Q0CUG5 (MANA2_ASPTN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable mannan endo-1,4-beta-mannosidase A-2
EC=3.2.1.78

Alternative name(s):
Endo-beta-1,4-mannanase A-2

Gene names

Name:	manA-2
Synonyms:	man1-2
ORF Names:	ATEG_02669

Organism

Aspergillus terreus (strain NIH 2624 / FGSC A1156) [Complete proteome]

Taxonomic identifier

341663 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus › Aspergillus terreus

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endo-1,4-mannanase, a crucial enzyme for depolymerization of seed galactomannans and wood galactoglucomannans By similarity.
Catalytic activity	Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family.
Sequence caution	The sequence EAU37631.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	mannan endo-1,4-beta-mannosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 396	375	Probable mannan endo-1,4-beta-mannosidase A-2		PRO_0000393704
Sites
	Active site	213	1	Proton donor By similarity
	Active site	321	1	Nucleophile By similarity
Amino acid modifications
	Glycosylation	120	1	N-linked (GlcNAc...) Potential
	Glycosylation	270	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q0CUG5 [UniParc]. Last modified April 20, 2010. Version 2. Checksum: D326B27A4DADC65C	FASTA	396	43,756
	10 20 30 40 50 60 MKVPRLLLAL GGLASIHIAS ALPQGAVERD MHATSAVVHG GNVKTKFPST TGLKFTIDGE 70 80 90 100 110 120 TGYFAGSNSY WIGFLTNNAD VDLVFQHMKE SGLKILRVWG FNDVNTKPAT GTVWYQLHAN 130 140 150 160 170 180 GTSTINTGPD GLQRLDYVVH SAERHGIKLI INFVNNWNDY GGINSYLQAY GGASNEDFYN 190 200 210 220 230 240 SEPMQKAYRA YIKEVVSRYI DSPAVFAWEL ANEPRCKGCD TSVLHSWIEK TSRYIKSLDK 250 260 270 280 290 300 KHMVTTGEEG FGLDLMSDGS YPFTYVEGGN FTDTLSIPTI DFGTFHLYPS SWGTTNDWGN 310 320 330 340 350 360 LWVEAHGAAC KAAGKPRLFE EYGVTADHCA LEKPWQTTAL KTKGVSGDLY WQYGDTLSTG 370 380 390 PSPDDGNTFY YGTDEFDCLV SDHVAAIDEM EKHGRH

References

[1]

"Annotation of the Aspergillus terreus NIH2624 genome." Birren B.W., Lander E.S., Galagan J.E., Nusbaum C., Devon K., Henn M., Ma L.-J., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M., Kleber M., Mauceli E.W., Brockman W., Rounsley S., Young S.K., LaButti K. , Pushparaj V., DeCaprio D., Crawford M., Koehrsen M., Engels R., Montgomery P., Pearson M., Howarth C., Larson L., Luoma S., White J., Alvarado L., Kodira C.D., Zeng Q., Oleary S., Yandava C., Denning D.W., Nierman W.C., Milne T., Madden K. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NIH 2624 / FGSC A1156.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH476596 Genomic DNA. Translation: EAU37631.1. Different initiation.
RefSeq	XP_001211847.1. XM_001211847.1.
3D structure databases
ProteinModelPortal	Q0CUG5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADATEAT00005204; CADATEAP00005204; CADATEAG00005204.
GeneID	4317245.
Phylogenomic databases
eggNOG	COG3934.
HOGENOM	HOG000169951.
OrthoDB	EOG44BF9Z.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR001547. Glyco_hydro_5. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00150. Cellulase. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00659. GLYCOSYL_HYDROL_F5. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MANA2_ASPTN

Accession

Primary (citable) accession number: Q0CUG5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 20, 2010
Last sequence update:	April 20, 2010
Last modified:	May 1, 2013
This is version 36 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program