Q0DY59 (HMDH1_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-hydroxy-3-methylglutaryl-coenzyme A reductase 1
Short name=HMG-CoA reductase 1
EC=1.1.1.34

Gene names

Name:	HMG1
Ordered Locus Names:	Os02g0713900, LOC_Os02g48330

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	532 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the synthesis of mevalonate. The specific precursor of all isoprenoid compounds present in plants.
Catalytic activity	(R)-mevalonate + CoA + 2 NADP+ = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
Pathway	Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein.
Sequence similarities	Belongs to the HMG-CoA reductase family.
Sequence caution	The sequence BAF09829.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	coenzyme A metabolic process Inferred from electronic annotation. Source: InterPro isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro hydroxymethylglutaryl-CoA reductase (NADPH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 532	532	3-hydroxy-3-methylglutaryl-coenzyme A reductase 1		PRO_0000114446
Regions
	Transmembrane	63 – 83	21	Helical; Potential
	Region	77 – 117	41	Linker By similarity
	Region	118 – 532	415	Catalytic By similarity
Sites
	Active site	211	1	Charge relay system By similarity
	Active site	343	1	Charge relay system By similarity
	Active site	419	1	Charge relay system By similarity
	Active site	517	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	275	1	N-linked (GlcNAc...) Potential
	Glycosylation	521	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q0DY59 [UniParc]. Last modified July 24, 2007. Version 2. Checksum: D70406C15BA50127	FASTA	532	55,615
	10 20 30 40 50 60 MDVRRGGGGG RIVGAARRAL TWGALPLPMR ITNGLAMVSL VLSSCDLLRL CSDRERPLGG 70 80 90 100 110 120 REFATVVYLV SLFAHPDAPA TTTGDDDDGQ GGSRRARPAA AEPAPMHGHG GGMMEADDEE 130 140 150 160 170 180 IVAAVASGAL PSHRLESRLG DCRRAARLRR EALRRVTGRG VEGLPFDGMD YQAILGQCCE 190 200 210 220 230 240 MPVGYVQLPV GVAGPLLLDG REYHVPMATT EGCLVASVNR GCRAISASGG AFSVLLRDAM 250 260 270 280 290 300 SRAPAVKLPS AMRAAELKAF AEAPANFELL AAVFNRSSRF GRLQDIRCAL AGRNLYMRFS 310 320 330 340 350 360 CITGDAMGMN MVSKGVENVL GYLQNVFPDM DVISVSGNYC SDKKPTAVNW IEGRGKSVVC 370 380 390 400 410 420 EAIIKGDVVQ KVLKTTVEKL VELNIIKNLA GSAVAGALGG FNAHASNIVT ALFIATGQDP 430 440 450 460 470 480 AQNVESSQCI TMLEEVNDGD DLHISVTMPS IEVGTIGGGT CLASQAACLN LLGVKGSNHG 490 500 510 520 530 SPGANAKRLA TIVAGSVLAG ELSLLAALAS GHLVKSHMMY NRSSKDVAKA AS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed: 18089549] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008208 Genomic DNA. Translation: BAF09829.1. Sequence problems.
3D structure databases
ProteinModelPortal	Q0DY59.
SMR	Q0DY59. Positions 115-514.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	P48019.
Family and domain databases
InterPro	IPR002202. HMG_CoA_Rdtase. IPR023074. HMG_CoA_Rdtase_cat. IPR023076. HMG_CoA_Rdtase_CS. IPR004554. HMG_CoA_Rdtase_eu_arc. IPR023282. HMG_CoA_Rdtase_N. IPR009023. HMG_CoA_Rdtase_NAD(P)-bd. IPR009029. HMG_CoA_Rdtase_sub-bd. [Graphical view]
Gene3D	G3DSA:3.30.70.420. G3DSA:3.30.70.420. 1 hit. G3DSA:3.90.770.10. HMG-CoA_red. 2 hits. G3DSA:1.10.3270.10. HMG_CoA_Rdtase_N. 1 hit.
PANTHER	PTHR10572. HMG-CoA_red. 1 hit.
Pfam	PF00368. HMG-CoA_red. 1 hit. [Graphical view]
PRINTS	PR00071. HMGCOARDTASE.
SUPFAM	SSF55035. HMG_CoA_NAD_bind. 1 hit. SSF56542. HMG_CoA_sub_bind. 1 hit.
TIGRFAMs	TIGR00533. HMG_CoA_R_NADP. 1 hit.
PROSITE	PS00066. HMG_COA_REDUCTASE_1. 1 hit. PS00318. HMG_COA_REDUCTASE_2. 1 hit. PS01192. HMG_COA_REDUCTASE_3. 1 hit. PS50065. HMG_COA_REDUCTASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HMDH1_ORYSJ

Accession

Primary (citable) accession number: Q0DY59
Secondary accession number(s): P48019

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	July 24, 2007
Last modified:	June 28, 2011
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program