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Q0DDE3

- SSY23_ORYSJ

UniProt

Q0DDE3 - SSY23_ORYSJ

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Protein

Soluble starch synthase 2-3, chloroplastic/amyloplastic

Gene

SSII-3

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Plays an important role during endosperm starch synthesis. Determines the type of amylopectin structure of starch grain. Synthesizes long B1 amylopectin chains by elongating short A and B1 chains, independently of the other soluble starch synthases. Barely active in japonica subspecies.2 Publications

Catalytic activityi

ADP-glucose + (1,4-alpha-D-glucosyl)(n) = ADP + (1,4-alpha-D-glucosyl)(n+1).

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei333 – 3331ADP-glucoseBy similarity

GO - Molecular functioni

  1. starch synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. starch biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Starch biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00152.

Protein family/group databases

CAZyiGT5. Glycosyltransferase Family 5.

Names & Taxonomyi

Protein namesi
Recommended name:
Soluble starch synthase 2-3, chloroplastic/amyloplastic (EC:2.4.1.21)
Alternative name(s):
Soluble starch synthase II-3
Starch synthase IIa
Gene namesi
Name:SSII-3
Synonyms:ALK
Ordered Locus Names:Os06g0229800, LOC_Os06g12450
ORF Names:P0525F01.23
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 6

Organism-specific databases

GrameneiQ0DDE3.

Subcellular locationi

Plastidamyloplast. Plastidchloroplast
Note: Amyloplast or chloroplast, granule-bound and soluble.

GO - Cellular componenti

  1. amyloplast Source: UniProtKB-KW
  2. chloroplast Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Amyloplast, Chloroplast, Plastid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi88 – 881D → E: Increased activity; when associated with V-737. 1 Publication
Mutagenesisi604 – 6041S → G: Increased activity; when associated with V-737. Slightly increased activity; when associated with V-737 and F-781. 1 Publication
Mutagenesisi737 – 7371M → V: Increased activity. Increased activity; when associated with E-88 and/or G-604. Slightly increased activity; when associated with G-604 and F-781. 1 Publication
Mutagenesisi781 – 7811L → F: Slightly increased activity; when associated with G-604 and V-737. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 1616ChloroplastSequence AnalysisAdd
BLAST
Chaini17 – 810794Soluble starch synthase 2-3, chloroplastic/amyloplasticPRO_0000011141Add
BLAST

Proteomic databases

PRIDEiQ0DDE3.

Expressioni

Tissue specificityi

Expressed most exclusively in endosperm.2 Publications

Developmental stagei

Expressed in developing caryopsis at 5 to 15 days after flowering. Expressed exclusively in the endosperm at 5 to 10 days after flowering.1 Publication

Gene expression databases

ExpressionAtlasiQ0DDE3. baseline.

Structurei

3D structure databases

ProteinModelPortaliQ0DDE3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi53 – 608Poly-Pro
Compositional biasi178 – 23558Pro-richAdd
BLAST
Compositional biasi278 – 30932Asp-richAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0297.
InParanoidiQ0DDE3.
KOiK00703.
OMAiITHTETM.

