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Protein

Eukaryotic translation initiation factor 3 subunit B

Gene

eIF3-S9

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.UniRule annotation

GO - Molecular functioni

  • mRNA binding Source: FlyBase
  • nucleotide binding Source: InterPro
  • translation initiation factor activity Source: UniProtKB

GO - Biological processi

  • formation of translation preinitiation complex Source: UniProtKB-HAMAP
  • ovarian follicle cell development Source: FlyBase
  • regulation of translational initiation Source: UniProtKB
  • translational initiation Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-DME-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-DME-72649. Translation initiation complex formation.
R-DME-72689. Formation of a pool of free 40S subunits.
R-DME-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-DME-72702. Ribosomal scanning and start codon recognition.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit BUniRule annotation
Short name:
eIF3bUniRule annotation
Alternative name(s):
Eukaryotic translation initiation factor 3 subunit 9UniRule annotation
Gene namesi
Name:eIF3-S9UniRule annotation
ORF Names:CG4878
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0034237. eIF3-S9.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003637971 – 690Eukaryotic translation initiation factor 3 subunit BAdd BLAST690

Proteomic databases

PaxDbiQ0E940.
PRIDEiQ0E940.

Expressioni

Gene expression databases

BgeeiFBgn0034237.
ExpressionAtlasiQ0E940. baseline.
GenevisibleiQ0E940. DM.

Interactioni

Subunit structurei

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex. The eIF-3 complex interacts with pix (PubMed:17392269). Interacts with mxt (PubMed:23716590).UniRule annotation2 Publications

Protein-protein interaction databases

BioGridi62670. 19 interactors.
IntActiQ0E940. 9 interactors.
STRINGi7227.FBpp0086098.

Structurei

3D structure databases

ProteinModelPortaliQ0E940.
SMRiQ0E940.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini57 – 141RRMUniRule annotationAdd BLAST85
Repeati207 – 246WD 1Add BLAST40
Repeati293 – 331WD 2Add BLAST39
Repeati334 – 369WD 3Add BLAST36
Repeati442 – 484WD 4Add BLAST43
Repeati530 – 575WD 5Add BLAST46

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili595 – 645UniRule annotationAdd BLAST51

Sequence similaritiesi

Belongs to the eIF-3 subunit B family.UniRule annotation
Contains 1 RRM (RNA recognition motif) domain.UniRule annotation
Contains 5 WD repeats.UniRule annotation

Keywords - Domaini

Coiled coil, Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG2314. Eukaryota.
COG5354. LUCA.
GeneTreeiENSGT00550000074913.
InParanoidiQ0E940.
KOiK03253.
OMAiAFMEYKQ.
OrthoDBiEOG091G02V5.
PhylomeDBiQ0E940.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
3.30.70.330. 1 hit.
HAMAPiMF_03001. eIF3b. 1 hit.
InterProiIPR011400. EIF3B.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR013979. TIF_beta_prop-like.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PANTHERiPTHR14068. PTHR14068. 1 hit.
PfamiPF08662. eIF2A. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
PIRSFiPIRSF036424. eIF3b. 1 hit.
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0E940-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKKKSEEHS GTDANDSDYQ EEPNFDDPPG FVDNISDEDL LGDMLAQRPS
60 70 80 90 100
EADGVESVVV VDNIPKVEPV RLEKLKLVIN KLFSNYGEIV NVVYPVDEEG
110 120 130 140 150
KTKGYAFMEY KQARQAEEAV KKLNNHRLDK NHTFAVNLFT DFQKYENIPE
160 170 180 190 200
KWEPPTVQTF KVQSDLYNFI NDPDTYDQYC VAAETAPNCV QVGFWQNVLP
210 220 230 240 250
EPFELETRER FTDTFVKWSP LGTYVVTFHK PGVAIWGGSS FQKIQKFPHP
260 270 280 290 300
GTQFVEFSPC ENYLVTYGPT PTGQKIIIWD IRTGAEKRSF VADGMSVLSM
310 320 330 340 350
FRWSHDDKFV ARMGENSIHI YETPSFYLLD LKSIKIPGIR GFSWSPTDNV
360 370 380 390 400
IAYWVEEQNQ IPARVTLMEI PKKREIRNKN LFHVADCKLH WQKSGDYLCV
410 420 430 440 450
KVDRYSKLKK DKKDLDVKFL GMFYNFEIFH MREKEIPVDS VEIRELILAF
460 470 480 490 500
AWEPVGNKFS IIHGETNSSN VSFYEVNKGV KPSLVKRLEK KSCTHLFWSP
510 520 530 540 550
RGQFIVMANL TMGTFEFVDS TNDYIITASP DHFRASEVEW DPTGRYVVTG
560 570 580 590 600
VSSWKVKEDT GFNMYTFQGR IIKRTILKNF VQFLWRPRPP TLLSEEKQKE
610 620 630 640 650
IKKNLKKYYA AFEQKDRLRL TRASKELLEK RSQLRETFME YRNKRIAEWA
660 670 680 690
EQKSRRIMLR GHVDTDNLET DEVDEEIVEF LVKEEVTLLE
Length:690
Mass (Da):80,441
Last modified:October 17, 2006 - v1
Checksum:iD7536C21C931D778
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12T → A in AAM52578 (PubMed:12537569).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013599 Genomic DNA. Translation: AAF57842.1.
AY122066 mRNA. Translation: AAM52578.1.
BT072816 mRNA. Translation: ACN62419.1.
RefSeqiNP_611228.1. NM_137384.3.
NP_725691.1. NM_166239.4.
UniGeneiDm.7152.

