Q0D076 (MNS1B_ASPTN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B
EC=3.2.1.113

Alternative name(s):
Class I alpha-mannosidase 1B
Man(9)-alpha-mannosidase 1B

Gene names

Name:	mns1B
Synonyms:	msdS
ORF Names:	ATEG_00658

Organism

Aspergillus terreus (strain NIH 2624 / FGSC A1156) [Complete proteome]

Taxonomic identifier

341663 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus › Aspergillus terreus

Protein attributes

Sequence length	508 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the maturation of Asn-linked oligosaccharides. Progressively trims alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2 By similarity.
Catalytic activity	Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.
Cofactor	Ca2+. Can also use Mg2+, but with lower efficiency By similarity.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasmic vesicle lumen By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 47 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasmic vesicle
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	protein glycosylation Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasmic membrane-bounded vesicle lumen Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: InterPro
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 508	487	Probable mannosyl-oligosaccharide alpha-1,2-mannosidase 1B		PRO_0000394822
Sites
	Active site	374	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	94	1	N-linked (GlcNAc...) Potential
	Glycosylation	114	1	N-linked (GlcNAc...) Potential
	Glycosylation	365	1	N-linked (GlcNAc...) Potential
	Disulfide bond	331 ↔ 360		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0D076 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 379FCDF6BCE2EE5B	FASTA	508	56,289
	10 20 30 40 50 60 MHLPSLSLPL AALTLATPSL AYPYYRTEQP LQNNLAQSRA NTIKEAFSHA WDGYKKYAYP 70 80 90 100 110 120 HDELHPVSNG YGDSRNGWGA SAVDALSTAI IMRNATIVNQ ILDHIAQVDY SKTNDTVSLF 130 140 150 160 170 180 ETTIRYLGGM LSGYDLLKGP ASGLVRDSKK VDMLLTQSKK LADVLKFAFD TPSGVPYNNI 190 200 210 220 230 240 NINTHGNDGA TSNGLAVTGT LVLEWTRLSD LTGDDEYAKL SQKAQSYLLK PQPASSEPFP 250 260 270 280 290 300 GLVGSHISIE TGEFTDAQVS WNGGDDSFYE YLIKMYVYDP EAFGEYKDRW EAAAQSSMEH 310 320 330 340 350 360 LASHPSTRPD LTFLASYNDG KLGLSSQHLT CFDGGSFLLG GTVLDNDDYI KFGLDLVKGC 370 380 390 400 410 420 HETYNATLTG IGPESFAWDP NSVPSNQKAL YEKAGFYIQS GAYILRPEVI ESFYYAYRIT 430 440 450 460 470 480 GEEQYREWIW NAFVAINKYC RTSSGFAGLQ DVNAANGGGR YDNQESFLFA EVMKYVYLAH 490 500 SGDAEWQVQR GNGNKFVFNT EAHPFRVR

References

[1]

"Annotation of the Aspergillus terreus NIH2624 genome." Birren B.W., Lander E.S., Galagan J.E., Nusbaum C., Devon K., Henn M., Ma L.-J., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M., Kleber M., Mauceli E.W., Brockman W., Rounsley S., Young S.K., LaButti K. , Pushparaj V., DeCaprio D., Crawford M., Koehrsen M., Engels R., Montgomery P., Pearson M., Howarth C., Larson L., Luoma S., White J., Alvarado L., Kodira C.D., Zeng Q., Oleary S., Yandava C., Denning D.W., Nierman W.C., Milne T., Madden K. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NIH 2624 / FGSC A1156.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH476594 Genomic DNA. Translation: EAU39304.1.
RefSeq	XP_001210744.1. XM_001210744.1.
3D structure databases
ProteinModelPortal	Q0D076.
SMR	Q0D076. Positions 37-507.
ModBase	Search...
Protein-protein interaction databases
STRING	33178.CADATEAP00009571.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADATEAT00009571; CADATEAP00009571; CADATEAG00009571.
GeneID	4355413.
Phylogenomic databases
eggNOG	NOG300315.
HOGENOM	HOG000181987.
OrthoDB	EOG42JS14.
Enzyme and pathway databases
UniPathway	UPA00378.
Family and domain databases
Gene3D	1.50.10.50. 1 hit.
InterPro	IPR001382. Glyco_hydro_47. [Graphical view]
PANTHER	PTHR11742. PTHR11742. 1 hit.
Pfam	PF01532. Glyco_hydro_47. 1 hit. [Graphical view]
PRINTS	PR00747. GLYHDRLASE47.
SUPFAM	SSF48225. Glyco_hydro_47. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MNS1B_ASPTN

Accession

Primary (citable) accession number: Q0D076

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 15, 2010
Last sequence update:	October 17, 2006
Last modified:	May 1, 2013
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program