Q0DM48 (MAN3_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mannan endo-1,4-beta-mannosidase 3
EC=3.2.1.78

Alternative name(s):
Beta-mannanase 3
Endo-beta-1,4-mannanase 3
OsMAN3

Gene names

Name:	MAN3
Ordered Locus Names:	Os03g0828300, LOC_Os03g61270
ORF Names:	OSJNBa0010E04.20

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.
Subcellular location	Secreted Potential.
Tissue specificity	Expressed in seeds. Ref.4
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family.
Sequence caution	The sequence ABF99675.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAF13690.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	mannan endo-1,4-beta-mannosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 468	445	Mannan endo-1,4-beta-mannosidase 3		PRO_0000277484
Sites
	Active site	202	1	Proton donor By similarity
	Active site	321	1	Nucleophile By similarity
Amino acid modifications
	Glycosylation	152	1	N-linked (GlcNAc...) Potential
	Glycosylation	300	1	N-linked (GlcNAc...) Potential
	Glycosylation	333	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q0DM48 [UniParc]. Last modified February 6, 2007. Version 2. Checksum: 2AD51477F32EAD11	FASTA	468	51,217
	10 20 30 40 50 60 MTVRPRPAAA AIIIAAVFGA AAAAAGGGMV GVDGTQFVVE GGRTIYFSGF NAYWLMMMAS 70 80 90 100 110 120 DPARRAAVVA AFAQASSRGL NLARTWAFSD GGDQPLQSSP GVYDEAMFQG LDFVIAEARR 130 140 150 160 170 180 HGIYLLLCLT NNFDDFGGKR QYVRWAADAG HNLTAGDDFF TSSVVKSYYK NHVKAVLTRV 190 200 210 220 230 240 NTVTGVAYKD DPTIFAWELM NEPRCDADPT GGMVQAWVEE MAPYVKRVDG GRHLVTAGLE 250 260 270 280 290 300 GFYGDGEHES KELNPWGIYY GTNYVATHRA AGVDFATIHL YPDVWLWGST ADEQAAFFRN 310 320 330 340 350 360 WTRSHVHDTA AFLGKPLLVT EYGKFLWKGG GANKTQRNYF LDVVLDAIYA SASRGGPLVG 370 380 390 400 410 420 GAFWQLLLDD DVVAGMDDLR DGYEIILAED SRAASIIGEH SEQLASLNGQ DAEALRRRRR 430 440 450 460 RPASSHRKTR LGSGGDSDAL RLPRTLLIRF ISLSRSISSF IQDNFVLF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species." The rice chromosome 3 sequencing consortium Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S. Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"The endo-beta-mannanase gene families in Arabidopsis, rice, and poplar." Yuan J.S., Yang X., Lai J., Lin H., Cheng Z.-M., Nonogaki H., Chen F. Funct. Integr. Genomics 7:1-16(2007) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC096687 Genomic DNA. Translation: AAL79761.1. DP000009 Genomic DNA. Translation: ABF99675.1. Sequence problems. AP008209 Genomic DNA. Translation: BAF13690.1. Sequence problems.
3D structure databases
ProteinModelPortal	Q0DM48.
ModBase	Search...
Protein family/group databases
CAZy	GH5. Glycoside Hydrolase Family 5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
KEGG	dosa:Os03t0828300-01.
Organism-specific databases
Gramene	Q0DM48.
Phylogenomic databases
eggNOG	COG3934.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR001547. Glyco_hydro_5. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00150. Cellulase. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00659. GLYCOSYL_HYDROL_F5. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAN3_ORYSJ

Accession

Primary (citable) accession number: Q0DM48
Secondary accession number(s): Q10B69, Q8SAY1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	February 6, 2007
Last modified:	May 1, 2013
This is version 42 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program