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Q0ED31

- Q0ED31_9HIV1

UniProt

Q0ED31 - Q0ED31_9HIV1

Protein

Envelope glycoprotein gp160

Gene

env

Organism
Human immunodeficiency virus 1
Status
Unreviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (17 Oct 2006)
      Previous versions | rss
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    Functioni

    The envelope glyprotein gp160 precursor down-modulates cell surface CD4 antigen by interacting with it in the endoplasmic reticulum and blocking its transport to the cell surface.UniRule annotationSAAS annotation
    The gp120-gp41 heterodimer allows rapid transcytosis of the virus through CD4 negative cells such as simple epithelial monolayers of the intestinal, rectal and endocervical epithelial barriers. Both gp120 and gp41 specifically recognize glycosphingolipids galactosyl-ceramide (GalCer) or 3' sulfo-galactosyl-ceramide (GalS) present in the lipid rafts structures of epithelial cells. Binding to these alternative receptors allows the rapid transcytosis of the virus through the epithelial cells. This transcytotic vesicle-mediated transport of virions from the apical side to the basolateral side of the epithelial cells does not involve infection of the cells themselves.SAAS annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei61 – 611MannoseImported

    GO - Molecular functioni

    1. structural molecule activity Source: InterPro

    GO - Biological processi

    1. apoptotic process Source: UniProtKB-KW
    2. evasion or tolerance by virus of host immune response Source: UniProtKB-KW
    3. membrane fusion involved in viral entry into host cell Source: UniProtKB-KW
    4. virion attachment to host cell Source: UniProtKB-KW

    Keywords - Biological processi

    ApoptosisSAAS annotation, Fusion of virus membrane with host membraneSAAS annotation, Host-virus interaction, Viral attachment to host cellUniRule annotationSAAS annotation, Viral immunoevasionUniRule annotation, Viral penetration into host cytoplasm, Virus entry into host cell

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Envelope glycoprotein gp160UniRule annotation
    Gene namesi
    Name:envImported
    OrganismiHuman immunodeficiency virus 1Imported
    Taxonomic identifieri11676 [NCBI]
    Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeLentivirusPrimate lentivirus group
    Virus hostiHomo sapiens (Human) [TaxID: 9606]

    Subcellular locationi

    Virion membrane SAAS annotation; Single-pass type I membrane protein SAAS annotation. Host cell membrane SAAS annotation; Single-pass type I membrane protein SAAS annotation. Host endosome membrane SAAS annotation; Single-pass type I membrane protein SAAS annotation

    GO - Cellular componenti

    1. host cell endosome membrane Source: UniProtKB-SubCell
    2. host cell plasma membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW
    4. viral envelope Source: UniProtKB-KW
    5. virion membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cell membraneSAAS annotation, Host endosomeSAAS annotation, Host membrane, Membrane, Viral envelope proteinUniRule annotationSAAS annotation, Virion

    Pathology & Biotechi

    Keywords - Diseasei

    AIDSUniRule annotation

    PTM / Processingi

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi87 – 871N-linked (GlcNAc...)Imported
    Glycosylationi160 – 1601N-linked (GlcNAc...)Imported
    Glycosylationi188 – 1881N-linked (GlcNAc...)Imported
    Glycosylationi236 – 2361N-linked (GlcNAc...)Imported
    Glycosylationi243 – 2431N-linked (GlcNAc...)Imported
    Glycosylationi264 – 2641N-linked (GlcNAc...)Imported
    Glycosylationi278 – 2781N-linked (GlcNAc...)Imported
    Glycosylationi291 – 2911N-linked (GlcNAc...)Imported
    Glycosylationi297 – 2971N-linked (GlcNAc...)Imported
    Glycosylationi335 – 3351N-linked (GlcNAc...)Imported
    Glycosylationi356 – 3561N-linked (GlcNAc...)Imported
    Glycosylationi386 – 3861N-linked (GlcNAc...)Imported
    Glycosylationi392 – 3921N-linked (GlcNAc...)Imported
    Glycosylationi442 – 4421N-linked (GlcNAc...)Imported

    Keywords - PTMi

    Cleavage on pair of basic residuesUniRule annotation, Disulfide bondSAAS annotation

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3NGBX-ray2.68A/D/G/I201-303[»]
    A/D/G/I325-486[»]
    A/D/G/I43-122[»]
    3SE8X-ray1.90G43-486[»]
    3SE9X-ray2.00G43-486[»]
    3TGTX-ray1.90A43-486[»]
    3U7YX-ray2.45G201-303[»]
    G325-486[»]
    G43-122[»]
    4DKOX-ray1.98A/C43-486[»]
    4DKPX-ray1.80A/C43-486[»]
    4DKQX-ray1.89A43-486[»]
    4DKRX-ray1.80A/C43-486[»]
    4DKUX-ray2.49A/B201-303[»]
    A/B325-486[»]
    A/B43-122[»]
    4DKVX-ray2.18A/B201-303[»]
    A/B325-486[»]
    A/B43-122[»]
    4DVSX-ray2.10A/B201-302[»]
    A/B325-486[»]
    A/B43-122[»]
    4DVTX-ray2.40A/B201-293[»]
    A/B457-486[»]
    A/B88-122[»]
    4DVVX-ray1.94A/B201-303[»]
    A/B325-486[»]
    A/B43-122[»]
    4DVWX-ray2.20A/B201-433[»]
    A/B325-486[»]
    A/B43-122[»]
    4DVXX-ray2.40A/B201-303[»]
    A/B325-486[»]
    A/B43-122[»]
    4H8WX-ray1.85G43-486[»]
    4I54X-ray2.50A/B43-486[»]
    4JB9X-ray2.60G43-486[»]
    4JDTX-ray3.26G43-486[»]
    4JKPX-ray2.82G43-486[»]
    4JPVX-ray2.83G43-486[»]
    4JPWX-ray2.90G43-486[»]
    4LSPX-ray2.15G43-486[»]
    4LSQX-ray2.25G43-486[»]
    4LSRX-ray2.28G43-486[»]
    4LSUX-ray2.30G43-486[»]
    4P9HX-ray3.00G201-303[»]
    G325-486[»]
    G43-122[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ0ED31.

