ID   NCB5R_ASPTN             Reviewed;         296 AA.
AC   Q0CY37;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   16-JUN-2009, entry version 21.
DE   RecName: Full=NADH-cytochrome b5 reductase 1;
DE            EC=1.6.2.2;
DE   AltName: Full=Microsomal cytochrome b reductase;
GN   Name=cbr1; ORFNames=ATEG_01397;
OS   Aspergillus terreus (strain NIH 2624 / FGSC A1156).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Trichocomaceae;
OC   mitosporic Trichocomaceae; Aspergillus.
OX   NCBI_TaxID=341663;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Birren B.W., Lander E.S., Galagan J.E., Nusbaum C., Devon K., Henn M.,
RA   Ma L.-J., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M.,
RA   Kleber M., Mauceli E.W., Brockman W., Rounsley S., Young S.K.,
RA   LaButti K., Pushparaj V., DeCaprio D., Crawford M., Koehrsen M.,
RA   Engels R., Montgomery P., Pearson M., Howarth C., Larson L., Luoma S.,
RA   White J., Alvarado L., Kodira C.D., Zeng Q., Oleary S., Yandava C.,
RA   Denning D.W., Nierman W.C., Milne T., Madden K.;
RT   "Annotation of the Aspergillus terreus NIH2624 genome.";
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Electron donor reductase for cytochrome b5. The
CC       cytochrome b5/NADH cytochrome b5 reductase electron transfer
CC       system supports the catalytic activity of several sterol
CC       biosynthetic enzymes (By similarity).
CC   -!- CATALYTIC ACTIVITY: NADH + 2 ferricytochrome b5 = NAD(+) + H(+) +
CC       2 ferrocytochrome b5.
CC   -!- COFACTOR: FAD (By similarity).
CC   -!- SUBUNIT: Monomer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass
CC       membrane protein (By similarity). Mitochondrion outer membrane;
CC       Single-pass membrane protein (By similarity).
CC   -!- SIMILARITY: Belongs to the flavoprotein pyridine nucleotide
CC       cytochrome reductase family.
CC   -!- SIMILARITY: Contains 1 FAD-binding FR-type domain.
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DR   EMBL; CH476595; EAU38154.1; -; Genomic_DNA.
DR   RefSeq; XP_001208762.1; -.
DR   GeneID; 4315740; -.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004128; F:cytochrome-b5 reductase activity; IEA:EC.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR017927; Fd_Rdtase_FAD-bd.
DR   InterPro; IPR001709; Flavoprot_Pyr_Nucl_cyt_Rdtase.
DR   InterPro; IPR001834; NADH-Cyt_B5_reductase.
DR   InterPro; IPR008333; OxRdtase_FAD-bd.
DR   InterPro; IPR001433; OxRdtase_FAD/NAD_bd.
DR   Pfam; PF00970; FAD_binding_6; 1.
DR   Pfam; PF00175; NAD_binding_1; 1.
DR   PRINTS; PR00406; CYTB5RDTASE.
DR   PRINTS; PR00371; FPNCR.
DR   PROSITE; PS51384; FAD_FR; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Endoplasmic reticulum; FAD; Flavoprotein; Membrane;
KW   Mitochondrion; Mitochondrion outer membrane; NAD; Oxidoreductase;
KW   Transmembrane.
FT   CHAIN         1    296       NADH-cytochrome b5 reductase 1.
FT                                /FTId=PRO_0000330147.
FT   TRANSMEM     11     31       Potential.
FT   DOMAIN       47    152       FAD-binding FR-type.
FT   NP_BIND     132    147       FAD (By similarity).
FT   NP_BIND     158    195       FAD (By similarity).
SQ   SEQUENCE   296 AA;  32523 MW;  6E6CAF6589D35A17 CRC64;
     MSTFLQDNGD LSAVLVKFAP FAVAVIAILA AWKFTGSSKP RKVLNPSEFQ NFVLKEKTDI
     SHNVAIYRFA LPRPTDILGL PIGQHISLAA TIEGQPKEVV RSYTPISSDN EAGYFDLLVK
     AYPQGNISKY LTTLKIGDTL KVRGPKGAMV YTPNMCRHIG MIAGGTGITP MLQIIKAIIR
     NRPRNGGNDT TKIDLIFANV NEEDILLRDE LEKLAKEDDG FRIFYVLNNP PPGWNGGFGF
     VTAEMIKEHL PAPAKDVKIL LCGPPPMVSA MKKATESLGY TKARPVSKLE DQVFCF
//