Reviewed, UniProtKB/Swiss-Prot Q0DZE0 (PAL2_ORYSJ)

Names and origin

Protein names

Recommended name:
    Phenylalanine ammonia-lyase
    EC=4.3.1.24

Gene names

Name:	ZB8
Ordered Locus Names:	Os02g0627100, LOC_Os02g41680
ORF Names:	P0042D01.10, OsJ_007359

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	713 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This is a key enzyme of plant metabolism catalyzing the first reaction in the biosynthesis from L-phenylalanine of a wide variety of natural products based on the phenylpropane skeleton.
Catalytic activity	L-phenylalanine = trans-cinnamate + ammonia.
Pathway	Phenylpropanoid metabolism; cinnamic acid biosynthesis; trans-cinnamic acid from L-phenylalanine: step 1/1.
Subcellular location	Cytoplasm Probable.
Post-translational modification	Contains an active site 4-methylidene-imidazol-5-one (MIO), which is formed autocatalytically by cyclization and dehydration of residues Ala-Ser-Gly By similarity.
Sequence similarities	Belongs to the PAL/histidase family.

Ontologies

Keywords
Biological process	Phenylpropanoid metabolism
Cellular component	Cytoplasm
Molecular function	Lyase
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: InterPro biosynthetic process Inferred from electronic annotation. Source: InterPro phenylpropanoid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ammonia ligase activity Inferred from electronic annotation. Source: InterPro ammonia-lyase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 713	713	Phenylalanine ammonia-lyase		PRO_0000215402
Amino acid modifications
	Modified residue	200	1	2,3-didehydroalanine (Ser) By similarity
	Cross-link	199 ↔ 201		5-imidazolinone (Ala-Gly) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0DZE0-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 774C6D2B9B77348A	FASTA	713	76,981
	10 20 30 40 50 60 MECENGRVSA NGMSGLCVAA PRADPLNWGK ATEEMTGSHL DEVKRMVAEY RQPLVKIEGA 70 80 90 100 110 120 SLRIAQVAAV AAAGEARVEL DESARERVKA SSDWVMNSMM NGTDSYGVTT GFGATSHRRT 130 140 150 160 170 180 KEGGALQREL IRFLNAGAFG TGTDGHVLPA EATRAAMLVR INTLLQGYSG IRFEILEAIA 190 200 210 220 230 240 KLLNANVTPC LPLRGTITAS GDLVPLSYIA GLVTGRENAV AVAPDGSKVN AAEAFKIAGI 250 260 270 280 290 300 QGGFFELQPK EGLAMVNGTA VGSGLASTVL FEANILAILA EVLSAVFCEV MNGKPEYTDH 310 320 330 340 350 360 LTHKLKHHPG QIEAAAIMEH ILEGSSYMKH AKKLGELDPL MKPKQDRYAL RTSPQWLGPQ 370 380 390 400 410 420 IEVIRAATKS IEREINSVND NPLIDVSRGK ALHGGNFQGT PIGVSMDNTR LAIAAIGKLM 430 440 450 460 470 480 FAQFSELVND FYNNGLPSNL SGGRNPSLDY GFKGAEIAMA SYCSELQFLG NPVTNHVQSA 490 500 510 520 530 540 EQHNQDVNSL GLISSRKTDE AIDILKLMSS TFLIALCQAV DLRHIEENVK SAVKSCVMTV 550 560 570 580 590 600 AKKTLSTNST GDLHVARFCE KDLLKEIDRE AVFAYADDPC SHNYPLMKKL RNVLVERALA 610 620 630 640 650 660 NGAAEFNADT SVFAKVAQFE EELRATLPGA IEAARAAVEN GTAAIPSRIT ECRSYPLYRF 670 680 690 700 710 VREELGTKYL TGEKTRSPGE ELNKVLVAIN EGKHIDPLLE CLKEWNGEPL PIC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[3]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP005000 Genomic DNA. Translation: BAD23155.1. AP008208 Genomic DNA. Translation: BAF09398.1. CM000139 Genomic DNA. Translation: EAZ23876.1. AK068993 mRNA. No translation available.
RefSeq	NP_001047484.1.
UniGene	Os.25687
3D structure databases
HSSP	HSSP built from PDB template 1GKM based on UniProtKB P21310.
SMR	Q0DZE0. Positions 24-713.
ModBase	Search...
Genome annotation databases
GeneID	4330040.
KEGG	osa:4330040.
Organism-specific databases
Gramene	P53443.
Family and domain databases
InterPro	IPR001106. Phe/His_NH3-lyase. IPR005922. Phe_NH3-lyase. [Graphical view]
Pfam	PF00221. PAL. 1 hit. [Graphical view]
TIGRFAMs	TIGR01226. phe_am_lyase. 1 hit.
PROSITE	PS00488. PAL_HISTIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PAL2_ORYSJ

Accession

Primary (citable) accession number: Q0DZE0
Secondary accession number(s): P53443, Q6K6P7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	October 17, 2006
Last modified:	June 16, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)