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Q0DJ33

- GPA1_ORYSJ

UniProt

Q0DJ33 - GPA1_ORYSJ

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Protein

Guanine nucleotide-binding protein alpha-1 subunit

Gene

GPA1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi53 – 531MagnesiumBy similarity
Metal bindingi194 – 1941MagnesiumBy similarity
Binding sitei356 – 3561GTP; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi46 – 538GTPBy similarity
Nucleotide bindingi188 – 1947GTPBy similarity
Nucleotide bindingi219 – 2235GTPBy similarity
Nucleotide bindingi288 – 2914GTPBy similarity

GO - Molecular functioni

  1. channel regulator activity Source: RefGenome
  2. G-protein beta/gamma-subunit complex binding Source: RefGenome
  3. G-protein coupled receptor binding Source: RefGenome
  4. GTPase activity Source: RefGenome
  5. GTPase inhibitor activity Source: RefGenome
  6. GTP binding Source: UniProtKB-KW
  7. metal ion binding Source: UniProtKB-KW
  8. signal transducer activity Source: RefGenome

GO - Biological processi

  1. abscisic acid-activated signaling pathway Source: EnsemblPlants/Gramene
  2. adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: RefGenome
  3. blue light signaling pathway Source: EnsemblPlants/Gramene
  4. cell death Source: EnsemblPlants/Gramene
  5. ethylene-activated signaling pathway Source: EnsemblPlants/Gramene
  6. gibberellic acid mediated signaling pathway Source: EnsemblPlants/Gramene
  7. G-protein coupled receptor signaling pathway, coupled to S1P second messenger Source: EnsemblPlants/Gramene
  8. L-phenylalanine biosynthetic process Source: EnsemblPlants/Gramene
  9. mitotic cell cycle Source: EnsemblPlants/Gramene
  10. negative regulation of abscisic acid-activated signaling pathway Source: EnsemblPlants/Gramene
  11. negative regulation of catalytic activity Source: GOC
  12. positive regulation of abscisic acid-activated signaling pathway Source: EnsemblPlants/Gramene
  13. reactive oxygen species metabolic process Source: EnsemblPlants/Gramene
  14. regulation of cell proliferation Source: EnsemblPlants/Gramene
  15. regulation of stomatal movement Source: EnsemblPlants/Gramene
  16. response to fructose Source: EnsemblPlants/Gramene
  17. response to glucose Source: EnsemblPlants/Gramene
  18. response to low fluence blue light stimulus by blue low-fluence system Source: EnsemblPlants/Gramene
  19. response to sucrose Source: EnsemblPlants/Gramene
  20. response to superoxide Source: EnsemblPlants/Gramene
  21. RNA processing Source: EnsemblPlants/Gramene
  22. salicylic acid mediated signaling pathway Source: EnsemblPlants/Gramene
  23. seed germination Source: EnsemblPlants/Gramene
  24. thylakoid membrane organization Source: EnsemblPlants/Gramene
  25. tyrosine biosynthetic process Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_233194. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.
REACT_237563. Acetylcholine regulates insulin secretion.
REACT_246838. G alpha (s) signalling events.
REACT_246843. G alpha (q) signalling events.
REACT_249448. G alpha (12/13) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein alpha-1 subunit
Short name:
GP-alpha-1
Alternative name(s):
Protein Dwarf1
Gene namesi
Name:GPA1
Synonyms:D1, GA1, RGA1
Ordered Locus Names:Os05g0333200, LOC_Os05g26890
ORF Names:OJ1005_D04.15, OSJNBa0049D13.1
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 5

Organism-specific databases

GrameneiQ0DJ33.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: EnsemblPlants/Gramene
  2. heterotrimeric G-protein complex Source: RefGenome
  3. plasmodesma Source: EnsemblPlants/Gramene
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 380379Guanine nucleotide-binding protein alpha-1 subunitPRO_0000203622Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity
Lipidationi5 – 51S-palmitoyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Myristate, Palmitate

Expressioni

Gene expression databases

ExpressionAtlasiQ0DJ33. baseline.

Interactioni

Subunit structurei

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.

