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Reviewed, UniProtKB/Swiss-Prot Q0D9J1 (AMY2A_ORYSJ)

Last modified September 22, 2009. Version 22. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Alpha-amylase isozyme 2A
    EC=3.2.1.1
Alternative name(s):
    1,4-alpha-D-glucan glucanohydrolase
    Alpha-amylase isozyme C2
Gene names
Name: AMY2A
Synonyms: AMY1.5, AMYC2
Ordered Locus Names: Os06g0713800, LOC_Os06g49970
ORF Names: OJ1136_F03.12
OrganismOryza sativa subsp. japonica (Rice)
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

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Protein attributes

Sequence length445 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Important for breakdown of endosperm starch during germination.

Catalytic activity

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in oligosaccharides and polysaccharides.

Cofactor

Binds 3 calcium ions per subunit By similarity.

Subunit structure

Monomer By similarity.

Developmental stage

Expressed at a high level during germination in the aleurones cells under the control of the plant hormone gibberellic acid and in the developing grains at a low level.

Sequence similarities

Belongs to the glycosyl hydrolase 13 family.

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Ontologies

Keywords
   Biological processCarbohydrate metabolism
   DomainSignal
   LigandCalcium
Metal-binding
   Molecular functionGlycosidase
Hydrolase
Gene Ontology (GO)
   Biological processcarbohydrate metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionalpha-amylase activity

Inferred from electronic annotation. Source: EC

calcium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 445424Alpha-amylase isozyme 2A
PRO_0000001409

Sites

Active site2021Nucleophile By similarity
Active site2281Proton donor By similarity
Active site3141 By similarity
Metal binding1131Calcium 1 By similarity
Metal binding1301Calcium 2 By similarity
Metal binding1331Calcium 2 By similarity
Metal binding1351Calcium 2 By similarity
Metal binding1391Calcium 2 By similarity
Metal binding1491Calcium 3 By similarity
Metal binding1601Calcium 3 By similarity
Metal binding1631Calcium 3; via carbonyl oxygen By similarity
Metal binding1641Calcium 1 By similarity
Metal binding1651Calcium 1; via carbonyl oxygen By similarity
Metal binding1681Calcium 3; via carbonyl oxygen By similarity
Metal binding1701Calcium 1 By similarity
Metal binding1701Calcium 3 By similarity

Experimental info

Sequence conflict428 – 4303IAA → S in AAA33894. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
Q0D9J1-1 [UniParc].

Last modified October 17, 2006. Version 1.
Checksum: D7D61EC6DB04445E

FASTA44548,696
        10         20         30         40         50         60 
MATGRRLSMI LLLLLLGLAS GDKILFQGFN WESWRQSGGW YNLLMGKVDD IVAAGVTHVW 

        70         80         90        100        110        120 
LPPPSHSVST QGYMPGRLYD LDASRYGTSM ELKSLISALH GKGIQAIADV VINHRCADYK 

       130        140        150        160        170        180 
DSRGIYCIFE GGTPDGRLDW GPHMICRDDT QFSDGTGNLD TGADFAAAPD IDHLNGVVQR 

       190        200        210        220        230        240 
ELTDWLLWLK SDEVGFDAWR LDFARGYSPE VAKVYIEGTT PVGLAVAELW DSMAYGGDGK 

       250        260        270        280        290        300 
PEYNQDAHRQ ALVDWVDRVG GTASAGMVFD FTTKGIMNTA VEGELWRLID QQGKAPGVIG 

       310        320        330        340        350        360 
WWPAKAVTFV DNHDTGSTQQ MWPFPSDKVM QGYAYILTHP GNPCIFYDHF FDWGLKEQIA 

       370        380        390        400        410        420 
ALVAVRQRNG VTATSSLKIM LHDADAYVAE IDGKVVMKIG SRYDVSSLIP PGFHLAAHGN 

       430        440 
GYAVWEKIAA AAAAADHRTS SSASL

« Hide

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References

« Hide 'large scale' references
[1]"RAmy2A; a novel alpha-amylase-encoding gene in rice."
Huang N., Reinl S.J., Rodriguez R.L.
Gene 111:223-228(1992) [PubMed: 1541400] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. M202.
[2]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed: 16100779] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana."
The rice annotation project (RAP)
Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[4]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed: 12869764] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Nipponbare.

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Cross-references

Sequence databases

M74177 Genomic DNA. Translation: AAA33894.1.
AP004678 Genomic DNA. Translation: BAD54103.1.
AP008212 Genomic DNA. Translation: BAF20482.1.
AK101018 mRNA. No translation available.
PIRJQ1527.
RefSeqNP_001058568.1.
UniGeneOs.50953

3D structure databases

HSSPHSSP built from PDB template 1AVA based on UniProtKB P04063.
SMRQ0D9J1. Positions 23-427.
ModBaseSearch...

Protein family/group databases

CAZyGH13. Glycoside Hydrolase Family 13.

Genome annotation databases

GeneID4342055.
GenomeReviewsGene locus AMY2A in contig AP008212_GR.
KEGGosa:4342055.

Organism-specific databases

GrameneP27935.

Family and domain databases

InterProIPR012850. A-amylase_bs_C.
IPR013775. A-amylase_pln.
IPR006046. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat.
IPR006589. Glyco_hydro_13_sub_cat.
IPR013781. Glyco_hydro_sg_catalytic.
[Graphical view]
Gene3DG3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PfamPF07821. Alpha-amyl_C2. 1 hit.
PF00128. Alpha-amylase. 1 hit.
[Graphical view]
PIRSFPIRSF001028. Alph-amls_plant. 1 hit.
PRINTSPR00110. ALPHAAMYLASE.
SMARTSM00642. Aamy. 1 hit.
SM00810. Alpha-amyl_C2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameAMY2A_ORYSJ
AccessionPrimary (citable) accession number: Q0D9J1
Secondary accession number(s): P27935, P27941, Q5Z7T8
Entry history
Integrated into UniProtKB/Swiss-Prot: June 12, 2007
Last sequence update: October 17, 2006
Last modified: September 22, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents