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Protein

Eukaryotic translation initiation factor 3 subunit B

Gene

eIF3-S9

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome.

GO - Molecular functioni

  • mRNA binding Source: FlyBase
  • nucleotide binding Source: InterPro
  • translation initiation factor activity Source: UniProtKB

GO - Biological processi

  • formation of translation preinitiation complex Source: UniProtKB-HAMAP
  • ovarian follicle cell development Source: FlyBase
  • regulation of translational initiation Source: UniProtKB
  • translational initiation Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-DME-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-DME-72649. Translation initiation complex formation.
R-DME-72689. Formation of a pool of free 40S subunits.
R-DME-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-DME-72702. Ribosomal scanning and start codon recognition.
R-DME-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit BUniRule annotation
Short name:
eIF3bUniRule annotation
Alternative name(s):
Eukaryotic translation initiation factor 3 subunit 9UniRule annotation
Gene namesi
Name:eIF3-S9UniRule annotation
ORF Names:CG4878
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0034237. eIF3-S9.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 690690Eukaryotic translation initiation factor 3 subunit BPRO_0000363797Add
BLAST

Proteomic databases

PaxDbiQ0E940.
PRIDEiQ0E940.

Expressioni

Gene expression databases

BgeeiQ0E940.
ExpressionAtlasiQ0E940. differential.
GenevisibleiQ0E940. DM.

Interactioni

Subunit structurei

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex. The eIF-3 complex interacts with pix (PubMed:17392269). Interacts with mxt (PubMed:23716590).UniRule annotation2 Publications

Protein-protein interaction databases

BioGridi62670. 19 interactions.
IntActiQ0E940. 9 interactions.
STRINGi7227.FBpp0086098.

Structurei

3D structure databases

ProteinModelPortaliQ0E940.
SMRiQ0E940. Positions 42-145, 194-591.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini57 – 14185RRMUniRule annotationAdd
BLAST
Repeati207 – 24640WD 1Add
BLAST
Repeati293 – 33139WD 2Add
BLAST
Repeati334 – 36936WD 3Add
BLAST
Repeati442 – 48443WD 4Add
BLAST
Repeati530 – 57546WD 5Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili595 – 64551UniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the eIF-3 subunit B family.UniRule annotation
Contains 1 RRM (RNA recognition motif) domain.UniRule annotation
Contains 5 WD repeats.UniRule annotation

Keywords - Domaini

Coiled coil, Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG2314. Eukaryota.
COG5354. LUCA.
GeneTreeiENSGT00550000074913.
InParanoidiQ0E940.
KOiK03253.
OMAiDYQEEPN.
OrthoDBiEOG7KM5S6.
PhylomeDBiQ0E940.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
3.30.70.330. 1 hit.
HAMAPiMF_03001. eIF3b.
InterProiIPR011400. EIF3B.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR013979. TIF_beta_prop-like.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PANTHERiPTHR14068. PTHR14068. 1 hit.
PfamiPF08662. eIF2A. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
PIRSFiPIRSF036424. eIF3b. 1 hit.
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0E940-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKKKSEEHS GTDANDSDYQ EEPNFDDPPG FVDNISDEDL LGDMLAQRPS
60 70 80 90 100
EADGVESVVV VDNIPKVEPV RLEKLKLVIN KLFSNYGEIV NVVYPVDEEG
110 120 130 140 150
KTKGYAFMEY KQARQAEEAV KKLNNHRLDK NHTFAVNLFT DFQKYENIPE
160 170 180 190 200
KWEPPTVQTF KVQSDLYNFI NDPDTYDQYC VAAETAPNCV QVGFWQNVLP
210 220 230 240 250
EPFELETRER FTDTFVKWSP LGTYVVTFHK PGVAIWGGSS FQKIQKFPHP
260 270 280 290 300
GTQFVEFSPC ENYLVTYGPT PTGQKIIIWD IRTGAEKRSF VADGMSVLSM
310 320 330 340 350
FRWSHDDKFV ARMGENSIHI YETPSFYLLD LKSIKIPGIR GFSWSPTDNV
360 370 380 390 400
IAYWVEEQNQ IPARVTLMEI PKKREIRNKN LFHVADCKLH WQKSGDYLCV
410 420 430 440 450
KVDRYSKLKK DKKDLDVKFL GMFYNFEIFH MREKEIPVDS VEIRELILAF
460 470 480 490 500
AWEPVGNKFS IIHGETNSSN VSFYEVNKGV KPSLVKRLEK KSCTHLFWSP
510 520 530 540 550
RGQFIVMANL TMGTFEFVDS TNDYIITASP DHFRASEVEW DPTGRYVVTG
560 570 580 590 600
VSSWKVKEDT GFNMYTFQGR IIKRTILKNF VQFLWRPRPP TLLSEEKQKE
610 620 630 640 650
IKKNLKKYYA AFEQKDRLRL TRASKELLEK RSQLRETFME YRNKRIAEWA
660 670 680 690
EQKSRRIMLR GHVDTDNLET DEVDEEIVEF LVKEEVTLLE
Length:690
Mass (Da):80,441
Last modified:October 17, 2006 - v1
Checksum:iD7536C21C931D778
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti12 – 121T → A in AAM52578 (PubMed:12537569).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013599 Genomic DNA. Translation: AAF57842.1.
AY122066 mRNA. Translation: AAM52578.1.
BT072816 mRNA. Translation: ACN62419.1.
RefSeqiNP_611228.1. NM_137384.3.
NP_725691.1. NM_166239.4.
UniGeneiDm.7152.

Genome annotation databases

EnsemblMetazoaiFBtr0086941; FBpp0086097; FBgn0034237.
FBtr0086942; FBpp0086098; FBgn0034237.
GeneIDi36981.
KEGGidme:Dmel_CG4878.
UCSCiCG4878-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013599 Genomic DNA. Translation: AAF57842.1.
AY122066 mRNA. Translation: AAM52578.1.
BT072816 mRNA. Translation: ACN62419.1.
RefSeqiNP_611228.1. NM_137384.3.
NP_725691.1. NM_166239.4.
UniGeneiDm.7152.

3D structure databases

ProteinModelPortaliQ0E940.
SMRiQ0E940. Positions 42-145, 194-591.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi62670. 19 interactions.
IntActiQ0E940. 9 interactions.
STRINGi7227.FBpp0086098.

Proteomic databases

PaxDbiQ0E940.
PRIDEiQ0E940.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0086941; FBpp0086097; FBgn0034237.
FBtr0086942; FBpp0086098; FBgn0034237.
GeneIDi36981.
KEGGidme:Dmel_CG4878.
UCSCiCG4878-RA. d. melanogaster.

Organism-specific databases

CTDi36981.
FlyBaseiFBgn0034237. eIF3-S9.

Phylogenomic databases

eggNOGiKOG2314. Eukaryota.
COG5354. LUCA.
GeneTreeiENSGT00550000074913.
InParanoidiQ0E940.
KOiK03253.
OMAiDYQEEPN.
OrthoDBiEOG7KM5S6.
PhylomeDBiQ0E940.

Enzyme and pathway databases

ReactomeiR-DME-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-DME-72649. Translation initiation complex formation.
R-DME-72689. Formation of a pool of free 40S subunits.
R-DME-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-DME-72702. Ribosomal scanning and start codon recognition.
R-DME-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.

Miscellaneous databases

GenomeRNAii36981.
PROiQ0E940.

Gene expression databases

BgeeiQ0E940.
ExpressionAtlasiQ0E940. differential.
GenevisibleiQ0E940. DM.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
3.30.70.330. 1 hit.
HAMAPiMF_03001. eIF3b.
InterProiIPR011400. EIF3B.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR013979. TIF_beta_prop-like.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PANTHERiPTHR14068. PTHR14068. 1 hit.
PfamiPF08662. eIF2A. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
PIRSFiPIRSF036424. eIF3b. 1 hit.
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  2. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Testis.
  4. Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E.
    Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
  5. "The essential Drosophila ATP-binding cassette domain protein, pixie, binds the 40 S ribosome in an ATP-dependent manner and is required for translation initiation."
    Andersen D.S., Leevers S.J.
    J. Biol. Chem. 282:14752-14760(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PIX, ASSOCIATION WITH THE 40S RIBOSOME.
  6. "Mextli is a novel eukaryotic translation initiation factor 4E-binding protein that promotes translation in Drosophila melanogaster."
    Hernandez G., Miron M., Han H., Liu N., Magescas J., Tettweiler G., Frank F., Siddiqui N., Sonenberg N., Lasko P.
    Mol. Cell. Biol. 33:2854-2864(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MXT.

Entry informationi

Entry nameiEIF3B_DROME
AccessioniPrimary (citable) accession number: Q0E940
Secondary accession number(s): C0PUW0, Q8MR84
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: October 17, 2006
Last modified: July 6, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Translation initiation factors
    List of translation initiation factor entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.