Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Beta-galactosidase 8

Gene

Os05g0539400

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei189Proton donorSequence analysis1
Active sitei272NucleophileSequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase

Enzyme and pathway databases

ReactomeiR-OSA-1660662. Glycosphingolipid metabolism.
R-OSA-2022857. Keratan sulfate degradation.
R-OSA-2024096. HS-GAG degradation.
R-OSA-6798695. Neutrophil degranulation.

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase 8 (EC:3.2.1.23)
Short name:
Lactase 8
Gene namesi
Ordered Locus Names:Os05g0539400, LOC_Os05g46200
ORF Names:OsJ_19375Imported, OSJNBa0052K01.15
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Apoplast, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000029416021 – 673Beta-galactosidase 8Add BLAST653

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi38N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi230N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi304N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi329N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi401N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi489N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi540N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ0DGD7.

Expressioni

Gene expression databases

GenevisibleiQ0DGD7. OS.

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os05g46200.1.

Structurei

3D structure databases

ProteinModelPortaliQ0DGD7.
SMRiQ0DGD7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0496. Eukaryota.
COG1874. LUCA.
HOGENOMiHOG000221607.
InParanoidiQ0DGD7.
KOiK12309.
OMAiGWGKGIV.
OrthoDBiEOG093603DJ.

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
InterProiView protein in InterPro
IPR026283. B-gal_1-like.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like_sf.
IPR031330. Gly_Hdrlase_35_cat.
IPR019801. Glyco_hydro_35_CS.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiView protein in Pfam
PF13364. BetaGal_dom4_5. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
PIRSFiPIRSF006336. B-gal. 1 hit.
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiView protein in PROSITE
PS01182. GLYCOSYL_HYDROL_F35. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0DGD7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGPSRSFQNL LLLLLPLALA LCSAAASGEA SRRFWVENDT FWKDGAPFQI
60 70 80 90 100
VGGDVHYFRI VPEYWKDRLL RAKALGLNTI QTYVPWNLHE PKPLSWEFKG
110 120 130 140 150
FTDIESYLRL AHELDMLVML RVGPYICGEW DLGGFPPWLL TIEPTIELRS
160 170 180 190 200
SDSTYLSLVD RWWGVLLPKI APLLYSNGGP IIMVQIENEF GSFGDDKNYL
210 220 230 240 250
HYLVEVARRY LGNDIMLYTT DGGAIGNLKN GTILQDDVFA AVDFDTGSNP
260 270 280 290 300
WPIFQLQKEY NLPGKSAPLS SEFYTGWLTH WGERIATTDA SSTAKALKRI
310 320 330 340 350
LCRNGSAVLY MAHGGTNFGF YNGANTGQNE SDYKADLTSY DYDAPIREYG
360 370 380 390 400
DVHNAKYKAL RRVIHECTGI PLLQLPSKIE RASYGLVEVQ KVASLFDVIH
410 420 430 440 450
NISDALKVAF SEQPLSMELM GQMFGFLLYT SEYQEKHSSS ILSIPKVHDR
460 470 480 490 500
AQVFVSCSHG DVRKPRYVGI VERWSSKTLQ IPSLSCSSNV SLYILVENMG
510 520 530 540 550
RVNYGPYIFD QKGILSSVEI DGIILRHWKM HPVSLNAVGN LSKLQLIMQM
560 570 580 590 600
TDAEASKVSI YGDSENKLQD VSLYLNEGIS EEPAFYEGHF HIDSESEKKD
610 620 630 640 650
TFISFRGWNK GVAFVNNFNI GRFWPAIGPQ CALYVPAPIL KPGDNVIVIF
660 670
ELHSPNPELT IKLVKDPDFT CGQ
Length:673
Mass (Da):75,674
Last modified:October 17, 2006 - v1
Checksum:i6C323CDBBAB33AB6
GO

Sequence cautioni

The sequence AAV59405 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC119291 Genomic DNA. Translation: AAV59405.1. Sequence problems.
AP008211 Genomic DNA. Translation: BAF18086.1.
AP014961 Genomic DNA. Translation: BAS95102.1.
CM000142 Genomic DNA. Translation: EEE64524.1.
AK068572 mRNA. Translation: BAG90972.1.
RefSeqiXP_015639206.1. XM_015783720.1.
UniGeneiOs.14570.

Genome annotation databases

EnsemblPlantsiOS05T0539400-01; OS05T0539400-01; OS05G0539400.
GeneIDi4339457.
GrameneiOS05T0539400-01; OS05T0539400-01; OS05G0539400.
KEGGiosa:4339457.

Similar proteinsi

Entry informationi

Entry nameiBGAL8_ORYSJ
AccessioniPrimary (citable) accession number: Q0DGD7
Secondary accession number(s): B7EF25, Q53WK7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: October 17, 2006
Last modified: November 22, 2017
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families