Binary interactionsi

WithEntry#Exp.IntActNotes
LOC_Os03g46650Q406872EBI-1100098,EBI-1100119

Protein-protein interaction databases

IntActiQ0DJ33. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ0DJ33.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The helical domain (69-189) is required for self-activation.By similarity

Sequence similaritiesi

Belongs to the G-alpha family.Curated

Phylogenomic databases

eggNOGiNOG322962.
InParanoidiQ0DJ33.
KOiK04640.

Family and domain databases

Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
IPR002976. Plant_Gprotein_alpha.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR01242. GPROTEINAPLT.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0DJ33-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGSSCSRSHS LSEAETTKNA KSADIDRRIL QETKAEQHIH KLLLLGAGES
60 70 80 90 100
GKSTIFKQIK LLFQTGFDEA ELRSYTSVIH ANVYQTIKIL YEGAKELSQV
110 120 130 140 150
ESDSSKYVIS PDNQEIGEKL SDIDGRLDYP LLNKELVLDV KRLWQDPAIQ
160 170 180 190 200
ETYLRGSILQ LPDCAQYFME NLDRLAEAGY VPTKEDVLYA RVRTNGVVQI
210 220 230 240 250
QFSPVGENKR GGEVYRLYDV GGQRNERRKW IHLFEGVNAV IFCAAISEYD
260 270 280 290 300
QMLFEDETKN RMMETKELFD WVLKQRCFEK TSFILFLNKF DIFEKKIQKV
310 320 330 340 350
PLSVCEWFKD YQPIAPGKQE VEHAYEFVKK KFEELYFQSS KPDRVDRVFK
360 370 380
IYRTTALDQK LVKKTFKLID ESMRRSREGT
Length:380
Mass (Da):44,206
Last modified:July 24, 2007 - v2
Checksum:i4EEFAEDAB4463612
GO

Sequence cautioni

The sequence BAF17140.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38232 mRNA. Translation: BAA07405.1.
AC117264 Genomic DNA. Translation: AAV43839.1.
AC144739 Genomic DNA. Translation: AAW57778.2.
AP008211 Genomic DNA. Translation: BAF17140.1. Different initiation.
RefSeqiNP_001055226.1. NM_001061761.1.
UniGeneiOs.5302.

Genome annotation databases

GeneIDi4338448.
KEGGiosa:4338448.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38232 mRNA. Translation: BAA07405.1 .
AC117264 Genomic DNA. Translation: AAV43839.1 .
AC144739 Genomic DNA. Translation: AAW57778.2 .
AP008211 Genomic DNA. Translation: BAF17140.1 . Different initiation.
RefSeqi NP_001055226.1. NM_001061761.1.
UniGenei Os.5302.

3D structure databases

ProteinModelPortali Q0DJ33.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q0DJ33. 1 interaction.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 4338448.
KEGGi osa:4338448.

Organism-specific databases

Gramenei Q0DJ33.

Phylogenomic databases

eggNOGi NOG322962.
InParanoidi Q0DJ33.
KOi K04640.

Enzyme and pathway databases

Reactomei REACT_233194. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.
REACT_237563. Acetylcholine regulates insulin secretion.
REACT_246838. G alpha (s) signalling events.
REACT_246843. G alpha (q) signalling events.
REACT_249448. G alpha (12/13) signalling events.

Gene expression databases

ExpressionAtlasi Q0DJ33. baseline.

Family and domain databases

Gene3Di 1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProi IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
IPR002976. Plant_Gprotein_alpha.
[Graphical view ]
PANTHERi PTHR10218. PTHR10218. 1 hit.
Pfami PF00503. G-alpha. 1 hit.
[Graphical view ]
PRINTSi PR00318. GPROTEINA.
PR01242. GPROTEINAPLT.
SMARTi SM00275. G_alpha. 1 hit.
[Graphical view ]
SUPFAMi SSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of a cDNA for the alpha subunit of a G protein from rice."
    Ishikawa A., Tsubouchi H., Iwasaki Y., Asahi T.
    Plant Cell Physiol. 36:353-359(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Nipponbare.
    Tissue: Leaf.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  4. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.

Entry informationi

Entry nameiGPA1_ORYSJ
AccessioniPrimary (citable) accession number: Q0DJ33
Secondary accession number(s): P49083
, Q2HNY6, Q43604, Q5KQF9, Q5W732
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: July 24, 2007
Last modified: November 26, 2014
This is version 66 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3