Reviewed, UniProtKB/Swiss-Prot Q0CRD8 (MCR1_ASPTN)

Names and origin

Protein names

Recommended name:
    NADH-cytochrome b5 reductase 2
    EC=1.6.2.2
Alternative name(s):
    Mitochondrial cytochrome b reductase

Gene names

Name:	mcr1
ORF Names:	ATEG_03746

Organism

Aspergillus terreus (strain NIH 2624 / FGSC A1156) [Complete proteome]

Taxonomic identifier

341663 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May mediate the reduction of outer membrane cytochrome b5 By similarity.
Catalytic activity	NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.
Cofactor	FAD By similarity.
Subcellular location	Mitochondrion outer membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.
Sequence caution	The sequence EAU35548.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cytochrome-b5 reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	NADH-cytochrome b5 reductase 2		PRO_0000330174
Regions
	Transmembrane	30 – 46	17	Potential
	Domain	69 – 173	105	FAD-binding FR-type
	Nucleotide binding	176 – 211	36	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0CRD8-1 [UniParc]. Last modified April 29, 2008. Version 2. Checksum: F67DDE30D95B91A9	FASTA	319	35,834
	10 20 30 40 50 60 MFARQTFRYA QPLKQSFRKY STEAPKGKSL APVYLTVGLA GLGVGLYRYN SATAEAPAER 70 80 90 100 110 120 AKVFTGGDQG WVDLKLSEIE VLNHNTKRFR FEFEDKEAVS GLNVASALLT KFKPEGGKAV 130 140 150 160 170 180 LRPYTPVSDE SQPGFLDLVV KVYPNGPMSE HLHSMNVDQR LEFKGPLPKY PWEANKHQHI 190 200 210 220 230 240 CLIAGGTGIT PMYQLARHIF KNPEDKTKVT LVYGNVSEQD ILLKKELEEL ENTYPQRFKA 250 260 270 280 290 300 FYVLDNPPKE WTGGKGYISK ELLKTVLPEP KEENIKIFVC GPPGLYKAIS GNKVSPKDQG 310 ELTGILKELG YSQEQVFKF

References

[1]

"Annotation of the Aspergillus terreus NIH2624 genome." Birren B.W., Lander E.S., Galagan J.E., Nusbaum C., Devon K., Henn M., Ma L.-J., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M., Kleber M., Mauceli E.W., Brockman W., Rounsley S., Young S.K., LaButti K. , Pushparaj V., DeCaprio D., Crawford M., Koehrsen M., Engels R., Montgomery P., Pearson M., Howarth C., Larson L., Luoma S., White J., Alvarado L., Kodira C.D., Zeng Q., Oleary S., Yandava C., Denning D.W., Nierman W.C., Milne T., Madden K. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH476598 Genomic DNA. Translation: EAU35548.1. Sequence problems.
RefSeq	XP_001212924.1.
3D structure databases
SMR	Q0CRD8. Positions 69-319.
ModBase	Search...
Genome annotation databases
GeneID	4318618.
Phylogenomic databases
OrthoDB	EOG9TB5TC.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd_dom. IPR001433. OxRdtase_FAD/NAD_bd. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MCR1_ASPTN

Accession

Primary (citable) accession number: Q0CRD8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	April 29, 2008
Last modified:	January 19, 2010
This is version 23 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)