Reviewed, UniProtKB/Swiss-Prot Q0DIV0 (HEMH2_ORYSJ)

Names and origin

Protein names

Recommended name:
    Ferrochelatase-2, chloroplastic
    EC=4.99.1.1
Alternative name(s):
    Ferrochelatase II
    Protoheme ferro-lyase 2
    Heme synthetase 2

Gene names

Ordered Locus Names:	Os05g0361200, LOC_Os05g29760
ORF Names:	P0530H10.9, P0692D12.2

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	526 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX.
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+.
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1.
Subcellular location	Plastid › chloroplast By similarity.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Iron
Molecular function	Lyase
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferrochelatase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast Potential
	Chain	? – 526		Ferrochelatase-2, chloroplastic		PRO_0000008884

Sequences

	Sequence		Length	Mass (Da)
	Q0DIV0-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 95137FEEDA8F3E5F	FASTA	526	58,590
	10 20 30 40 50 60 MWSSSQASTR GVIEVGRVEA GPSHFPKRPA PRNSSRVNLS RTYAIKSCSV SSRTGLCLGQ 70 80 90 100 110 120 CYHKKSSACK CKLGWSSQPL SSLRHHLRVH SSASEAVLTS QSDFTKLLVG NEKIGVLLLN 130 140 150 160 170 180 LGGPETLDDV QPFLFNLFAD PDIIRLPRLF RFLQKPLAQF ISVVRAPKSK EGYASIGGGS 190 200 210 220 230 240 PLRQITDAQA EALRKALCDK DIPAKVYVGM RYWHPFTEEA IEQIKRDGIT KLVVLPLYPQ 250 260 270 280 290 300 FSISTSGSSL RLLEGIFRED EYLVNMQHTV IPSWYQREGY IKAMATLIEK ELRTFSEPQK 310 320 330 340 350 360 VMIFFSAHGV PLAYVEEAGD PYKAEMEECV DLIMEELEKR GITNSCTLAY QSRVGPVEWL 370 380 390 400 410 420 RPYTDETIIE LGQKGVKSLL AVPISFVSEH IETLEEIDVE YKELALESGI KHWGRVPALG 430 440 450 460 470 480 CEPTFITDLA DAVIESLPYV GAMAVSNLEA RQPLVPLGSV EELLAAYDSK RDELPPPVTV 490 500 510 520 WEWGWTKSAE TWNGRAAMLA VLALLVLEVT TGEGFLHQWG ILPLFH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A fine physical map of the rice chromosome 5." Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J., Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F., Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y. Mol. Genet. Genomics 274:337-345(2005) [PubMed: 16261349] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC134348 Genomic DNA. Translation: AAT38095.1. AC135929 Genomic DNA. Translation: AAV31391.1. AP008211 Genomic DNA. Translation: BAF17223.1. AK073873 mRNA. Translation: BAG93685.1.
RefSeq	NP_001055309.1.
UniGene	Os.8611
3D structure databases
HSSP	HSSP built from PDB template 1HRK based on UniProtKB P22830.
ModBase	Search...
Genome annotation databases
GeneID	4338537.
KEGG	osa:4338537.
Organism-specific databases
Gramene	O22101.
Family and domain databases
InterPro	IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
ProDom	PD002792. Ferrochelatase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00109. hemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH2_ORYSJ

Accession

Primary (citable) accession number: Q0DIV0
Secondary accession number(s): B7EMT8, O22101, Q6L4M5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	October 17, 2006
Last modified:	June 16, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)