Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Guanine nucleotide-binding protein alpha-1 subunit

Gene

GPA1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems (Probable). May function in a signal transduction pathway required for normal growth and development of internodes, leaves, panicles and seeds (PubMed:10377457). Involved in gibberellin signal transduction (PubMed:10468600, PubMed:11027362). Involved in R gene-mediated disease resistance. Functions upstream of the small GTPase RAC1 in the early steps of signaling (PubMed:12237405). Involved in brassinosteroid response. May not be a signaling molecule in BRI1-meditated perception or transduction (PubMed:17117160, PubMed:19036785).Curated6 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi53 – 531MagnesiumBy similarity
Metal bindingi194 – 1941MagnesiumBy similarity
Binding sitei356 – 3561GTP; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi46 – 538GTPBy similarity
Nucleotide bindingi188 – 1947GTPBy similarity
Nucleotide bindingi219 – 2235GTPBy similarity
Nucleotide bindingi288 – 2914GTPBy similarity

GO - Molecular functioni

  1. channel regulator activity Source: GO_Central
  2. G-protein beta/gamma-subunit complex binding Source: GO_Central
  3. G-protein coupled receptor binding Source: GO_Central
  4. GTPase activity Source: GO_Central
  5. GTPase inhibitor activity Source: GO_Central
  6. GTP binding Source: UniProtKB-KW
  7. metal ion binding Source: UniProtKB-KW
  8. signal transducer activity Source: GO_Central

GO - Biological processi

  1. abscisic acid-activated signaling pathway Source: EnsemblPlants/Gramene
  2. adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: GO_Central
  3. blue light signaling pathway Source: EnsemblPlants/Gramene
  4. cell death Source: EnsemblPlants/Gramene
  5. ethylene-activated signaling pathway Source: EnsemblPlants/Gramene
  6. gibberellic acid mediated signaling pathway Source: EnsemblPlants/Gramene
  7. G-protein coupled receptor signaling pathway, coupled to S1P second messenger Source: EnsemblPlants/Gramene
  8. L-phenylalanine biosynthetic process Source: EnsemblPlants/Gramene
  9. mitotic cell cycle Source: EnsemblPlants/Gramene
  10. negative regulation of abscisic acid-activated signaling pathway Source: EnsemblPlants/Gramene
  11. negative regulation of catalytic activity Source: GOC
  12. positive regulation of abscisic acid-activated signaling pathway Source: EnsemblPlants/Gramene
  13. reactive oxygen species metabolic process Source: EnsemblPlants/Gramene
  14. regulation of cell proliferation Source: EnsemblPlants/Gramene
  15. regulation of stomatal movement Source: EnsemblPlants/Gramene
  16. response to fructose Source: EnsemblPlants/Gramene
  17. response to glucose Source: EnsemblPlants/Gramene
  18. response to low fluence blue light stimulus by blue low-fluence system Source: EnsemblPlants/Gramene
  19. response to sucrose Source: EnsemblPlants/Gramene
  20. response to superoxide Source: EnsemblPlants/Gramene
  21. RNA processing Source: EnsemblPlants/Gramene
  22. salicylic acid mediated signaling pathway Source: EnsemblPlants/Gramene
  23. seed germination Source: EnsemblPlants/Gramene
  24. thylakoid membrane organization Source: EnsemblPlants/Gramene
  25. tyrosine biosynthetic process Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Keywords - Biological processi

Plant defense

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_285691. G alpha (i) signalling events.
REACT_293226. Inactivation, recovery and regulation of the phototransduction cascade.
REACT_312900. G alpha (z) signalling events.
REACT_317829. G-protein activation.
REACT_324068. PLC beta mediated events.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein alpha-1 subunit
Short name:
GP-alpha-1
Alternative name(s):
Protein Daikoku dwarf1 Publication
Protein Dwarf1
Gene namesi
Name:GPA1Curated
Synonyms:D11 Publication, GA1, RGA11 Publication
Ordered Locus Names:Os05g0333200Imported, LOC_Os05g26890Curated
ORF Names:OJ1005_D04.15, OSJNBa0049D13.1
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763 Componenti: Chromosome 5

Organism-specific databases

GrameneiQ0DJ33.

