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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trm8

Organism
Aspergillus terreus (strain NIH 2624 / FGSC A1156)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei101S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei224S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei227UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase, tRNA-binding
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
Transfer RNA methyltransferase 8UniRule annotation
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trm8
ORF Names:ATEG_03113
OrganismiAspergillus terreus (strain NIH 2624 / FGSC A1156)
Taxonomic identifieri341663 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000007963 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:ATEG_03113

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003705891 – 346tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST346

Proteomic databases

PRIDEiQ0CT71

Interactioni

Subunit structurei

Forms a complex with trm82.UniRule annotation

Protein-protein interaction databases

STRINGi33178.CADATEAP00008291

Structurei

3D structure databases

ProteinModelPortaliQ0CT71
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni124 – 125S-adenosyl-L-methionine bindingUniRule annotation2
Regioni204 – 205S-adenosyl-L-methionine bindingUniRule annotation2
Regioni318 – 320S-adenosyl-L-methionine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000260965
OMAiIGMEIRV
OrthoDBiEOG092C3FCG

Family and domain databases

HAMAPiMF_03055 tRNA_methyltr_TrmB_euk, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR025763 Trm8_euk
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
PTHR23417:SF16 PTHR23417:SF16, 1 hit
SUPFAMiSSF53335 SSF53335, 2 hits
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

Q0CT71-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNSRNKRGK REANRQRTVE ALARAQSGAD TGAALQLPQK KFYRQRAHAN
60 70 80 90 100
PFSDHQLKYP LSPAHMDWAS HYPAFENPDP AQTNLGGFRK LLKDVEVVDI
110 120 130 140 150
GCGFGGLLIG LAPLLPETLI VGMEIRIQVL DYVNTRIQAL RTQQRHFKLK
160 170 180 190 200
SAGGGGSDAA PESPAAPPTP SEAASPDSTT PSEQQAPTTL VPGGFQNISA
210 220 230 240 250
IRSNTMKFFP NFFGRGQLSK IFICFPDPHF KARKHKMRII SETLNAEYAY
260 270 280 290 300
ALRPGGLLYT ITDVEEYHHW ILRHFREPEV DGAVPCDDGA DGIKELFERV
310 320 330 340
SDEELQQDPC VEVMREATEE GKKVSRNKGN KYVAVFRRKA DPEWPA
Length:346
Mass (Da):38,508
Last modified:October 17, 2006 - v1
Checksum:iC25E452C8D22DCBE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH476597 Genomic DNA Translation: EAU36387.1
RefSeqiXP_001212291.1, XM_001212291.1

Genome annotation databases

EnsemblFungiiCADATEAT00008291; CADATEAP00008291; CADATEAG00008291
GeneIDi4318068

Similar proteinsi

Entry informationi

Entry nameiTRMB_ASPTN
AccessioniPrimary (citable) accession number: Q0CT71
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: October 17, 2006
Last modified: May 23, 2018
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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