Q0DEC8 (SSY1_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Soluble starch synthase 1, chloroplastic/amyloplastic
EC=2.4.1.21

Alternative name(s):
SSS 1
Starch synthase I

Gene names

Ordered Locus Names:	Os06g0160700, LOC_Os06g06560
ORF Names:	P0681F10.13

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	641 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ADP-glucose + (1,4-alpha-D-glucosyl)(n) = ADP + (1,4-alpha-D-glucosyl)(n+1).
Pathway	Glycan biosynthesis; starch biosynthesis.
Subcellular location	Plastid › chloroplast. Plastid › amyloplast. Note: Amyloplast or chloroplast, soluble.
Tissue specificity	Leaves and immature seeds.
Miscellaneous	Three forms of soluble starch synthase were purified: RSS1, RSS2 and RSS3.
Sequence similarities	Belongs to the glycosyltransferase 1 family. Bacterial/plant glycogen synthase subfamily.
Sequence caution	The sequence AAD49850.1 differs from that shown. Reason: Frameshift at position 587. The sequence BAA03739.1 differs from that shown. Reason: Frameshift at position 587. The sequence BAA07396.1 differs from that shown. Reason: Frameshift at position 587.

Ontologies

Keywords
Biological process	Starch biosynthesis
Cellular component	Amyloplast Chloroplast Plastid
Domain	Transit peptide
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	amylopectin biosynthetic process Inferred from electronic annotation. Source: EnsemblPlants/Gramene starch biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	amyloplast Inferred from electronic annotation. Source: UniProtKB-SubCell chloroplast stroma Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	starch synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 113	113	Chloroplast Ref.1
	Chain	114 – 641	528	Soluble starch synthase 1, chloroplastic/amyloplastic		PRO_0000011138
Sites
	Binding site	147	1	ADP-glucose By similarity
Experimental info
	Sequence conflict	46 – 47	2	QR → HG in BAA03739. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q0DEC8 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: EF851A03C7349076	FASTA	641	70,952
	10 20 30 40 50 60 MATAAGMGIG AACLVAPQVR PGRRLRLQRV RRRCVAELSR DGGSAQRPLA PAPLVKQPVL 70 80 90 100 110 120 PTFLVPTSTP PAPTQSPAPA PTPPPLPDSG VGEIEPDLEG LTEDSIDKTI FVASEQESEI 130 140 150 160 170 180 MDVKEQAQAK VTRSVVFVTG EASPYAKSGG LGDVCGSLPI ALALRGHRVM VVMPRYMNGA 190 200 210 220 230 240 LNKNFANAFY TEKHIKIPCF GGEHEVTFFH EYRDSVDWVF VDHPSYHRPG NLYGDNFGAF 250 260 270 280 290 300 GDNQFRYTLL CYAACEAPLI LELGGYIYGQ KCMFVVNDWH ASLVPVLLAA KYRPYGVYRD 310 320 330 340 350 360 ARSVLVIHNL AHQGVEPAST YPDLGLPPEW YGALEWVFPE WARRHALDKG EAVNFLKGAV 370 380 390 400 410 420 VTADRIVTVS QGYSWEVTTA EGGQGLNELL SSRKSVLNGI VNGIDINDWN PSTDKFLPYH 430 440 450 460 470 480 YSVDDLSGKA KCKAELQKEL GLPIRPDVPL IGFIGRLDYQ KGIDLIKLAI PDLMRDNIQF 490 500 510 520 530 540 VMLGSGDPGF EGWMRSTESG YRDKFRGWVG FSVPVSHRIT AGCDILLMPS RFEPCGLNQL 550 560 570 580 590 600 YAMQYGTVPV VHGTGGLRDT VENFNPFAEK GEQGTGWAFS PLTIEKMLWA LRMAISTYRE 610 620 630 640 HKSSWEGLMK RGMSSDFTWD HAASQYEQIF EWAFMDQPYV M

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification, cDNA cloning, and gene expression of soluble starch synthase in rice (Oryza sativa L.) immature seeds." Baba T., Nishihara M., Mizuno K., Kawasaki T., Shimada H., Kobayashi E., Ohnishi S., Tanaka K., Arai Y. Plant Physiol. 103:565-573(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 114-131. Tissue: Seed.
[2]	"Structure, organization and chromosomal location of the gene encoding a form of rice soluble starch synthase." Baba T., Ohnishi S., Tanaka K., Kishimoto N., Kawasaki T. Plant Physiol. 108:677-683(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	Junwang X., Zhen Z. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Nanjing 37.
[4]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[5]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D16202 mRNA. Translation: BAA03739.1. Frameshift. D38221 Genomic DNA. Translation: BAA07396.1. Frameshift. AF165890 mRNA. Translation: AAD49850.1. Frameshift. AB026295 Genomic DNA. Translation: BAD67625.1. AP008212 Genomic DNA. Translation: BAF18795.1.
PIR	JQ2322.
3D structure databases
ProteinModelPortal	Q0DEC8.
ModBase	Search...
Protein family/group databases
CAZy	GT5. Glycosyltransferase Family 5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os06g06560.1; LOC_Os06g06560.1; LOC_Os06g06560.
Organism-specific databases
Gramene	Q0DEC8.
Phylogenomic databases
eggNOG	COG0297.
OMA	FDTVGWA.
Enzyme and pathway databases
UniPathway	UPA00152.
Gene expression databases
ArrayExpress	Q0DEC8.
Family and domain databases
InterPro	IPR001296. Glyco_trans_1. IPR011835. Glycogen/starch_synth. IPR013534. Starch_synth_cat_dom. [Graphical view]
Pfam	PF08323. Glyco_transf_5. 1 hit. PF00534. Glycos_transf_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR02095. glgA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SSY1_ORYSJ

Accession

Primary (citable) accession number: Q0DEC8
Secondary accession number(s): O24206 , Q40739, Q5WA90, Q7Y044

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	October 17, 2006
Last modified:	April 3, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program