Q0DHB7 (EXPA4_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Expansin-A4

Alternative name(s):
Alpha-expansin-4
OsEXP4
OsEXPA4
OsaEXPa1.22

Gene names

Name:	EXPA4
Synonyms:	EXP4
Ordered Locus Names:	Os05g0477600, LOC_Os05g39990
ORF Names:	OSJNBa0018K15.5, OsJ_018161

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Causes loosening and extension of plant cell walls by disrupting non-covalent bonding between cellulose microfibrils and matrix glucans. No enzymatic activity has been found. Required for normal plant growth. May be required for rapid internodal elongation in deepwater rice during submergence. Ref.7
Subcellular location	Secreted › cell wall By similarity. Membrane; Peripheral membrane protein By similarity.
Tissue specificity	Expressed in lateral root primordia, adventitious root primordia, coleoptiles, shoot apex, leaf primordia, very young leaves, panicles and flowers. Ref.6 Ref.9
Induction	By gibberellin (GA3), submergence and wounding. Ref.6
Disruption phenotype	Plants are smaller. Ref.7
Sequence similarities	Belongs to the expansin family. Expansin A subfamily. Contains 1 expansin-like CBD domain. Contains 1 expansin-like EG45 domain.

Ontologies

Keywords
Cellular component	Cell wall Membrane Secreted
Domain	Signal
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	plant-type cell wall organization Inferred from electronic annotation. Source: InterPro
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 246	228	Expansin-A4		PRO_0000251983
Regions
	Domain	42 – 154	113	Expansin-like EG45
	Domain	164 – 243	80	Expansin-like CBD
Amino acid modifications
	Disulfide bond	45 ↔ 73		By similarity
	Disulfide bond	76 ↔ 149		By similarity
	Disulfide bond	81 ↔ 88		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0DHB7 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: DFAA1224D8A3F9A0	FASTA	246	25,884
	10 20 30 40 50 60 MAIAGVLFLL FLARQASAAG YGGWQSAHAT FYGGGDASGT MGGACGYGNL YSQGYGTNTA 70 80 90 100 110 120 ALSTALFNDG AACGSCYELR CDNAGSSCLP GSITVTATNF CPPNYGLPSD DGGWCNPPRP 130 140 150 160 170 180 HFDMAEPAFL HIAQYRAGIV PVSFRRVPCV KKGGVRFTVN GHSYFNLVLV TNVAGAGDVR 190 200 210 220 230 240 SVSIKGSRTG WQPMSRNWGQ NWQSNAFLDG QSLSFQVTAS DGRTVTSNNV AHPGWQFGQT FEGGQF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of alpha-expansin and expansin-like genes in deepwater rice." Lee Y., Kende H. Plant Physiol. 130:1396-1405(2002) [PubMed: 12428004] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"A fine physical map of the rice chromosome 5." Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J., Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F., Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y. Mol. Genet. Genomics 274:337-345(2005) [PubMed: 16261349] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed: 18089549] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[5]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[6]	"Expression of alpha-expansin genes in young seedlings of rice (Oryza sativa L.)." Huang J., Takano T., Akita S. Planta 211:467-473(2000) [PubMed: 11030545] [Abstract] Cited for: TISSUE SPECIFICITY, INDUCTION.
[7]	"Regulation of expansin gene expression affects growth and development in transgenic rice plants." Choi D., Lee Y., Cho H.-T., Kende H. Plant Cell 15:1386-1398(2003) [PubMed: 12782731] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[8]	"Nomenclature for members of the expansin superfamily of genes and proteins." Kende H., Bradford K.J., Brummell D.A., Cho H.-T., Cosgrove D.J., Fleming A.J., Gehring C., Lee Y., McQueen-Mason S.J., Rose J.K.C., Voesenek L.A.C. Plant Mol. Biol. 55:311-314(2004) [PubMed: 15604683] [Abstract] Cited for: NOMENCLATURE.
[9]	"Characterization and transcriptional expression of the alpha-expansin gene family in rice." Shin J.-H., Jeong D.-H., Park M.C., An G. Mol. Cells 20:210-218(2005) [PubMed: 16267395] [Abstract] Cited for: TISSUE SPECIFICITY.

Web resources

EXPANSIN homepage

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF394545 Genomic DNA. Translation: AAL24481.1. AC144737 Genomic DNA. Translation: AAT01362.1. AP008211 Genomic DNA. Translation: BAF17756.1. CM000142 Genomic DNA. Translation: EAZ34678.1.
RefSeq	NP_001055842.1. NM_001062377.1.
UniGene	Os.50825.
3D structure databases
ProteinModelPortal	Q0DHB7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os05g39990.1; LOC_Os05g39990.1; LOC_Os05g39990.
GeneID	4339106.
KEGG	osa:4339106.
Organism-specific databases
Gramene	P93442.
Phylogenomic databases
GeneTree	EPGT00070000027927.
HOGENOM	HBG605129.
OMA	PNFALAN.
PhylomeDB	Q0DHB7.
ProtClustDB	CLSN2691979.
Family and domain databases
InterPro	IPR014733. Barwin-like_endoglucanase. IPR009009. Barwin-related_endoglucanase. IPR007118. Expan_Lol_pI. IPR007117. Expan_Lol_pI_C. IPR002963. Expansin. IPR007112. Expansin/allergen_DPBB_dom. [Graphical view]
Gene3D	G3DSA:2.40.40.10. Barwin. 1 hit. G3DSA:2.60.40.760. Expan_Lol_pI_C. 1 hit.
Pfam	PF03330. DPBB_1. 1 hit. PF01357. Pollen_allerg_1. 1 hit. [Graphical view]
PRINTS	PR01226. EXPANSIN. PR01225. EXPANSNFAMLY.
SMART	SM00837. DPBB_1. 1 hit. [Graphical view]
SUPFAM	SSF50685. Barwin_like. 1 hit. SSF49590. Expan_Lol_pI_C. 1 hit.
PROSITE	PS50843. EXPANSIN_CBD. 1 hit. PS50842. EXPANSIN_EG45. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EXPA4_ORYSJ

Accession

Primary (citable) accession number: Q0DHB7
Secondary accession number(s): P93442, Q75GN4, Q946J0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	October 17, 2006
Last modified:	December 14, 2011
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program