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Q0DDE3 (SSY23_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Soluble starch synthase 2-3, chloroplastic/amyloplastic

EC=2.4.1.21
Alternative name(s):
Soluble starch synthase II-3
Starch synthase IIa
Gene names
Name:SSII-3
Synonyms:ALK
Ordered Locus Names:Os06g0229800, LOC_Os06g12450
ORF Names:P0525F01.23
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length810 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays an important role during endosperm starch synthesis. Determines the type of amylopectin structure of starch grain. Synthesizes long B1 amylopectin chains by elongating short A and B1 chains, independently of the other soluble starch synthases. Barely active in japonica subspecies. Ref.1 Ref.2

Catalytic activity

ADP-glucose + (1,4-alpha-D-glucosyl)(n) = ADP + (1,4-alpha-D-glucosyl)(n+1). HAMAP-Rule MF_00484

Pathway

Glycan biosynthesis; starch biosynthesis. HAMAP-Rule MF_00484

Subcellular location

Plastidamyloplast. Plastidchloroplast. Note: Amyloplast or chloroplast, granule-bound and soluble. Ref.1 Ref.5

Tissue specificity

Expressed most exclusively in endosperm. Ref.1 Ref.5

Developmental stage

Expressed in developing caryopsis at 5 to 15 days after flowering. Expressed exclusively in the endosperm at 5 to 10 days after flowering. Ref.1

Miscellaneous

Increased activity due to variations in subspecies indica is associated with the synthesis of the L-type amylopectin, which contains much lower proportion of short amylopectin chains than the S-type amylopectin found in most japonica varieties. The difference in amylopectin chain composition explains why starch from japonica subspecies has a lower gelatinisation temperature than starch from indica subspecies.

Sequence similarities

Belongs to the glycosyltransferase 1 family. Bacterial/plant glycogen synthase subfamily.

Ontologies

Keywords
   Biological processStarch biosynthesis
   Cellular componentAmyloplast
Chloroplast
Plastid
   DomainTransit peptide
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processstarch biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentamyloplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

chloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionstarch synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 1616Chloroplast Potential
Chain17 – 810794Soluble starch synthase 2-3, chloroplastic/amyloplastic HAMAP-Rule MF_00484
PRO_0000011141

Regions

Compositional bias53 – 608Poly-Pro HAMAP-Rule MF_00484
Compositional bias178 – 23558Pro-rich HAMAP-Rule MF_00484
Compositional bias278 – 30932Asp-rich HAMAP-Rule MF_00484

Sites

Binding site3331ADP-glucose By similarity

Natural variations

Natural variant7371M → V in strain: cv. Kinmaze. Ref.2
Natural variant7811L → F in strain: cv. Kinmaze. Ref.2

Experimental info

Mutagenesis881D → E: Increased activity; when associated with V-737. Ref.2
Mutagenesis6041S → G: Increased activity; when associated with V-737. Slightly increased activity; when associated with V-737 and F-781. Ref.2
Mutagenesis7371M → V: Increased activity. Increased activity; when associated with E-88 and/or G-604. Slightly increased activity; when associated with G-604 and F-781. Ref.2
Mutagenesis7811L → F: Slightly increased activity; when associated with G-604 and V-737. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q0DDE3 [UniParc].

Last modified October 17, 2006. Version 1.
Checksum: 9676434321EAFE72

FASTA81088,375
        10         20         30         40         50         60 
MSSAVVASST TFLVALASSA SRGGPRRGRV VGVAAPPALL YDGRAGRLAL RAPPPPRPRP 

        70         80         90        100        110        120 
RRRDAGVVRR ADDGENEAAV ERAGEDDDEE EEFSSGAWQP PRSRRGGVGK VLKRRGTVPP 

       130        140        150        160        170        180 
VGRYGSGGDA ARVRGAAAPA PAPTQDAASS KNGALLSGRD DDTPASRNGS VVTGADKPAA 

       190        200        210        220        230        240 
ATPPVTITKL PAPDSPVILP SVDKPQPEFV IPDATAPAPP PPGSNPRSSA PLPKPDNSEF 

       250        260        270        280        290        300 
AEDKSAKVVE SAPKPKATRS SPIPAVEEET WDFKKYFDLN EPDAAEDGDD DDDWADSDAS 

