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Q0DDE3

- SSY23_ORYSJ

UniProt

Q0DDE3 - SSY23_ORYSJ

Protein

Soluble starch synthase 2-3, chloroplastic/amyloplastic

Gene

SSII-3

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 1 (17 Oct 2006)
      Previous versions | rss
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    Functioni

    Plays an important role during endosperm starch synthesis. Determines the type of amylopectin structure of starch grain. Synthesizes long B1 amylopectin chains by elongating short A and B1 chains, independently of the other soluble starch synthases. Barely active in japonica subspecies.2 Publications

    Catalytic activityi

    ADP-glucose + (1,4-alpha-D-glucosyl)(n) = ADP + (1,4-alpha-D-glucosyl)(n+1).

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei333 – 3331ADP-glucoseBy similarity

    GO - Molecular functioni

    1. starch synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. starch biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Starch biosynthesis

    Enzyme and pathway databases

    UniPathwayiUPA00152.

    Protein family/group databases

    CAZyiGT5. Glycosyltransferase Family 5.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Soluble starch synthase 2-3, chloroplastic/amyloplastic (EC:2.4.1.21)
    Alternative name(s):
    Soluble starch synthase II-3
    Starch synthase IIa
    Gene namesi
    Name:SSII-3
    Synonyms:ALK
    Ordered Locus Names:Os06g0229800, LOC_Os06g12450
    ORF Names:P0525F01.23
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 6

    Organism-specific databases

    GrameneiQ0DDE3.

    Subcellular locationi

    Plastidamyloplast. Plastidchloroplast
    Note: Amyloplast or chloroplast, granule-bound and soluble.

    GO - Cellular componenti

    1. amyloplast Source: UniProtKB-SubCell
    2. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Amyloplast, Chloroplast, Plastid

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi88 – 881D → E: Increased activity; when associated with V-737. 1 Publication
    Mutagenesisi604 – 6041S → G: Increased activity; when associated with V-737. Slightly increased activity; when associated with V-737 and F-781. 1 Publication
    Mutagenesisi737 – 7371M → V: Increased activity. Increased activity; when associated with E-88 and/or G-604. Slightly increased activity; when associated with G-604 and F-781. 1 Publication
    Mutagenesisi781 – 7811L → F: Slightly increased activity; when associated with G-604 and V-737. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 1616ChloroplastSequence AnalysisAdd
    BLAST
    Chaini17 – 810794Soluble starch synthase 2-3, chloroplastic/amyloplasticPRO_0000011141Add
    BLAST

    Proteomic databases

    PRIDEiQ0DDE3.

    Expressioni

    Tissue specificityi

    Expressed most exclusively in endosperm.2 Publications

    Developmental stagei

    Expressed in developing caryopsis at 5 to 15 days after flowering. Expressed exclusively in the endosperm at 5 to 10 days after flowering.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ0DDE3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi53 – 608Poly-Pro
    Compositional biasi178 – 23558Pro-richAdd
    BLAST
    Compositional biasi278 – 30932Asp-richAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0297.
    KOiK00703.
    OMAiITHTETM.

    Family and domain databases

    HAMAPiMF_00484. Glycogen_synth.
    InterProiIPR001296. Glyco_trans_1.
    IPR011835. Glycogen/starch_synth.
    IPR013534. Starch_synth_cat_dom.
    [Graphical view]
    PfamiPF08323. Glyco_transf_5. 1 hit.
    PF00534. Glycos_transf_1. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02095. glgA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q0DDE3-1 [UniParc]FASTAAdd to Basket

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    MSSAVVASST TFLVALASSA SRGGPRRGRV VGVAAPPALL YDGRAGRLAL    50
    RAPPPPRPRP RRRDAGVVRR ADDGENEAAV ERAGEDDDEE EEFSSGAWQP 100
    PRSRRGGVGK VLKRRGTVPP VGRYGSGGDA ARVRGAAAPA PAPTQDAASS 150
    KNGALLSGRD DDTPASRNGS VVTGADKPAA ATPPVTITKL PAPDSPVILP 200
    SVDKPQPEFV IPDATAPAPP PPGSNPRSSA PLPKPDNSEF AEDKSAKVVE 250
    SAPKPKATRS SPIPAVEEET WDFKKYFDLN EPDAAEDGDD DDDWADSDAS 300
    DSEIDQDDDS GPLAGENVMN VIVVAAECSP WCKTGGLGDV AGALPKALAR 350
    RGHRVMVVVP RYGDYAEAQD VGIRKYYKAA GQDLEVKYFH AFIDGVDFVF 400
    IDAPLFRHRQ DDIYGGNRQE IMKRMILFCK AAVEVPWHVP CGGVPYGDGN 450
    LVFLANDWHT ALLPVYLKAY YRDNGMMQYT RSVLVIHNIA YQGRGPVDEF 500
    PYMELPEHYL DHFKLYDPVG GEHANIFGAG LKMADRVVTV SPGYLWELKT 550
    TEGGWGLHDI IRENDWKMNG IVNGIDYREW NPEVDVHLQS DGYANYTVAS 600
    LDSSKPRCKA ALQRELGLEV RDDVPLIGFI GRLDGQKGVD IIGDAMPWIA 650
    GQDVQLVLLG SGRRDLEVML QRFEAQHNSK VRGWVGFSVK MAHRITAGAD 700
    VLVMPSRFEP CGLNQLYAMA YGTVPVVHAV GGLRDTMSAF DPFEDTGLGW 750
    TFDRAEPHKL IEALGHCLET YRKYKESWRG LQVRGMSQDL SWDHAAELYE 800
    EVLVKAKYQW 810
    Length:810
    Mass (Da):88,375
    Last modified:October 17, 2006 - v1
    Checksum:i9676434321EAFE72
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti737 – 7371M → V in strain: cv. Kinmaze. 1 Publication
    Natural varianti781 – 7811L → F in strain: cv. Kinmaze. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF419099 mRNA. Translation: AAL16661.1.
    AB115915 mRNA. Translation: BAD90591.1.
    AB115916 mRNA. Translation: BAD90592.1.
    AP003509 Genomic DNA. Translation: BAD37272.1.
    AP008212 Genomic DNA. Translation: BAF19130.1.
    RefSeqiNP_001057216.1. NM_001063751.1.
    UniGeneiOs.5738.

    Genome annotation databases

    EnsemblPlantsiOS06T0229800-01; OS06T0229800-01; OS06G0229800.
    GeneIDi4340567.
    KEGGiosa:4340567.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF419099 mRNA. Translation: AAL16661.1 .
    AB115915 mRNA. Translation: BAD90591.1 .
    AB115916 mRNA. Translation: BAD90592.1 .
    AP003509 Genomic DNA. Translation: BAD37272.1 .
    AP008212 Genomic DNA. Translation: BAF19130.1 .
    RefSeqi NP_001057216.1. NM_001063751.1.
    UniGenei Os.5738.

    3D structure databases

    ProteinModelPortali Q0DDE3.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GT5. Glycosyltransferase Family 5.

    Proteomic databases

    PRIDEi Q0DDE3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS06T0229800-01 ; OS06T0229800-01 ; OS06G0229800 .
    GeneIDi 4340567.
    KEGGi osa:4340567.

    Organism-specific databases

    Gramenei Q0DDE3.

    Phylogenomic databases

    eggNOGi COG0297.
    KOi K00703.
    OMAi ITHTETM.

    Enzyme and pathway databases

    UniPathwayi UPA00152 .

    Family and domain databases

    HAMAPi MF_00484. Glycogen_synth.
    InterProi IPR001296. Glyco_trans_1.
    IPR011835. Glycogen/starch_synth.
    IPR013534. Starch_synth_cat_dom.
    [Graphical view ]
    Pfami PF08323. Glyco_transf_5. 1 hit.
    PF00534. Glycos_transf_1. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02095. glgA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and expression analysis of three genes encoding starch synthase II in rice."
      Jiang H.W., Dian W.M., Liu F., Wu P.
      Planta 218:1062-1070(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 71-78, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    2. "Essential amino acids of starch synthase IIa differentiate amylopectin structure and starch quality between japonica and indica rice varieties."
      Nakamura Y., Francisco P.B. Jr., Hosaka Y., Sato A., Sawada T., Kubo A., Fujita N.
      Plant Mol. Biol. 58:213-227(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, VARIANTS VAL-737 AND PHE-781, MUTAGENESIS OF ASP-88; SER-604; MET-737 AND LEU-781.
      Strain: cv. Kinmaze and cv. Nipponbare.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    4. "The rice annotation project database (RAP-DB): 2008 update."
      The rice annotation project (RAP)
      Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: cv. Nipponbare.
    5. "A comprehensive expression analysis of the starch synthase gene family in rice (Oryza sativa L.)."
      Hirose T., Terao T.
      Planta 220:9-16(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, NOMENCLATURE.

    Entry informationi

    Entry nameiSSY23_ORYSJ
    AccessioniPrimary (citable) accession number: Q0DDE3
    Secondary accession number(s): Q5DWW9
    , Q5DWX1, Q67X47, Q6TDS3, Q944W5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 24, 2007
    Last sequence update: October 17, 2006
    Last modified: October 1, 2014
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Increased activity due to variations in subspecies indica is associated with the synthesis of the L-type amylopectin, which contains much lower proportion of short amylopectin chains than the S-type amylopectin found in most japonica varieties. The difference in amylopectin chain composition explains why starch from japonica subspecies has a lower gelatinisation temperature than starch from indica subspecies.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3