Subcellular locationi

  1. Cell membrane 1 Publication

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: EnsemblPlants/Gramene
  2. heterotrimeric G-protein complex Source: GO_Central
  3. plasmodesma Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Dwarf plants and small seeds (PubMed:10377457, PubMed:10468600). Short internodes, leaves and panicles (PubMed:10377457).2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi223 – 2231Q → L: Constitutively active. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 380379Guanine nucleotide-binding protein alpha-1 subunitPRO_0000203622Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity
Lipidationi5 – 51S-palmitoyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Myristate, Palmitate

Expressioni

Tissue specificityi

Highly expressed in young internodes. Expressed in the base of the stamen, pistil, lemma, and palea before flowering and in the pericarp of the ovary after fertilization.1 Publication

Inductioni

By infection with an avirulent race of rice blast fungus (M.grisea) and sphingolipid elicitors (PubMed:12237405). Down-regulated by a virulent race of rice blast fungus (M.grisea) (PubMed:12237405). Down-regulated by 24-epi-brassinolide (PubMed:17117160).2 Publications

Gene expression databases

ExpressionAtlasiQ0DJ33. baseline.

Interactioni

Subunit structurei

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site (PubMed:15078334). Interacts with COLD1 (PubMed:25728666).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
LOC_Os03g46650Q406872EBI-1100098,EBI-1100119

Protein-protein interaction databases

IntActiQ0DJ33. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ0DJ33.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The helical domain (69-189) is required for self-activation.By similarity

Sequence similaritiesi

Belongs to the G-alpha family.Curated

Phylogenomic databases

eggNOGiNOG322962.
InParanoidiQ0DJ33.
KOiK04640.

Family and domain databases

Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
IPR002976. Plant_Gprotein_alpha.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR01242. GPROTEINAPLT.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0DJ33-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSSCSRSHS LSEAETTKNA KSADIDRRIL QETKAEQHIH KLLLLGAGES
60 70 80 90 100
GKSTIFKQIK LLFQTGFDEA ELRSYTSVIH ANVYQTIKIL YEGAKELSQV
110 120 130 140 150
ESDSSKYVIS PDNQEIGEKL SDIDGRLDYP LLNKELVLDV KRLWQDPAIQ
160 170 180 190 200
ETYLRGSILQ LPDCAQYFME NLDRLAEAGY VPTKEDVLYA RVRTNGVVQI
210 220 230 240 250
QFSPVGENKR GGEVYRLYDV GGQRNERRKW IHLFEGVNAV IFCAAISEYD
260 270 280 290 300
QMLFEDETKN RMMETKELFD WVLKQRCFEK TSFILFLNKF DIFEKKIQKV
310 320 330 340 350
PLSVCEWFKD YQPIAPGKQE VEHAYEFVKK KFEELYFQSS KPDRVDRVFK
360 370 380
IYRTTALDQK LVKKTFKLID ESMRRSREGT
Length:380
Mass (Da):44,206
Last modified:July 24, 2007 - v2
Checksum:i4EEFAEDAB4463612
GO

Sequence cautioni

The sequence BAF17140.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38232 mRNA. Translation: BAA07405.1.
AC117264 Genomic DNA. Translation: AAV43839.1.
AC144739 Genomic DNA. Translation: AAW57778.2.
AP008211 Genomic DNA. Translation: BAF17140.1. Different initiation.
RefSeqiNP_001055226.1. NM_001061761.1.
UniGeneiOs.5302.

Genome annotation databases

GeneIDi4338448.
KEGGiosa:4338448.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38232 mRNA. Translation: BAA07405.1.
AC117264 Genomic DNA. Translation: AAV43839.1.
AC144739 Genomic DNA. Translation: AAW57778.2.
AP008211 Genomic DNA. Translation: BAF17140.1. Different initiation.
RefSeqiNP_001055226.1. NM_001061761.1.
UniGeneiOs.5302.

3D structure databases

ProteinModelPortaliQ0DJ33.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ0DJ33. 1 interaction.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi4338448.
KEGGiosa:4338448.

Organism-specific databases

GrameneiQ0DJ33.

Phylogenomic databases

eggNOGiNOG322962.
InParanoidiQ0DJ33.
KOiK04640.

Enzyme and pathway databases

ReactomeiREACT_285691. G alpha (i) signalling events.
REACT_293226. Inactivation, recovery and regulation of the phototransduction cascade.
REACT_312900. G alpha (z) signalling events.
REACT_317829. G-protein activation.
REACT_324068. PLC beta mediated events.

Gene expression databases

ExpressionAtlasiQ0DJ33. baseline.

Family and domain databases

Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
IPR002976. Plant_Gprotein_alpha.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR01242. GPROTEINAPLT.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of a cDNA for the alpha subunit of a G protein from rice."
    Ishikawa A., Tsubouchi H., Iwasaki Y., Asahi T.
    Plant Cell Physiol. 36:353-359(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Nipponbare.
    Tissue: Leaf.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  4. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  5. "Suppression of the heterotrimeric G protein causes abnormal morphology, including dwarfism, in rice."
    Fujisawa Y., Kato T., Ohki S., Ishikawa A., Kitano H., Sasaki T., Asahi T., Iwasaki Y.
    Proc. Natl. Acad. Sci. U.S.A. 96:7575-7580(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  6. "Rice gibberellin-insensitive dwarf mutant gene Dwarf 1 encodes the alpha-subunit of GTP-binding protein."
    Ashikari M., Wu J., Yano M., Sasaki T., Yoshimura A.
    Proc. Natl. Acad. Sci. U.S.A. 96:10284-10289(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  7. "Rice dwarf mutant d1, which is defective in the alpha subunit of the heterotrimeric G protein, affects gibberellin signal transduction."
    Ueguchi-Tanaka M., Fujisawa Y., Kobayashi M., Ashikari M., Iwasaki Y., Kitano H., Matsuoka M.
    Proc. Natl. Acad. Sci. U.S.A. 97:11638-11643(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "The heterotrimeric G protein alpha subunit acts upstream of the small GTPase Rac in disease resistance of rice."
    Suharsono U., Fujisawa Y., Kawasaki T., Iwasaki Y., Satoh H., Shimamoto K.
    Proc. Natl. Acad. Sci. U.S.A. 99:13307-13312(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.
  9. "Characterization of heterotrimeric G protein complexes in rice plasma membrane."
    Kato C., Mizutani T., Tamaki H., Kumagai H., Kamiya T., Hirobe A., Fujisawa Y., Kato H., Iwasaki Y.
    Plant J. 38:320-331(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, SUBCELLULAR LOCATION, MUTAGENESIS OF GLN-223.
  10. "Heterotrimeric G protein alpha subunit is involved in rice brassinosteroid response."
    Wang L., Xu Y.Y., Ma Q.B., Li D., Xu Z.H., Chong K.
    Cell Res. 16:916-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.
  11. "Function of the alpha subunit of rice heterotrimeric G protein in brassinosteroid signaling."
    Oki K., Inaba N., Kitagawa K., Fujioka S., Kitano H., Fujisawa Y., Kato H., Iwasaki Y.
    Plant Cell Physiol. 50:161-172(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. Cited for: INTERACTION WITH COLD1.
    Strain: cv. Dongjin and cv. Nipponbare.

Entry informationi

Entry nameiGPA1_ORYSJ
AccessioniPrimary (citable) accession number: Q0DJ33
Secondary accession number(s): P49083
, Q2HNY6, Q43604, Q5KQF9, Q5W732
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: July 24, 2007
Last modified: April 29, 2015
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.