Reviewed, UniProtKB/Swiss-Prot Q0DGD7 (BGAL8_ORYSJ)

Names and origin

Protein names

Recommended name:
    Beta-galactosidase 8
      Short name=Lactase 8
    EC=3.2.1.23

Gene names

Ordered Locus Names:	Os05g0539400, LOC_Os05g46200
ORF Names:	OSJNBa0052K01.15

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	673 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Subcellular location	Secreted › extracellular space › apoplast Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 35 family.
Sequence caution	The sequence AAV59405.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Apoplast Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 673	653	Beta-galactosidase 8		PRO_0000294160
Sites
	Active site	189	1	Proton donor Potential
	Active site	272	1	Nucleophile Potential
Amino acid modifications
	Glycosylation	38	1	N-linked (GlcNAc...) Potential
	Glycosylation	230	1	N-linked (GlcNAc...) Potential
	Glycosylation	304	1	N-linked (GlcNAc...) Potential
	Glycosylation	329	1	N-linked (GlcNAc...) Potential
	Glycosylation	401	1	N-linked (GlcNAc...) Potential
	Glycosylation	489	1	N-linked (GlcNAc...) Potential
	Glycosylation	540	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q0DGD7-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 6C323CDBBAB33AB6	FASTA	673	75,674
	10 20 30 40 50 60 MGPSRSFQNL LLLLLPLALA LCSAAASGEA SRRFWVENDT FWKDGAPFQI VGGDVHYFRI 70 80 90 100 110 120 VPEYWKDRLL RAKALGLNTI QTYVPWNLHE PKPLSWEFKG FTDIESYLRL AHELDMLVML 130 140 150 160 170 180 RVGPYICGEW DLGGFPPWLL TIEPTIELRS SDSTYLSLVD RWWGVLLPKI APLLYSNGGP 190 200 210 220 230 240 IIMVQIENEF GSFGDDKNYL HYLVEVARRY LGNDIMLYTT DGGAIGNLKN GTILQDDVFA 250 260 270 280 290 300 AVDFDTGSNP WPIFQLQKEY NLPGKSAPLS SEFYTGWLTH WGERIATTDA SSTAKALKRI 310 320 330 340 350 360 LCRNGSAVLY MAHGGTNFGF YNGANTGQNE SDYKADLTSY DYDAPIREYG DVHNAKYKAL 370 380 390 400 410 420 RRVIHECTGI PLLQLPSKIE RASYGLVEVQ KVASLFDVIH NISDALKVAF SEQPLSMELM 430 440 450 460 470 480 GQMFGFLLYT SEYQEKHSSS ILSIPKVHDR AQVFVSCSHG DVRKPRYVGI VERWSSKTLQ 490 500 510 520 530 540 IPSLSCSSNV SLYILVENMG RVNYGPYIFD QKGILSSVEI DGIILRHWKM HPVSLNAVGN 550 560 570 580 590 600 LSKLQLIMQM TDAEASKVSI YGDSENKLQD VSLYLNEGIS EEPAFYEGHF HIDSESEKKD 610 620 630 640 650 660 TFISFRGWNK GVAFVNNFNI GRFWPAIGPQ CALYVPAPIL KPGDNVIVIF ELHSPNPELT 670 IKLVKDPDFT CGQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A fine physical map of the rice chromosome 5." Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J., Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F., Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y. Mol. Genet. Genomics 274:337-345(2005) [PubMed: 16261349] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC119291 Genomic DNA. Translation: AAV59405.1. Sequence problems. AP008211 Genomic DNA. Translation: BAF18086.1. AK068572 mRNA. No translation available.
RefSeq	NP_001056172.1.
UniGene	Os.14570
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH35. Glycoside Hydrolase Family 35.
Genome annotation databases
GeneID	4339457.
KEGG	osa:4339457.
Organism-specific databases
Gramene	Q53WK7.
Phylogenomic databases
HOGENOM	HBG292753.
Family and domain databases
InterPro	IPR019801. Glyco_hydro_35_CS. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. IPR001944. Glycoside_Hdrlase_35. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR23421. Glyco_hydro_35. 1 hit.
Pfam	PF01301. Glyco_hydro_35. 1 hit. [Graphical view]
PRINTS	PR00742. GLHYDRLASE35.
PROSITE	PS01182. GLYCOSYL_HYDROL_F35. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL8_ORYSJ

Accession

Primary (citable) accession number: Q0DGD7
Secondary accession number(s): Q53WK7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	October 17, 2006
Last modified:	December 15, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)