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Q0DJ33

- GPA1_ORYSJ

UniProt

Q0DJ33 - GPA1_ORYSJ

Protein

Guanine nucleotide-binding protein alpha-1 subunit

Gene

GPA1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 2 (24 Jul 2007)
      Previous versions | rss
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    Functioni

    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi53 – 531MagnesiumBy similarity
    Metal bindingi194 – 1941MagnesiumBy similarity
    Binding sitei356 – 3561GTP; via amide nitrogenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi46 – 538GTPBy similarity
    Nucleotide bindingi188 – 1947GTPBy similarity
    Nucleotide bindingi219 – 2235GTPBy similarity
    Nucleotide bindingi288 – 2914GTPBy similarity

    GO - Molecular functioni

    1. channel regulator activity Source: RefGenome
    2. G-protein beta/gamma-subunit complex binding Source: RefGenome
    3. G-protein coupled receptor binding Source: RefGenome
    4. GTPase activity Source: RefGenome
    5. GTPase inhibitor activity Source: RefGenome
    6. GTP binding Source: UniProtKB-KW
    7. metal ion binding Source: UniProtKB-KW
    8. protein binding Source: IntAct
    9. signal transducer activity Source: RefGenome

    GO - Biological processi

    1. adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: RefGenome
    2. blue light signaling pathway Source: EnsemblPlants/Gramene
    3. cell death Source: EnsemblPlants/Gramene
    4. cellular response to abscisic acid stimulus Source: EnsemblPlants/Gramene
    5. gibberellic acid mediated signaling pathway Source: EnsemblPlants/Gramene
    6. G-protein coupled receptor signaling pathway, coupled to S1P second messenger Source: EnsemblPlants/Gramene
    7. L-phenylalanine biosynthetic process Source: EnsemblPlants/Gramene
    8. negative regulation of abscisic acid-activated signaling pathway Source: EnsemblPlants/Gramene
    9. negative regulation of catalytic activity Source: GOC
    10. positive regulation of abscisic acid-activated signaling pathway Source: EnsemblPlants/Gramene
    11. reactive oxygen species metabolic process Source: EnsemblPlants/Gramene
    12. regulation of cell proliferation Source: EnsemblPlants/Gramene
    13. response to glucose Source: EnsemblPlants/Gramene
    14. response to low fluence blue light stimulus by blue low-fluence system Source: EnsemblPlants/Gramene
    15. seed germination Source: EnsemblPlants/Gramene
    16. thylakoid membrane organization Source: EnsemblPlants/Gramene
    17. tyrosine biosynthetic process Source: EnsemblPlants/Gramene

    Keywords - Molecular functioni

    Transducer

    Keywords - Ligandi

    GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanine nucleotide-binding protein alpha-1 subunit
    Short name:
    GP-alpha-1
    Alternative name(s):
    Protein Dwarf1
    Gene namesi
    Name:GPA1
    Synonyms:D1, GA1, RGA1
    Ordered Locus Names:Os05g0333200, LOC_Os05g26890
    ORF Names:OJ1005_D04.15, OSJNBa0049D13.1
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 5

    Organism-specific databases

    GrameneiQ0DJ33.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: EnsemblPlants/Gramene
    2. heterotrimeric G-protein complex Source: RefGenome
    3. plasmodesma Source: EnsemblPlants/Gramene

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 380379Guanine nucleotide-binding protein alpha-1 subunitPRO_0000203622Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycineBy similarity
    Lipidationi5 – 51S-palmitoyl cysteineBy similarity

    Keywords - PTMi

    Lipoprotein, Myristate, Palmitate

    Interactioni

    Subunit structurei

    G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    LOC_Os03g46650Q406872EBI-1100098,EBI-1100119

    Protein-protein interaction databases

    IntActiQ0DJ33. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0DJ33.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    The helical domain (69-189) is required for self-activation.By similarity

    Sequence similaritiesi

    Belongs to the G-alpha family.Curated

    Phylogenomic databases

    eggNOGiNOG322962.
    KOiK04640.