Genome annotation databases

EnsemblMetazoaiFBtr0086941; FBpp0086097; FBgn0034237.
FBtr0086942; FBpp0086098; FBgn0034237.
GeneIDi36981.
KEGGidme:Dmel_CG4878.
UCSCiCG4878-RA. d. melanogaster.
CG4878-RB. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013599 Genomic DNA. Translation: AAF57842.1.
AY122066 mRNA. Translation: AAM52578.1.
BT072816 mRNA. Translation: ACN62419.1.
RefSeqiNP_611228.1. NM_137384.3.
NP_725691.1. NM_166239.4.
UniGeneiDm.7152.

3D structure databases

ProteinModelPortaliQ0E940.
SMRiQ0E940.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi62670. 19 interactors.
IntActiQ0E940. 9 interactors.
STRINGi7227.FBpp0086098.

Proteomic databases

PaxDbiQ0E940.
PRIDEiQ0E940.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0086941; FBpp0086097; FBgn0034237.
FBtr0086942; FBpp0086098; FBgn0034237.
GeneIDi36981.
KEGGidme:Dmel_CG4878.
UCSCiCG4878-RA. d. melanogaster.
CG4878-RB. d. melanogaster.

Organism-specific databases

CTDi36981.
FlyBaseiFBgn0034237. eIF3-S9.

Phylogenomic databases

eggNOGiKOG2314. Eukaryota.
COG5354. LUCA.
GeneTreeiENSGT00550000074913.
InParanoidiQ0E940.
KOiK03253.
OMAiAFMEYKQ.
OrthoDBiEOG091G02V5.
PhylomeDBiQ0E940.

Enzyme and pathway databases

ReactomeiR-DME-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-DME-72649. Translation initiation complex formation.
R-DME-72689. Formation of a pool of free 40S subunits.
R-DME-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-DME-72702. Ribosomal scanning and start codon recognition.

Miscellaneous databases

GenomeRNAii36981.
PROiQ0E940.

Gene expression databases

BgeeiFBgn0034237.
ExpressionAtlasiQ0E940. baseline.
GenevisibleiQ0E940. DM.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
3.30.70.330. 1 hit.
HAMAPiMF_03001. eIF3b. 1 hit.
InterProiIPR011400. EIF3B.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR013979. TIF_beta_prop-like.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PANTHERiPTHR14068. PTHR14068. 1 hit.
PfamiPF08662. eIF2A. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
PIRSFiPIRSF036424. eIF3b. 1 hit.
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEIF3B_DROME
AccessioniPrimary (citable) accession number: Q0E940
Secondary accession number(s): C0PUW0, Q8MR84
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: October 17, 2006
Last modified: November 30, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Translation initiation factors
    List of translation initiation factor entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.