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Q0DA21 (BGL25_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-glucosidase 25

Short name=Os6bglu25
EC=3.2.1.21
Gene names
Name:BGLU25
Ordered Locus Names:Os06g0683300, LOC_Os06g46940
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length501 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Sequence similarities

Belongs to the glycosyl hydrolase 1 family.

Sequence caution

The sequence BAF20302.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-glucosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q0DA21-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q0DA21-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-86: Missing.
     303-312: GDRLPQFSTH → ETIDCGSGNL
     313-501: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 501482Beta-glucosidase 25
PRO_0000390342

Regions

Region459 – 4602Substrate binding By similarity

Sites

Active site1861Proton donor By similarity
Active site4021Nucleophile By similarity
Binding site391Substrate By similarity
Binding site1401Substrate By similarity
Binding site1851Substrate By similarity
Binding site3291Substrate By similarity
Binding site4521Substrate By similarity

Amino acid modifications

Glycosylation1071N-linked (GlcNAc...) Potential
Glycosylation4781N-linked (GlcNAc...) Potential
Disulfide bond205 ↔ 213 By similarity

Natural variations

Alternative sequence1 – 8686Missing in isoform 2.
VSP_038508
Alternative sequence303 – 31210GDRLPQFSTH → ETIDCGSGNL in isoform 2.
VSP_038509
Alternative sequence313 – 501189Missing in isoform 2.
VSP_038510

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 15, 2009. Version 2.
Checksum: 09FEA57303269054

FASTA50157,191
        10         20         30         40         50         60 
MSLLTLVHIL VSFSACVEAI SRADFPPGFI FGTASSAYQY EGAVNEGQRG PTIWDTLTKR 

        70         80         90        100        110        120 
PGRVIDFSNA DVAVDHYHRY KEDVELMNDI GMDAYRFSIS WSRIFPNGTG EPNEEGLSYY 

       130        140        150        160        170        180 
NSLIDALLDK GIEPYVTLFH WDLPQALEDR YGGWLNSEII EDFVQYAFTC FKEFGDRVKH 

       190        200        210        220        230        240 
WITFNEPYNF AIDGYDLGIQ APGRCSILSH VFCREGKSST EPYIVAHNIL LAHAGAFRAY 

       250        260        270        280        290        300 
EQHFKNEQGG LIGIALNSRW YEPFSNADED TEAAARAMDF ELGWFLDPLM FGHYPPSMQK 

       310        320        330        340        350        360 
LAGDRLPQFS THASKLVSGS LDFVGINHYT TLYARNDRLR IRKLVMDDAS TDSAVIPTAY 

       370        380        390        400        410        420 
RHGKKIGETA ASSWLHIVPW GMFKLMKHVK EKYGNPPVVI TENGMDDANH PFSRLEDVLQ 

       430        440        450        460        470        480 
DDKRIQYHND YMSNLLDAIR KEGCNVHGYF VWSLLDNWEW NSGYTVRFGL YYIDYKNNLT 

       490        500 
RIPKASVQWF SQVLAQKTAI I 

« Hide

Isoform 2 [UniParc].

Checksum: 8CA8C0A5987DD9E7
Show »

FASTA22625,712

References

« Hide 'large scale' references
[1]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[2]"The rice annotation project database (RAP-DB): 2008 update."
The rice annotation project (RAP)
Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[3]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: cv. Nipponbare.
[4]"Analysis of rice glycosyl hydrolase family 1 and expression of Os4bglu12 beta-glucosidase."
Opassiri R., Pomthong B., Onkoksoong T., Akiyama T., Esen A., Ketudat Cairns J.R.
BMC Plant Biol. 6:33-33(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP008212 Genomic DNA. Translation: BAF20302.1. Sequence problems.
AK068614 mRNA. Translation: BAG90993.1.
AK120488 mRNA. Translation: BAH00037.1.
RefSeqNP_001058388.1. NM_001064923.1.
UniGeneOs.49601.

3D structure databases

ProteinModelPortalQ0DA21.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING39947.LOC_Os06g46940.2.

Protein family/group databases

CAZyGH1. Glycoside Hydrolase Family 1.

Proteomic databases

PRIDEQ0DA21.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4341870.
KEGGosa:4341870.

Organism-specific databases

GrameneQ0DA21.

Phylogenomic databases

eggNOGCOG2723.
HOGENOMHOG000088630.
KOK01188.
OMAKSSTEPY.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10353. PTHR10353. 1 hit.
PfamPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSPR00131. GLHYDRLASE1.
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGL25_ORYSJ
AccessionPrimary (citable) accession number: Q0DA21
Secondary accession number(s): B7EF46
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: December 15, 2009
Last modified: April 16, 2014
This is version 53 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries