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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trm8

Organism
Aspergillus terreus (strain NIH 2624 / FGSC A1156)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei101 – 1011S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei224 – 2241S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Active sitei227 – 2271UniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine, tRNA-binding

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
Transfer RNA methyltransferase 8UniRule annotation
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trm8
ORF Names:ATEG_03113
OrganismiAspergillus terreus (strain NIH 2624 / FGSC A1156)
Taxonomic identifieri341663 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000007963 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:ATEG_03113.

Subcellular locationi

  • Nucleus UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 346346tRNA (guanine-N(7)-)-methyltransferasePRO_0000370589Add
BLAST

Interactioni

Subunit structurei

Forms a complex with trm82.UniRule annotation

Protein-protein interaction databases

STRINGi33178.CADATEAP00008291.

Structurei

3D structure databases

ProteinModelPortaliQ0CT71.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni124 – 1252S-adenosyl-L-methionine bindingUniRule annotation
Regioni204 – 2052S-adenosyl-L-methionine bindingUniRule annotation
Regioni318 – 3203S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000260965.
OMAiRIENSTF.
OrthoDBiEOG708W9T.

Family and domain databases

Gene3Di3.40.50.150. 3 hits.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
InterProiIPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 2 hits.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0CT71-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNSRNKRGK REANRQRTVE ALARAQSGAD TGAALQLPQK KFYRQRAHAN
60 70 80 90 100
PFSDHQLKYP LSPAHMDWAS HYPAFENPDP AQTNLGGFRK LLKDVEVVDI
110 120 130 140 150
GCGFGGLLIG LAPLLPETLI VGMEIRIQVL DYVNTRIQAL RTQQRHFKLK
160 170 180 190 200
SAGGGGSDAA PESPAAPPTP SEAASPDSTT PSEQQAPTTL VPGGFQNISA
210 220 230 240 250
IRSNTMKFFP NFFGRGQLSK IFICFPDPHF KARKHKMRII SETLNAEYAY
260 270 280 290 300
ALRPGGLLYT ITDVEEYHHW ILRHFREPEV DGAVPCDDGA DGIKELFERV
310 320 330 340
SDEELQQDPC VEVMREATEE GKKVSRNKGN KYVAVFRRKA DPEWPA
Length:346
Mass (Da):38,508
Last modified:October 17, 2006 - v1
Checksum:iC25E452C8D22DCBE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH476597 Genomic DNA. Translation: EAU36387.1.
RefSeqiXP_001212291.1. XM_001212291.1.

Genome annotation databases

EnsemblFungiiCADATEAT00008291; CADATEAP00008291; CADATEAG00008291.
GeneIDi4318068.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH476597 Genomic DNA. Translation: EAU36387.1.
RefSeqiXP_001212291.1. XM_001212291.1.

3D structure databases

ProteinModelPortaliQ0CT71.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi33178.CADATEAP00008291.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADATEAT00008291; CADATEAP00008291; CADATEAG00008291.
GeneIDi4318068.

Organism-specific databases

EuPathDBiFungiDB:ATEG_03113.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000260965.
OMAiRIENSTF.
OrthoDBiEOG708W9T.

Enzyme and pathway databases

UniPathwayiUPA00989.

Family and domain databases

Gene3Di3.40.50.150. 3 hits.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
InterProiIPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 2 hits.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NIH 2624 / FGSC A1156.

Entry informationi

Entry nameiTRMB_ASPTN
AccessioniPrimary (citable) accession number: Q0CT71
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: October 17, 2006
Last modified: May 27, 2015
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.