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Protein

Beta-glucosidase 25

Gene

BGLU25

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei39SubstrateBy similarity1
Binding sitei140SubstrateBy similarity1
Binding sitei185SubstrateBy similarity1
Active sitei186Proton donorBy similarity1
Binding sitei329SubstrateBy similarity1
Active sitei402NucleophileBy similarity1
Binding sitei452SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucosidase 25 (EC:3.2.1.21)
Short name:
Os6bglu25
Gene namesi
Name:BGLU25
Ordered Locus Names:Os06g0683300, LOC_Os06g46940
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 6

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000039034220 – 501Beta-glucosidase 25Add BLAST482

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi107N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi205 ↔ 213By similarity
Glycosylationi478N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ0DA21.
PRIDEiQ0DA21.

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os06g46940.2.

Structurei

3D structure databases

ProteinModelPortaliQ0DA21.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni459 – 460Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0626. Eukaryota.
COG2723. LUCA.
HOGENOMiHOG000088630.
InParanoidiQ0DA21.
KOiK01188.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR033132. Glyco_hydro_1_N_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q0DA21-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLLTLVHIL VSFSACVEAI SRADFPPGFI FGTASSAYQY EGAVNEGQRG
60 70 80 90 100
PTIWDTLTKR PGRVIDFSNA DVAVDHYHRY KEDVELMNDI GMDAYRFSIS
110 120 130 140 150
WSRIFPNGTG EPNEEGLSYY NSLIDALLDK GIEPYVTLFH WDLPQALEDR
160 170 180 190 200
YGGWLNSEII EDFVQYAFTC FKEFGDRVKH WITFNEPYNF AIDGYDLGIQ
210 220 230 240 250
APGRCSILSH VFCREGKSST EPYIVAHNIL LAHAGAFRAY EQHFKNEQGG
260 270 280 290 300
LIGIALNSRW YEPFSNADED TEAAARAMDF ELGWFLDPLM FGHYPPSMQK
310 320 330 340 350
LAGDRLPQFS THASKLVSGS LDFVGINHYT TLYARNDRLR IRKLVMDDAS
360 370 380 390 400
TDSAVIPTAY RHGKKIGETA ASSWLHIVPW GMFKLMKHVK EKYGNPPVVI
410 420 430 440 450
TENGMDDANH PFSRLEDVLQ DDKRIQYHND YMSNLLDAIR KEGCNVHGYF
460 470 480 490 500
VWSLLDNWEW NSGYTVRFGL YYIDYKNNLT RIPKASVQWF SQVLAQKTAI

I
Length:501
Mass (Da):57,191
Last modified:December 15, 2009 - v2
Checksum:i09FEA57303269054
GO
Isoform 2 (identifier: Q0DA21-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-86: Missing.
     303-312: GDRLPQFSTH → ETIDCGSGNL
     313-501: Missing.

Note: No experimental confirmation available.
Show »
Length:226
Mass (Da):25,712
Checksum:i8CA8C0A5987DD9E7
GO

Sequence cautioni

The sequence BAF20302 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0385081 – 86Missing in isoform 2. 1 PublicationAdd BLAST86
Alternative sequenceiVSP_038509303 – 312GDRLPQFSTH → ETIDCGSGNL in isoform 2. 1 Publication10
Alternative sequenceiVSP_038510313 – 501Missing in isoform 2. 1 PublicationAdd BLAST189

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008212 Genomic DNA. Translation: BAF20302.1. Sequence problems.
AP014962 Genomic DNA. No translation available.
AK068614 mRNA. Translation: BAG90993.1.
AK120488 mRNA. Translation: BAH00037.1.
RefSeqiXP_015643676.1. XM_015788190.1. [Q0DA21-1]
UniGeneiOs.49601.

Genome annotation databases

GeneIDi4341870.
KEGGiosa:4341870.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008212 Genomic DNA. Translation: BAF20302.1. Sequence problems.
AP014962 Genomic DNA. No translation available.
AK068614 mRNA. Translation: BAG90993.1.
AK120488 mRNA. Translation: BAH00037.1.
RefSeqiXP_015643676.1. XM_015788190.1. [Q0DA21-1]
UniGeneiOs.49601.

3D structure databases

ProteinModelPortaliQ0DA21.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os06g46940.2.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Proteomic databases

PaxDbiQ0DA21.
PRIDEiQ0DA21.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi4341870.
KEGGiosa:4341870.

Phylogenomic databases

eggNOGiKOG0626. Eukaryota.
COG2723. LUCA.
HOGENOMiHOG000088630.
InParanoidiQ0DA21.
KOiK01188.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR033132. Glyco_hydro_1_N_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBGL25_ORYSJ
AccessioniPrimary (citable) accession number: Q0DA21
Secondary accession number(s): B7EF46
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: December 15, 2009
Last modified: October 5, 2016
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.