Reviewed, UniProtKB/Swiss-Prot Q0DHL2 (LAC12_ORYSJ)

Names and origin

Protein names

Recommended name:
    Laccase-12/13
    EC=1.10.3.2
Alternative name(s):
    Benzenediol:oxygen oxidoreductase 12/13
    Urishiol oxidase 12/13
    Diphenol oxidase 12/13

Gene names

Name:	LAC12
Ordered Locus Names:	Os05g0458500, LOC_Os05g38410
ORF Names:	OJ1362_D02.9, OsJ_018046
AND
Name:	LAC13
Ordered Locus Names:	Os05g0458600, LOC_Os05g38420
ORF Names:	OJ1362_D02.10

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	574 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Lignin degradation and detoxification of lignin-derived products By similarity.
Catalytic activity	4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O.
Cofactor	Binds 4 copper ions per monomer By similarity.
Subcellular location	Secreted › extracellular space › apoplastPotential.
Sequence similarities	Belongs to the multicopper oxidase family. Contains 3 plastocyanin-like domains.
Sequence caution	The sequence AAU44018.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAU44019.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AK068047 differs from that shown. Reason: Frameshift at position 77. The sequence BAF17660.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence EAZ34563.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Lignin degradation
Cellular component	Apoplast Secreted
Domain	Repeat Signal
Ligand	Copper Metal-binding
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	lignin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	copper ion binding Inferred from electronic annotation. Source: InterPro laccase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 574	547	Laccase-12/13		PRO_0000291897
Regions
	Domain	35 – 151	117	Plastocyanin-like 1
	Domain	161 – 314	154	Plastocyanin-like 2
	Domain	424 – 558	135	Plastocyanin-like 3
Sites
	Metal binding	85	1	Copper 1 By similarity
	Metal binding	87	1	Copper 2 By similarity
	Metal binding	130	1	Copper 2 By similarity
	Metal binding	132	1	Copper 3 By similarity
	Metal binding	475	1	Copper 4 By similarity
	Metal binding	478	1	Copper 1 By similarity
	Metal binding	480	1	Copper 3 By similarity
	Metal binding	537	1	Copper 3 By similarity
	Metal binding	538	1	Copper 4 By similarity
	Metal binding	539	1	Copper 2 By similarity
	Metal binding	543	1	Copper 4 By similarity
Amino acid modifications
	Glycosylation	81	1	N-linked (GlcNAc...) Potential
	Glycosylation	173	1	N-linked (GlcNAc...) Potential
	Glycosylation	190	1	N-linked (GlcNAc...) Potential
	Glycosylation	206	1	N-linked (GlcNAc...) Potential
	Glycosylation	242	1	N-linked (GlcNAc...) Potential
	Glycosylation	302	1	N-linked (GlcNAc...) Potential
	Glycosylation	335	1	N-linked (GlcNAc...) Potential
	Glycosylation	342	1	N-linked (GlcNAc...) Potential
	Glycosylation	381	1	N-linked (GlcNAc...) Potential
	Glycosylation	388	1	N-linked (GlcNAc...) Potential
	Glycosylation	398	1	N-linked (GlcNAc...) Potential
	Glycosylation	434	1	N-linked (GlcNAc...) Potential
	Glycosylation	441	1	N-linked (GlcNAc...) Potential
	Glycosylation	447	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q0DHL2-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 27DD915DD4F41C13	FASTA	574	62,348
	10 20 30 40 50 60 MAAASSVLRC CLLVAALMTL SAMGAEAITR QYLFDVQTTS VTRLCSTKSI VTVNGQYPGP 70 80 90 100 110 120 TLFAREGDHV EVTVVNHSPY NMSIHWHGIR QLLSGWADGP SYITQCPIQP GGSYVYRFTI 130 140 150 160 170 180 TGQRGTLWWH AHISWLRATV HGPMVILPPA GVGYPFPAPH EEVPIMFGEW WNNDTEAVIS 190 200 210 220 230 240 QALQTGGGPN ISDAYTLNGL PGPLYNCSAQ DTFKLKVKPG KTYMLRLINA ALNDELFFSI 250 260 270 280 290 300 ANHTLTVVDV DALYVKPFTV DTLIIAPGQT SNVLLTAKPT YPGASYYMLA RPYTTTQGTF 310 320 330 340 350 360 DNTTVAGVLE YDDPCPTTAA GKIVPIFSPT LPQINDTNAV SNFTAKLRSL ASAGYPAAVP 370 380 390 400 410 420 QQVDHRFFFT VGLGTHPCAV NGTCQGPNGS RFAASINNVS FVLPATALLQ SHFAGKSKGV 430 440 450 460 470 480 YASNFPYYPL NPFNYTGTPP NNTNVMNGTK VLVLPYGANV ELVMQDTSIL GAESHPLHLH 490 500 510 520 530 540 GFNFFVVGQG FGNFDPINDP AKFNLYDPVE RNTVGVPAGG WVAIRFHADN PGVWFMHCHL 550 560 570 EVHMSWGLKM AWLVLDGSRP DQKLPPPPLD LPKC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A fine physical map of the rice chromosome 5." Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J., Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F., Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y. Mol. Genet. Genomics 274:337-345(2005) [PubMed: 16261349] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (OS05G0458500 AND OS05G0458600). Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (OS05G0458500 AND OS05G0458600). Strain: cv. Nipponbare.
[3]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (OS05G0458500 AND OS05G0458600). Strain: cv. Nipponbare.
[5]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (OS05G0458600). Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC105770 Genomic DNA. Translation: AAU44018.1. Sequence problems. AP008211 Genomic DNA. Translation: BAF17660.1. Sequence problems. CM000142 Genomic DNA. Translation: EAZ34563.1. Sequence problems. AP008211 Genomic DNA. Translation: BAF17661.1. AC105770 Genomic DNA. Translation: AAU44019.1. Sequence problems. AK068047 mRNA. No translation available.
RefSeq	NP_001055746.1. NP_001055747.1.
UniGene	Os.51049
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4339003. 4339004.
KEGG	osa:4339003. osa:4339004.
Family and domain databases
InterPro	IPR001117. Cu-oxidase. IPR011706. Cu-oxidase_2. IPR011707. Cu-oxidase_3. IPR002355. Cu_oxidase_Cu_BS. IPR008972. Cupredoxin. IPR017761. Laccase. [Graphical view]
Gene3D	G3DSA:2.60.40.420. Cupredoxin. 3 hits.
Pfam	PF00394. Cu-oxidase. 1 hit. PF07731. Cu-oxidase_2. 1 hit. PF07732. Cu-oxidase_3. 1 hit. [Graphical view]
PROSITE	PS00079. MULTICOPPER_OXIDASE1. 1 hit. PS00080. MULTICOPPER_OXIDASE2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LAC12_ORYSJ

Accession

Primary (citable) accession number: Q0DHL2
Secondary accession number(s): Q0DHL3, Q65XF7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	October 17, 2006
Last modified:	November 25, 2008
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)