       310        320        330        340        350        360 
DSEIDQDDDS GPLAGENVMN VIVVAAECSP WCKTGGLGDV AGALPKALAR RGHRVMVVVP 

       370        380        390        400        410        420 
RYGDYAEAQD VGIRKYYKAA GQDLEVKYFH AFIDGVDFVF IDAPLFRHRQ DDIYGGNRQE 

       430        440        450        460        470        480 
IMKRMILFCK AAVEVPWHVP CGGVPYGDGN LVFLANDWHT ALLPVYLKAY YRDNGMMQYT 

       490        500        510        520        530        540 
RSVLVIHNIA YQGRGPVDEF PYMELPEHYL DHFKLYDPVG GEHANIFGAG LKMADRVVTV 

       550        560        570        580        590        600 
SPGYLWELKT TEGGWGLHDI IRENDWKMNG IVNGIDYREW NPEVDVHLQS DGYANYTVAS 

       610        620        630        640        650        660 
LDSSKPRCKA ALQRELGLEV RDDVPLIGFI GRLDGQKGVD IIGDAMPWIA GQDVQLVLLG 

       670        680        690        700        710        720 
SGRRDLEVML QRFEAQHNSK VRGWVGFSVK MAHRITAGAD VLVMPSRFEP CGLNQLYAMA 

       730        740        750        760        770        780 
YGTVPVVHAV GGLRDTMSAF DPFEDTGLGW TFDRAEPHKL IEALGHCLET YRKYKESWRG 

       790        800        810 
LQVRGMSQDL SWDHAAELYE EVLVKAKYQW 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and expression analysis of three genes encoding starch synthase II in rice."
Jiang H.W., Dian W.M., Liu F., Wu P.
Planta 218:1062-1070(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 71-78, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[2]"Essential amino acids of starch synthase IIa differentiate amylopectin structure and starch quality between japonica and indica rice varieties."
Nakamura Y., Francisco P.B. Jr., Hosaka Y., Sato A., Sawada T., Kubo A., Fujita N.
Plant Mol. Biol. 58:213-227(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, VARIANTS VAL-737 AND PHE-781, MUTAGENESIS OF ASP-88; SER-604; MET-737 AND LEU-781.
Strain: cv. Kinmaze and cv. Nipponbare.
[3]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[4]"The rice annotation project database (RAP-DB): 2008 update."
The rice annotation project (RAP)
Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[5]"A comprehensive expression analysis of the starch synthase gene family in rice (Oryza sativa L.)."
Hirose T., Terao T.
Planta 220:9-16(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, NOMENCLATURE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF419099 mRNA. Translation: AAL16661.1.
AB115915 mRNA. Translation: BAD90591.1.
AB115916 mRNA. Translation: BAD90592.1.
AP003509 Genomic DNA. Translation: BAD37272.1.
AP008212 Genomic DNA. Translation: BAF19130.1.
RefSeqNP_001057216.1. NM_001063751.1.
UniGeneOs.5738.

3D structure databases

ProteinModelPortalQ0DDE3.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGT5. Glycosyltransferase Family 5.

Proteomic databases

PRIDEQ0DDE3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS06T0229800-01; OS06T0229800-01; OS06G0229800.
GeneID4340567.
KEGGosa:4340567.

Organism-specific databases

GrameneQ0DDE3.

Phylogenomic databases

eggNOGCOG0297.
KOK00703.
OMAERDINSY.
ProtClustDBCLSN2696078.

Enzyme and pathway databases

UniPathwayUPA00152.

Family and domain databases

HAMAPMF_00484. Glycogen_synth.
InterProIPR001296. Glyco_trans_1.
IPR011835. Glycogen/starch_synth.
IPR013534. Starch_synth_cat_dom.
[Graphical view]
PfamPF08323. Glyco_transf_5. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsTIGR02095. glgA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSSY23_ORYSJ
AccessionPrimary (citable) accession number: Q0DDE3
Secondary accession number(s): Q5DWW9 expand/collapse secondary AC list , Q5DWX1, Q67X47, Q6TDS3, Q944W5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: October 17, 2006
Last modified: April 16, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

PATHWAY comments

Index of metabolic and biosynthesis pathways