Reviewed, UniProtKB/Swiss-Prot Q0EAB8 (TPH2_HORSE)

Names and origin

Protein names

Recommended name:
    Tryptophan 5-hydroxylase 2
    EC=1.14.16.4
Alternative name(s):
    Tryptophan 5-monooxygenase 2

Gene names

Name:

TPH2

Organism

Equus caballus (Horse)

Taxonomic identifier

9796 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Perissodactyla › Equidae › Equus

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	L-tryptophan + tetrahydrobiopterin + O2 = 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin.
Cofactor	Fe2+ ion By similarity.
Pathway	Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.
Sequence similarities	Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. Contains 1 ACT domain.

Ontologies

Keywords
Biological process	Serotonin biosynthesis
Ligand	Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW serotonin biosynthetic process from tryptophan Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW tryptophan 5-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 491	491	Tryptophan 5-hydroxylase 2		PRO_0000260319
Regions
	Domain	65 – 137	73	ACT
Sites
	Metal binding	319	1	Iron By similarity
	Metal binding	324	1	Iron By similarity
	Metal binding	364	1	Iron By similarity
Amino acid modifications
	Modified residue	383	1	Phosphoserine By similarity
	Modified residue	393	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0EAB8-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 50DDC8CC992C752E	FASTA	491	56,087
	10 20 30 40 50 60 MQPAMMMFSS KYWARRGFSL DSAVPEEHQL LGNLTVNKSN SGKNDDKKGN KGSSRSETAP 70 80 90 100 110 120 DSGKTAVVFS LRNEVGGLVK ALKLFQEKHV NMVHIESRKS RRRSSEVEIF VDCECGKTEF 130 140 150 160 170 180 NELIQLLKFQ TTIVTLNPPE NIWTEEEELE DVPWFPRKIS ELDKCSHRVL MYGSELDADH 190 200 210 220 230 240 PGFKDNVYRQ RRKYFVDVAM SYKYGQPIPR VEYTEEETKT WGVVFRELSR LYPTHACQEY 250 260 270 280 290 300 LKNFPLLTKY CGYREDNVPQ LEDVSMFLKE RSGFAVRPVA GYLSPRDFLA GLAYRVFHCT 310 320 330 340 350 360 QYVRHSSDPL YTPEPDTCHE LLGHVPLLAD PKFAQFSQEI GLASLGASDE DVQKLATCYF 370 380 390 400 410 420 FTIEFGLCKQ EGQLRAYGAG LLSSIGELKH ALSDKACVKA FDPKTTCLQE CLITTFQEAY 430 440 450 460 470 480 FVSESFEEAK EKMREFAKSI TRPFSVHFNP YTQSVEVLKD SRSIESVVQD LRSDLNTVCD 490 ALNKMNQYLG V

References

[1]	"Equus caballus tryptophan hydroxylase 2 (TPH2), mRNA." Momozawa Y., Takeuchi Y., Mori Y. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB264323 mRNA. Translation: BAF32950.1.
RefSeq	NP_001075252.1.
UniGene	Eca.12475
3D structure databases
SMR	Q0EAB8. Positions 152-440.
ModBase	Search...
Genome annotation databases
Ensembl	ENSECAG00000013175. Equus caballus. [Contig view]
GeneID	100009684.
KEGG	ecb:100009684.
Phylogenomic databases
HOVERGEN	Q0EAB8.
OMA	Q0EAB8. MFLKERS.
Enzyme and pathway databases
BRENDA	1.14.16.4. 1464.
Family and domain databases
InterPro	IPR002912. ACT_bd. IPR001273. ArAA_hydroxylase. IPR018301. ArAA_hydroxylase_Fe/CU_BS. IPR019774. Aromatic-AA_hydroxylase_C. IPR005963. Trp_5_mOase. IPR019773. Tyrosine_3-monooxygenase-like. [Graphical view]
Gene3D	G3DSA:1.10.800.10. Aaa_hydroxylase. 1 hit.
PANTHER	PTHR11473. Aaa_hydroxylase. 1 hit.
Pfam	PF01842. ACT. 1 hit. PF00351. Biopterin_H. 1 hit. [Graphical view]
PIRSF	PIRSF000336. TH. 1 hit.
PRINTS	PR00372. FYWHYDRXLASE.
ProDom	PD002559. Aaa_hydroxylase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01270. Trp_5_monoox. 1 hit.
PROSITE	PS00367. BIOPTERIN_HYDROXYL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPH2_HORSE

Accession

Primary (citable) accession number: Q0EAB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	October 17, 2006
Last modified:	June 16, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)