    Family & Domainsi

    Keywords - Domaini

    Transmembrane, Transmembrane helixSAAS annotation

    Family and domain databases

    Gene3Di2.170.40.20. 2 hits.
    InterProiIPR000777. HIV1_GP160.
    IPR000328. Retroviral_envelope_protein.
    [Graphical view]
    PfamiPF00516. GP120. 1 hit.
    PF00517. GP41. 1 hit.
    [Graphical view]
    SUPFAMiSSF56502. SSF56502. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Q0ED31-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRVKETQMNW PNLWKWGTLI LGLVIICSAS DNLWVTVYYG VPVWKDADTT    50
    LFCASDAKAH ETEVHNVWAT HACVPTDPNP QEIHLENVTE NFNMWKNNMV 100
    EQMQEDVISL WDQSLQPCVK LTPLCVTLHC TTAKLTNVTN ITNVPNIGNI 150
    TDEVRNCSFN MTTEIRDKKQ KVHALFYKLD IVQIEDKNDS SKYRLINCNT 200
    SVIKQACPKI SFDPIPIHYC TPAGYVILKC NDKNFNGTGP CKNVSSVQCT 250
    HGIKPVVSTQ LLLNGSLAEE EIIIRSENLT NNAKTIIVHL NKSVEINCTR 300
    PSNNMRTSMR IGPGQVFYRT GSITGDIRKA YCEINGTKWN KVLKQVTEKL 350
    KEHFNNKTII FQPPSGGDLE ITMHHFNCRG EFFYCNTTQL FNNTCIGNET 400
    MKGCNGTITL PCKIKQIINM WQGTGQAMYA PPIDGKINCV SNITGILLTR 450
    DGGANNTSNE TFRPGGGNIK DNWRSELYKY KVVQIEPLGI APTRAKRRVV 500
    EREKRAVGIG AMIFGFLGAA GSTMGAASIT LTVQARQLLS GIVQQQSNLL 550
    RAIEAQQHLL QLTVWGIKQL QARVLAVERY LKDQKFLGLW GCSGKIICTT 600
    AVPWNSTWSN KSFEEIWNNM TWIEWEREIS NYTNQIYEIL TESQNQQDRN 650
    EKDLLELDKW ASLWNWFDIT NWLWYIKIFI MIVGGLIGLR IIFAVLSIVN 700
    RVRQGYSPLS FQTPIHHQRE PDRPERIEEG GGEQGRDRSV RLVSGFLSLA 750
    WDDLRSLCLF SYHRLRDFIL IATRTVELLG HSSLKGLRRG WEGLKYLGNL 800
    LLYWGQELKI SAISLLNTTA IAVAGWTDRV IEVAQGAWRA ILHIPRRIRQ 850
    GLERTLL 857
    Length:857
    Mass (Da):97,310
    Last modified:October 17, 2006 - v1
    Checksum:iAA83C4FF189CCD0E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB253424 Genomic DNA. Translation: BAF31367.1.
    AB253425 Genomic DNA. Translation: BAF31376.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB253424 Genomic DNA. Translation: BAF31367.1 .
    AB253425 Genomic DNA. Translation: BAF31376.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3NGB X-ray 2.68 A/D/G/I 201-303 [» ]
    A/D/G/I 325-486 [» ]
    A/D/G/I 43-122 [» ]
    3SE8 X-ray 1.90 G 43-486 [» ]
    3SE9 X-ray 2.00 G 43-486 [» ]
    3TGT X-ray 1.90 A 43-486 [» ]
    3U7Y X-ray 2.45 G 201-303 [» ]
    G 325-486 [» ]
    G 43-122 [» ]
    4DKO X-ray 1.98 A/C 43-486 [» ]
    4DKP X-ray 1.80 A/C 43-486 [» ]
    4DKQ X-ray 1.89 A 43-486 [» ]
    4DKR X-ray 1.80 A/C 43-486 [» ]
    4DKU X-ray 2.49 A/B 201-303 [» ]
    A/B 325-486 [» ]
    A/B 43-122 [» ]
    4DKV X-ray 2.18 A/B 201-303 [» ]
    A/B 325-486 [» ]
    A/B 43-122 [» ]
    4DVS X-ray 2.10 A/B 201-302 [» ]
    A/B 325-486 [» ]
    A/B 43-122 [» ]
    4DVT X-ray 2.40 A/B 201-293 [» ]
    A/B 457-486 [» ]
    A/B 88-122 [» ]
    4DVV X-ray 1.94 A/B 201-303 [» ]
    A/B 325-486 [» ]
    A/B 43-122 [» ]
    4DVW X-ray 2.20 A/B 201-433 [» ]
    A/B 325-486 [» ]
    A/B 43-122 [» ]
    4DVX X-ray 2.40 A/B 201-303 [» ]
    A/B 325-486 [» ]
    A/B 43-122 [» ]
    4H8W X-ray 1.85 G 43-486 [» ]
    4I54 X-ray 2.50 A/B 43-486 [» ]
    4JB9 X-ray 2.60 G 43-486 [» ]
    4JDT X-ray 3.26 G 43-486 [» ]
    4JKP X-ray 2.82 G 43-486 [» ]
    4JPV X-ray 2.83 G 43-486 [» ]
    4JPW X-ray 2.90 G 43-486 [» ]
    4LSP X-ray 2.15 G 43-486 [» ]
    4LSQ X-ray 2.25 G 43-486 [» ]
    4LSR X-ray 2.28 G 43-486 [» ]
    4LSU X-ray 2.30 G 43-486 [» ]
    4P9H X-ray 3.00 G 201-303 [» ]
    G 325-486 [» ]
    G 43-122 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei Q0ED31.

