Q0CRF7 (CBHA_ASPTN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable 1,4-beta-D-glucan cellobiohydrolase A
EC=3.2.1.91

Alternative name(s):
Beta-glucancellobiohydrolase A
Cellobiohydrolase D
Exocellobiohydrolase A
Exoglucanase A

Gene names

Name:	cbhA
Synonyms:	celD
ORF Names:	ATEG_03727

Organism

Aspergillus terreus (strain NIH 2624 / FGSC A1156) [Complete proteome]

Taxonomic identifier

341663 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus › Aspergillus terreus

Protein attributes

Sequence length	453 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the dissaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose By similarity.
Catalytic activity	Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.
Subcellular location	Secreted Probable.
Sequence similarities	Belongs to the glycosyl hydrolase 7 (cellulase C) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cellulose 1,4-beta-cellobiosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 453	436	Probable 1,4-beta-D-glucan cellobiohydrolase A		PRO_0000393543
Sites
	Active site	227	1	Nucleophile By similarity
	Active site	232	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	62	1	N-linked (GlcNAc...) Potential
	Glycosylation	285	1	N-linked (GlcNAc...) Potential
	Glycosylation	388	1	N-linked (GlcNAc...) Potential
	Glycosylation	450	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q0CRF7 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 759ABB134EDBBEFA	FASTA	453	48,073
	10 20 30 40 50 60 MHQRALLFSA LVGAVRAQQA GTLTEEVHPP LTWQKCTADG SCTEQSGSVV IDSNWRWLHS 70 80 90 100 110 120 TNGSTNCYTG NTWDESLCPD NEACAANCAL DGADYESTYG ITTSGDALTL TFVTGENVGS 130 140 150 160 170 180 RVYLMAEDDE SYQTFDLVGN EFTFDVDVSN LPCGLNGALY FTSMDADGGV SKYPANKAGA 190 200 210 220 230 240 KYGTGYCDSQ CPRDLKFING MANVEGWTPS DNDKNAGVGG HGSCCPELDI WEANSISSAF 250 260 270 280 290 300 TPHPCDDLGQ TMCSGDDCGG TYSETRYAGT CDPDGCDFNA YRMGNTSYYG PDKIVDTNSV 310 320 330 340 350 360 MTVVTQFIGD GGSLSEIKRL YVQNGKVIAN AQSNVDGVTG NSITSDFCTA QKTAFGDQDI 370 380 390 400 410 420 FSKHGGLSGM GDAMSAMVLI LSIWDDHNSS MMWLDSTYPE DADASEPGVA RGTCEHGVGD 430 440 450 PETVESQHPG ATVTFSKIKF GPIGSTYSSN STA

References

[1]

"Annotation of the Aspergillus terreus NIH2624 genome." Birren B.W., Lander E.S., Galagan J.E., Nusbaum C., Devon K., Henn M., Ma L.-J., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M., Kleber M., Mauceli E.W., Brockman W., Rounsley S., Young S.K., LaButti K. , Pushparaj V., DeCaprio D., Crawford M., Koehrsen M., Engels R., Montgomery P., Pearson M., Howarth C., Larson L., Luoma S., White J., Alvarado L., Kodira C.D., Zeng Q., Oleary S., Yandava C., Denning D.W., Nierman W.C., Milne T., Madden K. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NIH 2624 / FGSC A1156.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH476598 Genomic DNA. Translation: EAU35529.1.
RefSeq	XP_001212905.1. XM_001212905.1.
3D structure databases
ProteinModelPortal	Q0CRF7.
SMR	Q0CRF7. Positions 18-448.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADATEAT00005976; CADATEAP00005976; CADATEAG00005976.
GeneID	4318706.
Phylogenomic databases
eggNOG	NOG85664.
HOGENOM	HOG000182210.
OrthoDB	EOG4K3Q4P.
Family and domain databases
Gene3D	2.70.100.10. 1 hit.
InterPro	IPR008985. ConA-like_lec_gl_sf. IPR001722. Glyco_hydro_7. [Graphical view]
Pfam	PF00840. Glyco_hydro_7. 1 hit. [Graphical view]
PRINTS	PR00734. GLHYDRLASE7.
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CBHA_ASPTN

Accession

Primary (citable) accession number: Q0CRF7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 20, 2010
Last sequence update:	October 17, 2006
Last modified:	March 6, 2013
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program