Q0DJ33 (GPA1_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Guanine nucleotide-binding protein alpha-1 subunit
Short name=GP-alpha-1

Alternative name(s):
Protein Dwarf1

Gene names

Name:	GPA1
Synonyms:	D1, GA1, RGA1
Ordered Locus Names:	Os05g0333200, LOC_Os05g26890
ORF Names:	OJ1005_D04.15, OSJNBa0049D13.1

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	380 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.
Subunit structure	G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.
Domain	The helical domain (69-189) is required for self-activation By similarity.
Sequence similarities	Belongs to the G-alpha family.
Sequence caution	The sequence BAF17140.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Transducer
PTM	Lipoprotein Myristate Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G-protein coupled receptor signaling pathway, coupled to S1P second messenger Inferred from electronic annotation. Source: EnsemblPlants/Gramene L-phenylalanine biosynthetic process Inferred from electronic annotation. Source: EnsemblPlants/Gramene adenylate cyclase-modulating G-protein coupled receptor signaling pathway Inferred from sequence or structural similarity. Source: RefGenome cell death Inferred from electronic annotation. Source: EnsemblPlants/Gramene gibberellic acid mediated signaling pathway Inferred from electronic annotation. Source: EnsemblPlants/Gramene positive regulation of abscisic acid mediated signaling pathway Inferred from electronic annotation. Source: EnsemblPlants/Gramene regulation of cell proliferation Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to glucose stimulus Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to low fluence blue light stimulus by blue low-fluence system Inferred from electronic annotation. Source: EnsemblPlants/Gramene seed germination Inferred from electronic annotation. Source: EnsemblPlants/Gramene thylakoid membrane organization Inferred from electronic annotation. Source: EnsemblPlants/Gramene tyrosine biosynthetic process Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: EnsemblPlants/Gramene heterotrimeric G-protein complex Inferred from sequence or structural similarity. Source: RefGenome
Molecular_function	G-protein beta/gamma-subunit complex binding Inferred from sequence or structural similarity. Source: RefGenome G-protein coupled receptor binding Inferred from sequence or structural similarity. Source: RefGenome GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from sequence or structural similarity. Source: RefGenome GTPase inhibitor activity Inferred from sequence or structural similarity. Source: RefGenome channel regulator activity Inferred from sequence or structural similarity. Source: RefGenome metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW signal transducer activity Inferred from sequence or structural similarity. Source: RefGenome
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
LOC_Os03g46650	Q40687	2	EBI-1100098,EBI-1100119

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 380	379	Guanine nucleotide-binding protein alpha-1 subunit		PRO_0000203622
Regions
	Nucleotide binding	46 – 53	8	GTP By similarity
	Nucleotide binding	188 – 194	7	GTP By similarity
	Nucleotide binding	219 – 223	5	GTP By similarity
	Nucleotide binding	288 – 291	4	GTP By similarity
Sites
	Metal binding	53	1	Magnesium By similarity
	Metal binding	194	1	Magnesium By similarity
	Binding site	356	1	GTP; via amide nitrogen By similarity
Amino acid modifications
	Lipidation	2	1	N-myristoyl glycine By similarity
	Lipidation	5	1	S-palmitoyl cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0DJ33 [UniParc]. Last modified July 24, 2007. Version 2. Checksum: 4EEFAEDAB4463612	FASTA	380	44,206
	10 20 30 40 50 60 MGSSCSRSHS LSEAETTKNA KSADIDRRIL QETKAEQHIH KLLLLGAGES GKSTIFKQIK 70 80 90 100 110 120 LLFQTGFDEA ELRSYTSVIH ANVYQTIKIL YEGAKELSQV ESDSSKYVIS PDNQEIGEKL 130 140 150 160 170 180 SDIDGRLDYP LLNKELVLDV KRLWQDPAIQ ETYLRGSILQ LPDCAQYFME NLDRLAEAGY 190 200 210 220 230 240 VPTKEDVLYA RVRTNGVVQI QFSPVGENKR GGEVYRLYDV GGQRNERRKW IHLFEGVNAV 250 260 270 280 290 300 IFCAAISEYD QMLFEDETKN RMMETKELFD WVLKQRCFEK TSFILFLNKF DIFEKKIQKV 310 320 330 340 350 360 PLSVCEWFKD YQPIAPGKQE VEHAYEFVKK KFEELYFQSS KPDRVDRVFK IYRTTALDQK 370 380 LVKKTFKLID ESMRRSREGT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of a cDNA for the alpha subunit of a G protein from rice." Ishikawa A., Tsubouchi H., Iwasaki Y., Asahi T. Plant Cell Physiol. 36:353-359(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Nipponbare. Tissue: Leaf.
[2]	"A fine physical map of the rice chromosome 5." Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J., Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F., Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y. Mol. Genet. Genomics 274:337-345(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D38232 mRNA. Translation: BAA07405.1. AC117264 Genomic DNA. Translation: AAV43839.1. AC144739 Genomic DNA. Translation: AAW57778.2. AP008211 Genomic DNA. Translation: BAF17140.1. Different initiation.
RefSeq	NP_001055226.1. NM_001061761.1.
UniGene	Os.5302.
3D structure databases
ProteinModelPortal	Q0DJ33.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q0DJ33. 1 interaction.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4338448.
KEGG	osa:4338448.
Organism-specific databases
Gramene	Q0DJ33.
Phylogenomic databases
eggNOG	NOG322962.
KO	K04640.
ProtClustDB	CLSN2683295.
Gene expression databases
ArrayExpress	Q0DJ33.
Family and domain databases
Gene3D	1.10.400.10. 1 hit.
InterPro	IPR001019. Gprotein_alpha_su. IPR011025. GproteinA_insert. IPR002976. Plant_Gprotein_alpha. [Graphical view]
PANTHER	PTHR10218. PTHR10218. 1 hit. PTHR10218:SF25. PTHR10218:SF25. 1 hit.
Pfam	PF00503. G-alpha. 1 hit. [Graphical view]
PRINTS	PR00318. GPROTEINA. PR01242. GPROTEINAPLT.
SMART	SM00275. G_alpha. 1 hit. [Graphical view]
SUPFAM	SSF47895. Transducn_insert. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GPA1_ORYSJ

Accession

Primary (citable) accession number: Q0DJ33
Secondary accession number(s): P49083 , Q2HNY6, Q43604, Q5KQF9, Q5W732

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	July 24, 2007
Last modified:	May 1, 2013
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program