Family and domain databases

HAMAPiMF_00484. Glycogen_synth.
InterProiIPR001296. Glyco_trans_1.
IPR011835. Glycogen/starch_synth.
IPR013534. Starch_synth_cat_dom.
[Graphical view]
PfamiPF08323. Glyco_transf_5. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02095. glgA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0DDE3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSAVVASST TFLVALASSA SRGGPRRGRV VGVAAPPALL YDGRAGRLAL
60 70 80 90 100
RAPPPPRPRP RRRDAGVVRR ADDGENEAAV ERAGEDDDEE EEFSSGAWQP
110 120 130 140 150
PRSRRGGVGK VLKRRGTVPP VGRYGSGGDA ARVRGAAAPA PAPTQDAASS
160 170 180 190 200
KNGALLSGRD DDTPASRNGS VVTGADKPAA ATPPVTITKL PAPDSPVILP
210 220 230 240 250
SVDKPQPEFV IPDATAPAPP PPGSNPRSSA PLPKPDNSEF AEDKSAKVVE
260 270 280 290 300
SAPKPKATRS SPIPAVEEET WDFKKYFDLN EPDAAEDGDD DDDWADSDAS
310 320 330 340 350
DSEIDQDDDS GPLAGENVMN VIVVAAECSP WCKTGGLGDV AGALPKALAR
360 370 380 390 400
RGHRVMVVVP RYGDYAEAQD VGIRKYYKAA GQDLEVKYFH AFIDGVDFVF
410 420 430 440 450
IDAPLFRHRQ DDIYGGNRQE IMKRMILFCK AAVEVPWHVP CGGVPYGDGN
460 470 480 490 500
LVFLANDWHT ALLPVYLKAY YRDNGMMQYT RSVLVIHNIA YQGRGPVDEF
510 520 530 540 550
PYMELPEHYL DHFKLYDPVG GEHANIFGAG LKMADRVVTV SPGYLWELKT
560 570 580 590 600
TEGGWGLHDI IRENDWKMNG IVNGIDYREW NPEVDVHLQS DGYANYTVAS
610 620 630 640 650
LDSSKPRCKA ALQRELGLEV RDDVPLIGFI GRLDGQKGVD IIGDAMPWIA
660 670 680 690 700
GQDVQLVLLG SGRRDLEVML QRFEAQHNSK VRGWVGFSVK MAHRITAGAD
710 720 730 740 750
VLVMPSRFEP CGLNQLYAMA YGTVPVVHAV GGLRDTMSAF DPFEDTGLGW
760 770 780 790 800
TFDRAEPHKL IEALGHCLET YRKYKESWRG LQVRGMSQDL SWDHAAELYE
810
EVLVKAKYQW
Length:810
Mass (Da):88,375
Last modified:October 17, 2006 - v1
Checksum:i9676434321EAFE72
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti737 – 7371M → V in strain: cv. Kinmaze. 1 Publication
Natural varianti781 – 7811L → F in strain: cv. Kinmaze. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF419099 mRNA. Translation: AAL16661.1.
AB115915 mRNA. Translation: BAD90591.1.
AB115916 mRNA. Translation: BAD90592.1.
AP003509 Genomic DNA. Translation: BAD37272.1.
AP008212 Genomic DNA. Translation: BAF19130.1.
RefSeqiNP_001057216.1. NM_001063751.1.
UniGeneiOs.5738.

Genome annotation databases

EnsemblPlantsiOS06T0229800-01; OS06T0229800-01; OS06G0229800.
GeneIDi4340567.
KEGGiosa:4340567.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF419099 mRNA. Translation: AAL16661.1 .
AB115915 mRNA. Translation: BAD90591.1 .
AB115916 mRNA. Translation: BAD90592.1 .
AP003509 Genomic DNA. Translation: BAD37272.1 .
AP008212 Genomic DNA. Translation: BAF19130.1 .
RefSeqi NP_001057216.1. NM_001063751.1.
UniGenei Os.5738.

3D structure databases

ProteinModelPortali Q0DDE3.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GT5. Glycosyltransferase Family 5.

Proteomic databases

PRIDEi Q0DDE3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS06T0229800-01 ; OS06T0229800-01 ; OS06G0229800 .
GeneIDi 4340567.
KEGGi osa:4340567.

Organism-specific databases

Gramenei Q0DDE3.

Phylogenomic databases

eggNOGi COG0297.
InParanoidi Q0DDE3.
KOi K00703.
OMAi ITHTETM.

Enzyme and pathway databases

UniPathwayi UPA00152 .

Gene expression databases

ExpressionAtlasi Q0DDE3. baseline.

Family and domain databases

HAMAPi MF_00484. Glycogen_synth.
InterProi IPR001296. Glyco_trans_1.
IPR011835. Glycogen/starch_synth.
IPR013534. Starch_synth_cat_dom.
[Graphical view ]
Pfami PF08323. Glyco_transf_5. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02095. glgA. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and expression analysis of three genes encoding starch synthase II in rice."
    Jiang H.W., Dian W.M., Liu F., Wu P.
    Planta 218:1062-1070(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 71-78, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  2. "Essential amino acids of starch synthase IIa differentiate amylopectin structure and starch quality between japonica and indica rice varieties."
    Nakamura Y., Francisco P.B. Jr., Hosaka Y., Sato A., Sawada T., Kubo A., Fujita N.
    Plant Mol. Biol. 58:213-227(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, VARIANTS VAL-737 AND PHE-781, MUTAGENESIS OF ASP-88; SER-604; MET-737 AND LEU-781.
    Strain: cv. Kinmaze and cv. Nipponbare.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  4. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  5. "A comprehensive expression analysis of the starch synthase gene family in rice (Oryza sativa L.)."
    Hirose T., Terao T.
    Planta 220:9-16(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, NOMENCLATURE.

Entry informationi

Entry nameiSSY23_ORYSJ
AccessioniPrimary (citable) accession number: Q0DDE3
Secondary accession number(s): Q5DWW9
, Q5DWX1, Q67X47, Q6TDS3, Q944W5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: October 17, 2006
Last modified: October 29, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Increased activity due to variations in subspecies indica is associated with the synthesis of the L-type amylopectin, which contains much lower proportion of short amylopectin chains than the S-type amylopectin found in most japonica varieties. The difference in amylopectin chain composition explains why starch from japonica subspecies has a lower gelatinisation temperature than starch from indica subspecies.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3