Q0CXU6 (ATG15_ASPTN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative lipase atg15
EC=3.1.1.3

Alternative name(s):
Autophagy-related protein 15

Gene names

Name:	atg15
ORF Names:	ATEG_01488

Organism

Aspergillus terreus (strain NIH 2624 / FGSC A1156) [Complete proteome]

Taxonomic identifier

341663 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	613 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May be involved in lysis of subvacuolar cytoplasm to vacuole targeted bodies, intravacuolar autophagic bodies and of intravacuolar multivesicular body (MVB) vesicles By similarity.
Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity. Golgi apparatus membrane; Single-pass type II membrane protein By similarity. Endosome › multivesicular body membrane; Single-pass type II membrane protein By similarity. Prevacuolar compartment membrane; Single-pass type II membrane protein By similarity. Note: From ER, targeted to vacuolar lumen at the MVB vesicles via the Golgi and the prevacuolar compartment (PVC) By similarity.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family.

Ontologies

Keywords
Biological process	Autophagy Lipid degradation
Cellular component	Endoplasmic reticulum Endosome Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW multivesicular body membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triglyceride lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 613	613	Putative lipase atg15		PRO_0000317959
Regions
	Topological domain	1 – 20	20	Cytoplasmic By similarity
	Transmembrane	21 – 43	23	Helical; Signal-anchor for type II membrane protein
	Topological domain	44 – 613	570	Lumenal By similarity
	Compositional bias	473 – 587	115	Thr-rich
Sites
	Active site	322	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	202	1	N-linked (GlcNAc...) Potential
	Glycosylation	224	1	N-linked (GlcNAc...) Potential
	Glycosylation	282	1	N-linked (GlcNAc...) Potential
	Glycosylation	306	1	N-linked (GlcNAc...) Potential
	Glycosylation	468	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q0CXU6 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 199F93FF8C2DE22D	FASTA	613	66,953
	10 20 30 40 50 60 MKSHQGHQKK RRMREMGFST LLLGASLLLP VSVSASAPPS IYSRPYDSSP FLSPQIPLED 70 80 90 100 110 120 LSSLSGTHEF TLRHIFHRGT YQHPDLHRRL DIQPHTQLWV LSEDGTDKEA AEFDTPLIAS 130 140 150 160 170 180 SHPVTIQRLA DRRPSVIEEH LLAARMSGSA SVLSESDWVM DTLAGPDVTD KNSVLTFAKM 190 200 210 220 230 240 TANDYIQEPD TEDWQDIHGR FNYSSSFGWQ TDGLRGHIYA DKTNSTIVIS LKGTSPALFD 250 260 270 280 290 300 GAGTTTNDKL NDNLYFSCCC GQGGSYLWRQ VCDCQKSAFT ANLTCIIEAM NDENRYYRAA 310 320 330 340 350 360 LDLYSNVTEL YPDANVWLTG HSLGGAMTSL LGLTYGLPAV TFEAVPEALP AARLGLPSPP 370 380 390 400 410 420 GHDPRLPQSR RYTGAYHFGH TADPVYMGTC NGVSSVCTWG GYAMESACHT GQMCVYDTVD 430 440 450 460 470 480 DKGWRVAIGT HRIKAVISDV LEVYDDVPQC APEEECYDCE LWKFFRSNGS EHTTTTTTTT 490 500 510 520 530 540 TTTTTPPSTS TSTCKTPGWW GCLDESTTTT ATSTSTTSTS TTTCKTPGWF GCKDPTTSTT 550 560 570 580 590 600 ATPEPSLTTT LPLMTSTTCA HPGWFGCRDP TSPTSTPTQT PPPGDTTSCE SPGFFWGCWD 610 PKTTSDHPIT TPP

References

[1]

"Annotation of the Aspergillus terreus NIH2624 genome." Birren B.W., Lander E.S., Galagan J.E., Nusbaum C., Devon K., Henn M., Ma L.-J., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M., Kleber M., Mauceli E.W., Brockman W., Rounsley S., Young S.K., LaButti K. , Pushparaj V., DeCaprio D., Crawford M., Koehrsen M., Engels R., Montgomery P., Pearson M., Howarth C., Larson L., Luoma S., White J., Alvarado L., Kodira C.D., Zeng Q., Oleary S., Yandava C., Denning D.W., Nierman W.C., Milne T., Madden K. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NIH 2624 / FGSC A1156.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH476595 Genomic DNA. Translation: EAU38245.1.
RefSeq	XP_001208853.1. XM_001208853.1.
3D structure databases
ProteinModelPortal	Q0CXU6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADATEAT00001390; CADATEAP00001390; CADATEAG00001390.
GeneID	4315897.
Phylogenomic databases
GeneTree	EFGT00050000002657.
OrthoDB	EOG4F7RV2.
Family and domain databases
InterPro	IPR002921. Lipase_3. [Graphical view]
Pfam	PF01764. Lipase_3. 1 hit. [Graphical view]
PROSITE	PS00120. LIPASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG15_ASPTN

Accession

Primary (citable) accession number: Q0CXU6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 17, 2006
Last modified:	March 8, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program