Q0CQK1 (SSN3_ASPTN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase ssn3
EC=2.7.11.22
EC=2.7.11.23

Alternative name(s):
Cyclin-dependent kinase 8

Gene names

Name:	ssn3
Synonyms:	cdk8
ORF Names:	ATEG_04033

Organism

Aspergillus terreus (strain NIH 2624 / FGSC A1156) [Complete proteome]

Taxonomic identifier

341663 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus › Aspergillus terreus

Protein attributes

Sequence length	435 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Component of the srb8-11 complex. The srb8-11 complex is a regulatory module of the Mediator complex which is itself involved in regulation of basal and activated RNA polymerase II-dependent transcription. The srb8-11 complex may be involved in the transcriptional repression of a subset of genes regulated by Mediator. It may inhibit the association of the Mediator complex with RNA polymerase II to form the holoenzyme complex. The srb8-11 complex phosphorylates the C-terminal domain (CTD) of the largest subunit of RNA polymerase II By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Cofactor	Magnesium By similarity.
Subunit structure	Component of the srb8-11 complex, a regulatory module of the Mediator complex By similarity.
Subcellular location	Nucleus Probable.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Activator Kinase Repressor Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: GOC regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 435	435	Serine/threonine-protein kinase ssn3		PRO_0000312937
Regions
	Domain	49 – 377	329	Protein kinase
	Nucleotide binding	55 – 63	9	ATP By similarity
Sites
	Active site	181	1	Proton acceptor By similarity
	Binding site	79	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0CQK1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 802A04756FBAE615	FASTA	435	49,087
	10 20 30 40 50 60 MYGRNFPFFN SLGGFYPRGN GFPLDAPSLD PRKPSGTGYT SKVRVRDKYR IVGFISSGTY 70 80 90 100 110 120 GRVYKAIGKN GEKREFAIKK FKPDKEGEII QYTGLSQSAI REMSLCSELD HPNVVQLEEI 130 140 150 160 170 180 ILEDKCIFMV FEYTEHDLLQ IIHHHTQPQR HAIPAPMVRS ILFQLLNGLL YLHTNWVLHR 190 200 210 220 230 240 DLKPANILVT SSGAIRVGDL GLARLFYKPL NSLFSGDKVV VTIWYRAPEL LMGSRHYTPA 250 260 270 280 290 300 VDLWAVGCIF AELLSLRPIF KGEEAKMDSK KTVPFQRNQM MKIIEIMGLP TKEIWPGITS 310 320 330 340 350 360 MPEFSQLQSL AMSRAPHFNR SSNLENWYQS CLKNGGYSAN SSAGTPGADG FDLLSRLLEY 370 380 390 400 410 420 DPTKRITARE ALEHPYFKNG GPISANCFEG FEGKYPHRRV TQDDNDIRSG SLPGTKRSGL 430 PDDSLMGRAS KRIKE

References

[1]

"Annotation of the Aspergillus terreus NIH2624 genome." Birren B.W., Lander E.S., Galagan J.E., Nusbaum C., Devon K., Henn M., Ma L.-J., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M., Kleber M., Mauceli E.W., Brockman W., Rounsley S., Young S.K., LaButti K. , Pushparaj V., DeCaprio D., Crawford M., Koehrsen M., Engels R., Montgomery P., Pearson M., Howarth C., Larson L., Luoma S., White J., Alvarado L., Kodira C.D., Zeng Q., Oleary S., Yandava C., Denning D.W., Nierman W.C., Milne T., Madden K. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NIH 2624 / FGSC A1156.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH476598 Genomic DNA. Translation: EAU35835.1.
RefSeq	XP_001213211.1. XM_001213211.1.
3D structure databases
ProteinModelPortal	Q0CQK1.
ModBase	Search...
Protein-protein interaction databases
STRING	33178.CADATEAP00009663.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADATEAT00009663; CADATEAP00009663; CADATEAG00009663.
GeneID	4318303.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000233024.
OrthoDB	EOG470XRW.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SSN3_ASPTN

Accession

Primary (citable) accession number: Q0CQK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 17, 2006
Last modified:	May 1, 2013
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program