR YDGSMHPQVH ALLKRFKLRD SEIVLNKLAI PNRGVNAWLT
1710 1720 1730 1740 1750
ASEYARLGAN VEDRRDVRIP FMCRDIPEKL HLEMWDVIVK FKGDAGFGRL
1760 1770 1780 1790 1800
SSASASKVAY TLQTDVNSIQ RTVTIIDTLI AEERRKQEYF KTVTSNCVSS
1810 1820 1830 1840 1850
SNFSLQSITN AIKSRMMKDH TCENISVLEG AKSQLLEFRN LNADHSFTTK
1860 1870 1880 1890 1900
SDGISRHFMS EYGALEAVHH QNTNDMSKFL KLKGKWNKTL ITRDVLVICG
1910 1920 1930 1940 1950
VLGGGIWMII QHLRMKISEP VTHEAKGKRQ RQKLKFRNAR DNKMGREVYG
1960 1970 1980 1990 2000
EDDVIEHFFG DAYTKKGKSK GRTRGLGHKN RKFINMYGFD PEDFSAVRFV
2010 2020 2030 2040 2050
DPLTGATIDE SPIMDIALVQ EHFGKIRMDL LGEDELEPDE LRMNKTIQAY
2060 2070 2080 2090 2100
YMNNKTGKAL KVDLTPHVPL KVCDLHATIA GFPEREHELR QTGKAQPIDL
2110 2120 2130 2140 2150
SEVPRANTEL TPVDHESSSM FRGLRDYNPI SNNICHLTNI SDGASNSLYG
2160 2170 2180 2190 2200
VGFGPLILTN RHLFERNNGE LVIKSRHGEF VIKNTTQLHL LPIPDRDLLL
2210 2220 2230 2240 2250
IRLPKDIPPF PQKLGFRQPE KGERICMVGS NFQTKSITSV VSETSTIMPV
2260 2270 2280 2290 2300
ENSQFWKHWI STKDGQCGRP MVSTKDGKIL GLHSLANFQN SINYFAAFPD
2310 2320 2330 2340 2350
DFAEKYLYTI EAHEWVKHWK YNTSAISWGS LNIQASQPAG LFKVSKLISD
2360 2370 2380 2390 2400
LDSTAVYAQT QQNRWMYEQL NGNLKAIAHC PSQLVTKHTV KGKCQMFDLY
2410 2420 2430 2440 2450
LKLHDEAREY FQPMLGQYQK SRLNREAYAK DLLKYATPIE AGNIDCDLFE
2460 2470 2480 2490 2500
KTVETVISDL RGYGFETCNY VTDEIDIFEA LNMKSAVGAL YKGKKKDYFA
2510 2520 2530 2540 2550
EFTPEMKEEI LKQSCERLFL GKMGVWNGSL KAELRPLEKV EANKTRTFTA
2560 2570 2580 2590 2600
APLDTLLGGK VCVDDFNNQF YDHNLRAPWS VGMTKFYCGW DRLLESLPDG
2610 2620 2630 2640 2650
WIYCDADGSQ FDSSLSPYLI NAVLNIRLEF MEEWDIGEVM LRNLYTEIVY
2660 2670 2680 2690 2700
TPISTPDGTL VKKFKGNNSG QPSTVVDNTL MVILAVNYSL RKSGIPNELR
2710 2720 2730 2740 2750
DNLIRFFVNG DDLLLGVHPK YEYVLDTMAD NFRELGLKYT FDSRTRRKGD
2760 2770 2780 2790 2800
LWFMSHQGHK REGIWIPKLE PERIVSILEW DRSKEPCHRL EAICAAMIES
2810 2820 2830 2840 2850
WGYDKLTHEI RKFYAWMIEQ APYSSLAQEG KAPYIAETAL RKLYLDKEPA
2860 2870 2880 2890 2900
QEDLTQYLQA IFEDYEDGAE VCVYHQAGET LDAVLTEEQK QAEKERKERE
2910 2920 2930 2940 2950
RSEKERERQR QLALKKGKNA AQEEGERDNE VNAGTSGTFS VPRLKSLTSK
2960 2970 2980 2990 3000
MRVPKYEKRV ALNLDHLILY TPEQTDLSNT RSTRKQFDTW FEGVMADYEL
3010 3020 3030 3040 3050
TEDKMHIILN GLMVWCIENG TSPNINGMWV MMDGDDQVEF PIKPLIDHAK
3060 3070 3080 3090 3100
PTFRQIMAHF SDVAEAYIEK RNQDRPYMPR YGLQRNLTDM SLARYAFDFY
3110 3120 3130 3140 3150
EMTSRTPIRA REAHIQMKAA ALRGANNNLF GLDGNVGTTV ENTERHTTED
3160
VNRNMHNLLG VKGL
Length:3,164
Mass (Da):358,117
Last modified:October 17, 2006 - v1
Checksum:iD1AF7EE2AE82098D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB252103 Genomic RNA. Translation: BAF31148.1.

Similar proteinsi

Entry informationi

Entry nameiQ0EDF4_TUMVJ
AccessioniPrimary (citable) accession number: Q0EDF4
Entry historyiIntegrated into UniProtKB/TrEMBL: October 17, 2006
Last sequence update: October 17, 2006
Last modified: May 10, 2017
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)