    Family and domain databases

    Gene3Di1.10.400.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProiIPR001019. Gprotein_alpha_su.
    IPR011025. GproteinA_insert.
    IPR027417. P-loop_NTPase.
    IPR002976. Plant_Gprotein_alpha.
    [Graphical view]
    PANTHERiPTHR10218. PTHR10218. 1 hit.
    PfamiPF00503. G-alpha. 1 hit.
    [Graphical view]
    PRINTSiPR00318. GPROTEINA.
    PR01242. GPROTEINAPLT.
    SMARTiSM00275. G_alpha. 1 hit.
    [Graphical view]
    SUPFAMiSSF47895. SSF47895. 1 hit.
    SSF52540. SSF52540. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q0DJ33-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSSCSRSHS LSEAETTKNA KSADIDRRIL QETKAEQHIH KLLLLGAGES    50
    GKSTIFKQIK LLFQTGFDEA ELRSYTSVIH ANVYQTIKIL YEGAKELSQV 100
    ESDSSKYVIS PDNQEIGEKL SDIDGRLDYP LLNKELVLDV KRLWQDPAIQ 150
    ETYLRGSILQ LPDCAQYFME NLDRLAEAGY VPTKEDVLYA RVRTNGVVQI 200
    QFSPVGENKR GGEVYRLYDV GGQRNERRKW IHLFEGVNAV IFCAAISEYD 250
    QMLFEDETKN RMMETKELFD WVLKQRCFEK TSFILFLNKF DIFEKKIQKV 300
    PLSVCEWFKD YQPIAPGKQE VEHAYEFVKK KFEELYFQSS KPDRVDRVFK 350
    IYRTTALDQK LVKKTFKLID ESMRRSREGT 380
    Length:380
    Mass (Da):44,206
    Last modified:July 24, 2007 - v2
    Checksum:i4EEFAEDAB4463612
    GO

    Sequence cautioni

    The sequence BAF17140.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D38232 mRNA. Translation: BAA07405.1.
    AC117264 Genomic DNA. Translation: AAV43839.1.
    AC144739 Genomic DNA. Translation: AAW57778.2.
    AP008211 Genomic DNA. Translation: BAF17140.1. Different initiation.
    RefSeqiNP_001055226.1. NM_001061761.1.
    UniGeneiOs.5302.

    Genome annotation databases

    GeneIDi4338448.
    KEGGiosa:4338448.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D38232 mRNA. Translation: BAA07405.1 .
    AC117264 Genomic DNA. Translation: AAV43839.1 .
    AC144739 Genomic DNA. Translation: AAW57778.2 .
    AP008211 Genomic DNA. Translation: BAF17140.1 . Different initiation.
    RefSeqi NP_001055226.1. NM_001061761.1.
    UniGenei Os.5302.

    3D structure databases

    ProteinModelPortali Q0DJ33.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q0DJ33. 1 interaction.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 4338448.
    KEGGi osa:4338448.

    Organism-specific databases

    Gramenei Q0DJ33.

    Phylogenomic databases

    eggNOGi NOG322962.
    KOi K04640.

    Family and domain databases

    Gene3Di 1.10.400.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProi IPR001019. Gprotein_alpha_su.
    IPR011025. GproteinA_insert.
    IPR027417. P-loop_NTPase.
    IPR002976. Plant_Gprotein_alpha.
    [Graphical view ]
    PANTHERi PTHR10218. PTHR10218. 1 hit.
    Pfami PF00503. G-alpha. 1 hit.
    [Graphical view ]
    PRINTSi PR00318. GPROTEINA.
    PR01242. GPROTEINAPLT.
    SMARTi SM00275. G_alpha. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47895. SSF47895. 1 hit.
    SSF52540. SSF52540. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of a cDNA for the alpha subunit of a G protein from rice."
      Ishikawa A., Tsubouchi H., Iwasaki Y., Asahi T.
      Plant Cell Physiol. 36:353-359(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Nipponbare.
      Tissue: Leaf.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    4. "The rice annotation project database (RAP-DB): 2008 update."
      The rice annotation project (RAP)
      Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: cv. Nipponbare.

    Entry informationi

    Entry nameiGPA1_ORYSJ
    AccessioniPrimary (citable) accession number: Q0DJ33
    Secondary accession number(s): P49083
    , Q2HNY6, Q43604, Q5KQF9, Q5W732
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 24, 2007
    Last sequence update: July 24, 2007
    Last modified: October 1, 2014
    This is version 64 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3