    Family and domain databases

    Gene3Di 2.170.40.20. 2 hits.
    InterProi IPR000777. HIV1_GP160.
    IPR000328. Retroviral_envelope_protein.
    [Graphical view ]
    Pfami PF00516. GP120. 1 hit.
    PF00517. GP41. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56502. SSF56502. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Construction and characterization of HIV-1 CRF01_AE infectious molecular clones."
      Sakamoto Y., Takekawa N., Tatsumi M.
      Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: 93TH057Imported.
    2. Cited for: X-RAY CRYSTALLOGRAPHY (2.68 ANGSTROMS) OF 201-303; 325-486 AND 43-122, GLYCOSYLATION AT ASN-87; ASN-236; ASN-243; ASN-264; ASN-278; ASN-291; ASN-297; ASN-335; ASN-386; ASN-392 AND ASN-442.
    3. Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 43-486, GLYCOSYLATION AT ASN-87; ASN-160 AND ASN-188.
    4. "Increasing the potency and breadth of an HIV antibody by using structure-based rational design."
      Diskin R., Scheid J.F., Marcovecchio P.M., West A.P., Klein F., Gao H., Gnanapragasam P.N., Abadir A., Seaman M.S., Nussenzweig M.C., Bjorkman P.J.
      Science 334:1289-1293(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.45 ANGSTROMS) OF 201-303; 325-486 AND 43-122, GLYCOSYLATION AT ASN-87; ASN-236; ASN-264; ASN-291; ASN-297 AND ASN-386.
    5. "Structure-based design, synthesis, and characterization of dual hotspot small-molecule HIV-1 entry inhibitors."
      LaLonde J.M., Kwon Y.D., Jones D.M., Sun A.W., Courter J.R., Soeta T., Kobayashi T., Princiotto A.M., Wu X., Schon A., Freire E., Kwong P.D., Mascola J.R., Sodroski J., Madani N., Smith A.B.
      J. Med. Chem. 55:4382-4396(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 43-486, GLYCOSYLATION AT ASN-160 AND ASN-188.
    6. "Unliganded HIV-1 gp120 core structures assume the CD4-bound conformation with regulation by quaternary interactions and variable loops."
      Kwon Y.D., Finzi A., Wu X., Dogo-Isonagie C., Lee L.K., Moore L.R., Schmidt S.D., Stuckey J., Yang Y., Zhou T., Zhu J., Vicic D.A., Debnath A.K., Shapiro L., Bewley C.A., Mascola J.R., Sodroski J.G., Kwong P.D.
      Proc. Natl. Acad. Sci. U.S.A. 109:5663-5668(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 43-486, GLYCOSYLATION AT ASN-160 AND ASN-188.
    7. "Antiviral activity and binding mode of next generation CD4-mimetics."
      Curreli F., Kwon Y.D., Zhang H, Kwong P.D., Debnath A.K.
      Submitted (FEB-2012) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.49 ANGSTROMS) OF 201-303; 325-486 AND 43-122, GLYCOSYLATION AT ASN-236; ASN-243; ASN-264; ASN-278; ASN-291; ASN-297; ASN-335; ASN-356; ASN-386 AND ASN-442.
    8. "Structural definition of new transitional epitopes exposed by CD4 binding to HIV-1."
      Pazgier M., Acharya P., Tolbert W.D., Gohain N., Meginstu M., Wu X., Sajadi M., Robinson J., Kamin-Lewis R., Kwong P.D., Guan J., DeVico A.L., Lewis G.K.
      Submitted (SEP-2012) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) OF 43-486, GLYCOSYLATION AT ASN-188.
    9. "Structure-Based Design and Synthesis of an HIV-1 Entry Inhibitor Exploiting X-Ray and Thermodynamic Characterization."
