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Q0DA21

- BGL25_ORYSJ

UniProt

Q0DA21 - BGL25_ORYSJ

Protein

Beta-glucosidase 25

Gene

BGLU25

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 55 (01 Oct 2014)
      Sequence version 2 (15 Dec 2009)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei39 – 391SubstrateBy similarity
    Binding sitei140 – 1401SubstrateBy similarity
    Binding sitei185 – 1851SubstrateBy similarity
    Active sitei186 – 1861Proton donorBy similarity
    Binding sitei329 – 3291SubstrateBy similarity
    Active sitei402 – 4021NucleophileBy similarity
    Binding sitei452 – 4521SubstrateBy similarity

    GO - Molecular functioni

    1. beta-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-glucosidase 25 (EC:3.2.1.21)
    Short name:
    Os6bglu25
    Gene namesi
    Name:BGLU25
    Ordered Locus Names:Os06g0683300, LOC_Os06g46940
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 6

    Organism-specific databases

    GrameneiQ0DA21.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 501482Beta-glucosidase 25PRO_0000390342Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi107 – 1071N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi205 ↔ 213By similarity
    Glycosylationi478 – 4781N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiQ0DA21.

    Interactioni

    Protein-protein interaction databases

    STRINGi39947.LOC_Os06g46940.2.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0DA21.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni459 – 4602Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2723.
    HOGENOMiHOG000088630.
    KOiK01188.
    OMAiSSWLHIV.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q0DA21-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSLLTLVHIL VSFSACVEAI SRADFPPGFI FGTASSAYQY EGAVNEGQRG    50
    PTIWDTLTKR PGRVIDFSNA DVAVDHYHRY KEDVELMNDI GMDAYRFSIS 100
    WSRIFPNGTG EPNEEGLSYY NSLIDALLDK GIEPYVTLFH WDLPQALEDR 150
    YGGWLNSEII EDFVQYAFTC FKEFGDRVKH WITFNEPYNF AIDGYDLGIQ 200
    APGRCSILSH VFCREGKSST EPYIVAHNIL LAHAGAFRAY EQHFKNEQGG 250
    LIGIALNSRW YEPFSNADED TEAAARAMDF ELGWFLDPLM FGHYPPSMQK 300
    LAGDRLPQFS THASKLVSGS LDFVGINHYT TLYARNDRLR IRKLVMDDAS 350
    TDSAVIPTAY RHGKKIGETA ASSWLHIVPW GMFKLMKHVK EKYGNPPVVI 400
    TENGMDDANH PFSRLEDVLQ DDKRIQYHND YMSNLLDAIR KEGCNVHGYF 450
    VWSLLDNWEW NSGYTVRFGL YYIDYKNNLT RIPKASVQWF SQVLAQKTAI 500
    I 501
    Length:501
    Mass (Da):57,191
    Last modified:December 15, 2009 - v2
    Checksum:i09FEA57303269054
    GO
    Isoform 2 (identifier: Q0DA21-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-86: Missing.
         303-312: GDRLPQFSTH → ETIDCGSGNL
         313-501: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:226
    Mass (Da):25,712
    Checksum:i8CA8C0A5987DD9E7
    GO

    Sequence cautioni

    The sequence BAF20302.1 differs from that shown. Reason: Erroneous gene model prediction.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 8686Missing in isoform 2. 1 PublicationVSP_038508Add
    BLAST
    Alternative sequencei303 – 31210GDRLPQFSTH → ETIDCGSGNL in isoform 2. 1 PublicationVSP_038509
    Alternative sequencei313 – 501189Missing in isoform 2. 1 PublicationVSP_038510Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008212 Genomic DNA. Translation: BAF20302.1. Sequence problems.
    AK068614 mRNA. Translation: BAG90993.1.
    AK120488 mRNA. Translation: BAH00037.1.
    RefSeqiNP_001058388.1. NM_001064923.1.
    UniGeneiOs.49601.

    Genome annotation databases

    GeneIDi4341870.
    KEGGiosa:4341870.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008212 Genomic DNA. Translation: BAF20302.1 . Sequence problems.
    AK068614 mRNA. Translation: BAG90993.1 .
    AK120488 mRNA. Translation: BAH00037.1 .
    RefSeqi NP_001058388.1. NM_001064923.1.
    UniGenei Os.49601.

    3D structure databases

    ProteinModelPortali Q0DA21.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 39947.LOC_Os06g46940.2.

    Protein family/group databases

    CAZyi GH1. Glycoside Hydrolase Family 1.

    Proteomic databases

    PRIDEi Q0DA21.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 4341870.
    KEGGi osa:4341870.

    Organism-specific databases

    Gramenei Q0DA21.

    Phylogenomic databases

    eggNOGi COG2723.
    HOGENOMi HOG000088630.
    KOi K01188.
    OMAi SSWLHIV.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10353. PTHR10353. 1 hit.
    Pfami PF00232. Glyco_hydro_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00131. GLHYDRLASE1.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    2. "The rice annotation project database (RAP-DB): 2008 update."
      The rice annotation project (RAP)
      Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: cv. Nipponbare.
    3. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
      The rice full-length cDNA consortium
      Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: cv. Nipponbare.
    4. "Analysis of rice glycosyl hydrolase family 1 and expression of Os4bglu12 beta-glucosidase."
      Opassiri R., Pomthong B., Onkoksoong T., Akiyama T., Esen A., Ketudat Cairns J.R.
      BMC Plant Biol. 6:33-33(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiBGL25_ORYSJ
    AccessioniPrimary (citable) accession number: Q0DA21
    Secondary accession number(s): B7EF46
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 2009
    Last sequence update: December 15, 2009
    Last modified: October 1, 2014
    This is version 55 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3