Q0CT71 (TRMB_ASPTN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33

Alternative name(s):
Transfer RNA methyltransferase 8
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase

Gene names

Name:	trm8
ORF Names:	ATEG_03113

Organism

Aspergillus terreus (strain NIH 2624 / FGSC A1156) [Complete proteome]

Taxonomic identifier

341663 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus › Aspergillus terreus

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA.
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
Subunit structure	Forms a complex with trm82 By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Nucleus
Ligand	RNA-binding S-adenosyl-L-methionine tRNA-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	RNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	tRNA (guanine-N(7)-)-methyltransferase		PRO_0000370589
Regions
	Region	124 – 125	2	S-adenosyl-L-methionine binding By similarity
	Region	204 – 205	2	S-adenosyl-L-methionine binding By similarity
	Region	318 – 320	3	S-adenosyl-L-methionine binding By similarity
Sites
	Active site	227	1	By similarity
	Binding site	101	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	224	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0CT71 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: C25E452C8D22DCBE	FASTA	346	38,508
	10 20 30 40 50 60 MGNSRNKRGK REANRQRTVE ALARAQSGAD TGAALQLPQK KFYRQRAHAN PFSDHQLKYP 70 80 90 100 110 120 LSPAHMDWAS HYPAFENPDP AQTNLGGFRK LLKDVEVVDI GCGFGGLLIG LAPLLPETLI 130 140 150 160 170 180 VGMEIRIQVL DYVNTRIQAL RTQQRHFKLK SAGGGGSDAA PESPAAPPTP SEAASPDSTT 190 200 210 220 230 240 PSEQQAPTTL VPGGFQNISA IRSNTMKFFP NFFGRGQLSK IFICFPDPHF KARKHKMRII 250 260 270 280 290 300 SETLNAEYAY ALRPGGLLYT ITDVEEYHHW ILRHFREPEV DGAVPCDDGA DGIKELFERV 310 320 330 340 SDEELQQDPC VEVMREATEE GKKVSRNKGN KYVAVFRRKA DPEWPA

References

[1]

"Annotation of the Aspergillus terreus NIH2624 genome." Birren B.W., Lander E.S., Galagan J.E., Nusbaum C., Devon K., Henn M., Ma L.-J., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M., Kleber M., Mauceli E.W., Brockman W., Rounsley S., Young S.K., LaButti K. , Pushparaj V., DeCaprio D., Crawford M., Koehrsen M., Engels R., Montgomery P., Pearson M., Howarth C., Larson L., Luoma S., White J., Alvarado L., Kodira C.D., Zeng Q., Oleary S., Yandava C., Denning D.W., Nierman W.C., Milne T., Madden K. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NIH 2624 / FGSC A1156.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH476597 Genomic DNA. Translation: EAU36387.1.
RefSeq	XP_001212291.1. XM_001212291.1.
3D structure databases
ProteinModelPortal	Q0CT71.
ModBase	Search...
Protein-protein interaction databases
STRING	33178.CADATEAP00008291.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADATEAT00008291; CADATEAP00008291; CADATEAG00008291.
GeneID	4318068.
Phylogenomic databases
eggNOG	COG0220.
HOGENOM	HOG000260965.
OrthoDB	EOG4M3DJF.
Enzyme and pathway databases
UniPathway	UPA00989.
Family and domain databases
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. IPR025763. tRNA_(Gua-N-7)_MeTrfase_euk. [Graphical view]
Pfam	PF02390. Methyltransf_4. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRMB_ASPTN

Accession

Primary (citable) accession number: Q0CT71

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	October 17, 2006
Last modified:	April 3, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program