      Lalonde J.M., Le-Khac M., Jones D.M., Courter J.R., Park J., Schon A., Princiotto A.M., Wu X., Mascola J.R., Freire E., Sodroski J., Madani N., Hendrickson W.A., Smith A.B.
      ACS Med. Chem. Lett. 4:338-343(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 43-486, GLYCOSYLATION AT ASN-160 AND ASN-188.
    10. "Somatic mutations of the immunoglobulin framework are generally required for broad and potent HIV-1 neutralization."
      Klein F., Diskin R., Scheid J.F., Gaebler C., Mouquet H., Georgiev I.S., Pancera M., Zhou T., Incesu R.B., Fu B.Z., Gnanapragasam P.N., Oliveira T.Y., Seaman M.S., Kwong P.D., Bjorkman P.J., Nussenzweig M.C.
      Cell 153:126-138(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.83 ANGSTROMS) OF 43-486, GLYCOSYLATION AT ASN-160 AND ASN-188.
    11. Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) OF 43-486, GLYCOSYLATION AT ASN-87; ASN-160 AND ASN-188.
    12. "Restricting HIV-1 pathways for escape using rationally designed anti-HIV-1 antibodies."
      Diskin R., Klein F., Horwitz J.A., Halper-Stromberg A., Sather D.N., Marcovecchio P.M., Lee T., West A.P., Gao H., Seaman M.S., Stamatatos L., Nussenzweig M.C., Bjorkman P.J.
      J. Exp. Med. 210:1235-1249(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.82 ANGSTROMS) OF 43-486, GLYCOSYLATION AT ASN-160 AND ASN-188.
    13. "Structural basis for HIV-1 gp120 recognition by a germ-line version of a broadly neutralizing antibody."
      Scharf L., West A.P., Gao H., Lee T., Scheid J.F., Nussenzweig M.C., Bjorkman P.J., Diskin R.
      Proc. Natl. Acad. Sci. U.S.A. 110:6049-6054(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.26 ANGSTROMS) OF 43-486, GLYCOSYLATION AT ASN-188.
    14. Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) OF 43-486, GLYCOSYLATION AT ASN-160 AND ASN-188.
    15. "Antibody 8ANC195 reveals a site of broad vulnerability on the HIV-1 envelope spike."
      Scharf L., Scheid J.F., Lee J.H., West A.P., Chen C., Gao H., Gnanapragasam P.N., Mares R., Seaman M.S., Ward A.B., Nussenzweig M.C., Bjorkman P.J.
      Cell Rep. 7:785-795(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.00 ANGSTROMS) OF 201-303; 325-486 AND 43-122 IN COMPLEX WITH MANNOSE, GLYCOSYLATION AT ASN-236; ASN-243; ASN-264; ASN-278; ASN-297; ASN-356 AND ASN-386.
    16. "Crystal structures of HIV-1 gp120 envelope glycoprotein in complex with NBD analogues that target the CD4-binding site."
      Kwon Y.D., LaLonde J.M., Yang Y., Elban M.A., Sugawara A., Courter J.R., Jones D.M., Smith A.B., Debnath A.K., Kwong P.D.
      PLoS ONE 9:e85940-e85940(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.94 ANGSTROMS) OF 201-303; 325-486 AND 43-122, GLYCOSYLATION AT ASN-236; ASN-243; ASN-264; ASN-278; ASN-291; ASN-297; ASN-335; ASN-356; ASN-386; ASN-392 AND ASN-442.

    Entry informationi

    Entry nameiQ0ED31_9HIV1
    AccessioniPrimary (citable) accession number: Q0ED31
    Entry historyi
    Integrated into UniProtKB/TrEMBL: October 17, 2006
    Last sequence update: October 17, 2006
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3