Beta-fructofuranosidase, insoluble isoenzyme 1 precursor - Oryza sativa subsp. japonica (Rice)

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Q0E0P0 (INV1_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 49. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Beta-fructofuranosidase, insoluble isoenzyme 1
EC=3.2.1.26

Alternative name(s):
Cell wall beta-fructosidase 1
Invertase 1
OsCIN1
Sucrose hydrolase 1

Gene names

Name:	CIN1
Ordered Locus Names:	Os02g0534400, LOC_Os02g33110
ORF Names:	B1136H02.14, OJ1112_G07.1

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza ›

Protein attributes

Sequence length	577 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May play a role in sucrose partitioning during seed development and in stress response.
Catalytic activity	Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.
Subcellular location	Secreted › extracellular space › apoplast Probable. Secreted › cell wall Probable. Note: Associated to the cell wall Probable.
Tissue specificity	Expressed in roots, leaves and flowers. Weakly expressed in seeds. Ref.1 Ref.2
Developmental stage	Expressed from 1 to 3 days after flowering mainly in the maternal tissues of the developing caryopsis, corresponding to the early grain filling stage. Ref.1 Ref.2 Ref.5
Induction	By sugar in excised leaves. By rice blast fungus (M.grisea) 4 hours after infection. Ref.2
Sequence similarities	Belongs to the glycosyl hydrolase 32 family.

Ontologies

Keywords
Cellular component	Apoplast Cell wall Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nectar secretion Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to karrikin Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to wounding Inferred from electronic annotation. Source: EnsemblPlants/Gramene starch metabolic process Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Cellular_component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell vacuole Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	inulinase activity Inferred from electronic annotation. Source: EnsemblPlants/Gramene levanase activity Inferred from electronic annotation. Source: EnsemblPlants/Gramene sucrose alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 22

Potential

Chain

23 – 577

555

Beta-fructofuranosidase, insoluble isoenzyme 1

PRO_0000033379

Sites

Active site

By similarity

Amino acid modifications

Glycosylation

158

N-linked (GlcNAc...) Potential

Glycosylation

183

N-linked (GlcNAc...) Potential

Glycosylation

333

N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict

428

D → N in BAB90855. Ref.1

Sequence conflict

475

K → E in BAB90855. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q0E0P0 [UniParc].

Last modified October 17, 2006. Version 1.
Checksum: 2A39D555E834529C
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FASTA

577

63,984

        10         20         30         40         50         60 
MGTRLLALAP WLLLLLLQLA GASHVVHRSL EAEQAPSSVP ASIVSPLLRT GYHFQPPMNW 

        70         80         90        100        110        120 
INDPNGPLYY KGWYHLFYQY NPKGAVWGNI VWAHSVSQDL INWIALEPAI KPDIPSDQYG 

       130        140        150        160        170        180 
CWSGSATILP DGTPAILYTG IDRPNINYQV QNIAFPKNAS DPLLREWVKP AYNPVATPEP 

       190        200        210        220        230        240 
GMNATQFRDP TTAWYADGHW RMLVGGLKGA RLGLAYLYRS RDFKTWVRAK HPLHSALTGM 

       250        260        270        280        290        300 
WECPDFFPLQ APGLQAGLDT SVPSSKYVLK NSLDLTRYDY YTVGIYNKVT ERYVPDNPAG 

       310        320        330        340        350        360 
DYHRLRYDYG NFYASKTFFD PVKHRRILLG WANESDSVTY DKAKGWAGIH AIPRKVWLDP 

       370        380        390        400        410        420 
SGKQLLQWPI EELETLRGKS VSVFDKVVKP GEHFQVTGLG TYQADVEVSL EVSGLEKAEA 

       430        440        450        460        470        480 
LDPAFGDDAE RLCGAKGADV RGGVVFGLWV LASAGLEEKT AVFFRVFKPA GHGAKPVVLM 

       490        500        510        520        530        540 
CTDPTKSSLS PDLYKPTFAG FVDTDISSGK ISLRSLIDRS VVESFGAGGK TCILSRVYPS 

       550        560        570 
MAIGDKAHLY VFNNGEADIK ISHLKAWEMK KPLMNGA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cell wall invertase in developing rice caryopsis: molecular cloning of OsCIN1 and analysis of its expression in relation to its role in grain filling." Hirose T., Takano M., Terao T. Plant Cell Physiol. 43:452-459(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: cv. Nipponbare. Tissue: Panicle.
[2]	"Molecular cloning and expression analysis of the cell-wall invertase gene family in rice (Oryza sativa L.)." Cho J.-I., Lee S.-K., Ko S., Kim H.-K., Jun S.-H., Lee Y.-H., Bhoo S.H., Lee K.-W., An G., Hahn T.-R., Jeon J.-S. Plant Cell Rep. 24:225-236(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION. Strain: cv. Nipponbare.
[3]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[5]	"Expression patterns of genes encoding carbohydrate-metabolizing enzymes and their relationship to grain filling in rice (Oryza sativa L.): comparison of caryopses located at different positions in a panicle." Ishimaru T., Hirose T., Matsuda T., Goto A., Takahashi K., Sasaki H., Terao T., Ishii R., Ohsugi R., Yamagishi T. Plant Cell Physiol. 46:620-628(2005) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB073749 mRNA. Translation: BAB90855.1. AY578158 mRNA. Translation: AAT84401.1. AP004156 Genomic DNA. Translation: BAD27793.1. AP005798 Genomic DNA. Translation: BAD29294.1. AP008208 Genomic DNA. Translation: BAF08948.1.
RefSeq	NP_001047034.1. NM_001053569.1.
UniGene	Os.9416.
3D structure databases
ProteinModelPortal	Q0E0P0.
ModBase	Search...
Protein family/group databases
CAZy	GH32. Glycoside Hydrolase Family 32.
Proteomic databases
PRIDE	Q0E0P0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os02g33110.1; LOC_Os02g33110.1; LOC_Os02g33110.
GeneID	4329561.
KEGG	dosa:Os02t0534400-01. osa:4329561.
Organism-specific databases
Gramene	Q0E0P0.
Phylogenomic databases
eggNOG	COG1621.
KO	K01193.
OMA	GHWRMLV.
ProtClustDB	CLSN2692792.
Gene expression databases
ArrayExpress	Q0E0P0.
Family and domain databases
Gene3D	2.115.10.20. 1 hit.
InterPro	IPR008985. ConA-like_lec_gl_sf. IPR001362. Glyco_hydro_32. IPR018053. Glyco_hydro_32_AS. IPR013189. Glyco_hydro_32_C. IPR013148. Glyco_hydro_32_N. IPR023296. Glyco_hydro_beta-prop. [Graphical view]
Pfam	PF08244. Glyco_hydro_32C. 1 hit. PF00251. Glyco_hydro_32N. 1 hit. [Graphical view]
SMART	SM00640. Glyco_32. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit. SSF75005. Glyco_hydro_43_beta-prop. 1 hit.
PROSITE	PS00609. GLYCOSYL_HYDROL_F32. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

INV1_ORYSJ

Accession

Primary (citable) accession number: Q0E0P0
Secondary accession number(s): Q6EU76, Q6VEF4, Q8S922

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	October 17, 2006
Last modified:	April 3, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents