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Protein

Beta-galactosidase 8

Gene

Os05g0539400

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei189 – 1891Proton donorSequence Analysis
Active sitei272 – 2721NucleophileSequence Analysis

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. glucuronoxylan metabolic process Source: EnsemblPlants/Gramene
  2. hydrogen peroxide biosynthetic process Source: EnsemblPlants/Gramene
  3. protein desumoylation Source: EnsemblPlants/Gramene
  4. vegetative to reproductive phase transition of meristem Source: EnsemblPlants/Gramene
  5. xylan biosynthetic process Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

ReactomeiREACT_273539. Glycosphingolipid metabolism.
REACT_296633. Keratan sulfate degradation.
REACT_351566. HS-GAG degradation.

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase 8 (EC:3.2.1.23)
Short name:
Lactase 8
Gene namesi
Ordered Locus Names:Os05g0539400, LOC_Os05g46200
ORF Names:OSJNBa0052K01.15
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763 Componenti: Chromosome 5

Organism-specific databases

GrameneiQ0DGD7.

Subcellular locationi

GO - Cellular componenti

  1. apoplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 673653Beta-galactosidase 8PRO_0000294160Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi38 – 381N-linked (GlcNAc...)Sequence Analysis
Glycosylationi230 – 2301N-linked (GlcNAc...)Sequence Analysis
Glycosylationi304 – 3041N-linked (GlcNAc...)Sequence Analysis
Glycosylationi329 – 3291N-linked (GlcNAc...)Sequence Analysis
Glycosylationi401 – 4011N-linked (GlcNAc...)Sequence Analysis
Glycosylationi489 – 4891N-linked (GlcNAc...)Sequence Analysis
Glycosylationi540 – 5401N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Expressioni

Gene expression databases

ExpressionAtlasiQ0DGD7. baseline.

Structurei

3D structure databases

ProteinModelPortaliQ0DGD7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1874.
HOGENOMiHOG000221607.
InParanoidiQ0DGD7.
KOiK12309.
OMAiHRIQMAK.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR026283. B-gal_1-like.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF13364. BetaGal_dom4_5. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PIRSFiPIRSF006336. B-gal. 1 hit.
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0DGD7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGPSRSFQNL LLLLLPLALA LCSAAASGEA SRRFWVENDT FWKDGAPFQI
60 70 80 90 100
VGGDVHYFRI VPEYWKDRLL RAKALGLNTI QTYVPWNLHE PKPLSWEFKG
110 120 130 140 150
FTDIESYLRL AHELDMLVML RVGPYICGEW DLGGFPPWLL TIEPTIELRS
160 170 180 190 200
SDSTYLSLVD RWWGVLLPKI APLLYSNGGP IIMVQIENEF GSFGDDKNYL
210 220 230 240 250
HYLVEVARRY LGNDIMLYTT DGGAIGNLKN GTILQDDVFA AVDFDTGSNP
260 270 280 290 300
WPIFQLQKEY NLPGKSAPLS SEFYTGWLTH WGERIATTDA SSTAKALKRI
310 320 330 340 350
LCRNGSAVLY MAHGGTNFGF YNGANTGQNE SDYKADLTSY DYDAPIREYG
360 370 380 390 400
DVHNAKYKAL RRVIHECTGI PLLQLPSKIE RASYGLVEVQ KVASLFDVIH
410 420 430 440 450
NISDALKVAF SEQPLSMELM GQMFGFLLYT SEYQEKHSSS ILSIPKVHDR
460 470 480 490 500
AQVFVSCSHG DVRKPRYVGI VERWSSKTLQ IPSLSCSSNV SLYILVENMG
510 520 530 540 550
RVNYGPYIFD QKGILSSVEI DGIILRHWKM HPVSLNAVGN LSKLQLIMQM
560 570 580 590 600
TDAEASKVSI YGDSENKLQD VSLYLNEGIS EEPAFYEGHF HIDSESEKKD
610 620 630 640 650
TFISFRGWNK GVAFVNNFNI GRFWPAIGPQ CALYVPAPIL KPGDNVIVIF
660 670
ELHSPNPELT IKLVKDPDFT CGQ
Length:673
Mass (Da):75,674
Last modified:October 17, 2006 - v1
Checksum:i6C323CDBBAB33AB6
GO

Sequence cautioni

The sequence AAV59405.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC119291 Genomic DNA. Translation: AAV59405.1. Sequence problems.
AP008211 Genomic DNA. Translation: BAF18086.1.
AK068572 mRNA. No translation available.
RefSeqiNP_001056172.1. NM_001062707.1.
UniGeneiOs.14570.

Genome annotation databases

EnsemblPlantsiOS05T0539400-01; OS05T0539400-01; OS05G0539400.
GeneIDi4339457.
KEGGiosa:4339457.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC119291 Genomic DNA. Translation: AAV59405.1. Sequence problems.
AP008211 Genomic DNA. Translation: BAF18086.1.
AK068572 mRNA. No translation available.
RefSeqiNP_001056172.1. NM_001062707.1.
UniGeneiOs.14570.

3D structure databases

ProteinModelPortaliQ0DGD7.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS05T0539400-01; OS05T0539400-01; OS05G0539400.
GeneIDi4339457.
KEGGiosa:4339457.

Organism-specific databases

GrameneiQ0DGD7.

Phylogenomic databases

eggNOGiCOG1874.
HOGENOMiHOG000221607.
InParanoidiQ0DGD7.
KOiK12309.
OMAiHRIQMAK.

Enzyme and pathway databases

ReactomeiREACT_273539. Glycosphingolipid metabolism.
REACT_296633. Keratan sulfate degradation.
REACT_351566. HS-GAG degradation.

Gene expression databases

ExpressionAtlasiQ0DGD7. baseline.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR026283. B-gal_1-like.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF13364. BetaGal_dom4_5. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PIRSFiPIRSF006336. B-gal. 1 hit.
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  4. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Nipponbare.

Entry informationi

Entry nameiBGAL8_ORYSJ
AccessioniPrimary (citable) accession number: Q0DGD7
Secondary accession number(s): Q53WK7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: October 17, 2006
Last modified: April 1, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.