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Q0DM48 (MAN3_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mannan endo-1,4-beta-mannosidase 3

EC=3.2.1.78
Alternative name(s):
Beta-mannanase 3
Endo-beta-1,4-mannanase 3
OsMAN3
Gene names
Name:MAN3
Ordered Locus Names:Os03g0828300, LOC_Os03g61270
ORF Names:OSJNBa0010E04.20
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length468 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.

Subcellular location

Secreted Potential.

Tissue specificity

Expressed in seeds. Ref.4

Sequence similarities

Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Sequence caution

The sequence ABF99675.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence BAF13690.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmannan endo-1,4-beta-mannosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 468445Mannan endo-1,4-beta-mannosidase 3
PRO_0000277484

Sites

Active site2021Proton donor By similarity
Active site3211Nucleophile By similarity

Amino acid modifications

Glycosylation1521N-linked (GlcNAc...) Potential
Glycosylation3001N-linked (GlcNAc...) Potential
Glycosylation3331N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q0DM48 [UniParc].

Last modified February 6, 2007. Version 2.
Checksum: 2AD51477F32EAD11

FASTA46851,217
        10         20         30         40         50         60 
MTVRPRPAAA AIIIAAVFGA AAAAAGGGMV GVDGTQFVVE GGRTIYFSGF NAYWLMMMAS 

        70         80         90        100        110        120 
DPARRAAVVA AFAQASSRGL NLARTWAFSD GGDQPLQSSP GVYDEAMFQG LDFVIAEARR 

       130        140        150        160        170        180 
HGIYLLLCLT NNFDDFGGKR QYVRWAADAG HNLTAGDDFF TSSVVKSYYK NHVKAVLTRV 

       190        200        210        220        230        240 
NTVTGVAYKD DPTIFAWELM NEPRCDADPT GGMVQAWVEE MAPYVKRVDG GRHLVTAGLE 

       250        260        270        280        290        300 
GFYGDGEHES KELNPWGIYY GTNYVATHRA AGVDFATIHL YPDVWLWGST ADEQAAFFRN 

       310        320        330        340        350        360 
WTRSHVHDTA AFLGKPLLVT EYGKFLWKGG GANKTQRNYF LDVVLDAIYA SASRGGPLVG 

       370        380        390        400        410        420 
GAFWQLLLDD DVVAGMDDLR DGYEIILAED SRAASIIGEH SEQLASLNGQ DAEALRRRRR 

       430        440        450        460 
RPASSHRKTR LGSGGDSDAL RLPRTLLIRF ISLSRSISSF IQDNFVLF 

« Hide

References

« Hide 'large scale' references
[1]"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
The rice chromosome 3 sequencing consortium
Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. expand/collapse author list , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[2]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"The rice annotation project database (RAP-DB): 2008 update."
The rice annotation project (RAP)
Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[4]"The endo-beta-mannanase gene families in Arabidopsis, rice, and poplar."
Yuan J.S., Yang X., Lai J., Lin H., Cheng Z.-M., Nonogaki H., Chen F.
Funct. Integr. Genomics 7:1-16(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC096687 Genomic DNA. Translation: AAL79761.1.
DP000009 Genomic DNA. Translation: ABF99675.1. Sequence problems.
AP008209 Genomic DNA. Translation: BAF13690.1. Sequence problems.

3D structure databases

ProteinModelPortalQ0DM48.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneQ0DM48.

Phylogenomic databases

eggNOGCOG3934.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMAN3_ORYSJ
AccessionPrimary (citable) accession number: Q0DM48
Secondary accession number(s): Q10B69, Q8SAY1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: February 6, 2007
Last modified: February 19, 2014
This is version 44 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries