Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable beta-galactosidase B

Gene

lacB

Organism
Aspergillus terreus (strain NIH 2624 / FGSC A1156)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.By similarity

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei90 – 901SubstrateBy similarity
Binding sitei135 – 1351SubstrateBy similarity
Binding sitei136 – 1361Substrate; via amide nitrogenBy similarity
Binding sitei137 – 1371SubstrateBy similarity
Binding sitei195 – 1951SubstrateBy similarity
Active sitei196 – 1961Proton donorSequence Analysis
Binding sitei265 – 2651SubstrateBy similarity
Active sitei308 – 3081NucleophileSequence Analysis
Binding sitei373 – 3731SubstrateBy similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Probable beta-galactosidase B (EC:3.2.1.23)
Alternative name(s):
Lactase B
Gene namesi
Name:lacB
ORF Names:ATEG_05131
OrganismiAspergillus terreus (strain NIH 2624 / FGSC A1156)
Taxonomic identifieri341663 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000007963: Unassembled WGS sequence

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 10221002Probable beta-galactosidase BPRO_0000395227Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi23 – 231N-linked (GlcNAc...)Sequence Analysis
Glycosylationi100 – 1001N-linked (GlcNAc...)Sequence Analysis
Glycosylationi211 – 2111N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi271 ↔ 324By similarity
Glycosylationi411 – 4111N-linked (GlcNAc...)Sequence Analysis
Glycosylationi456 – 4561N-linked (GlcNAc...)Sequence Analysis
Glycosylationi541 – 5411N-linked (GlcNAc...)Sequence Analysis
Glycosylationi554 – 5541N-linked (GlcNAc...)Sequence Analysis
Glycosylationi626 – 6261N-linked (GlcNAc...)Sequence Analysis
Glycosylationi777 – 7771N-linked (GlcNAc...)Sequence Analysis
Glycosylationi790 – 7901N-linked (GlcNAc...)Sequence Analysis
Glycosylationi832 – 8321N-linked (GlcNAc...)Sequence Analysis
Glycosylationi880 – 8801N-linked (GlcNAc...)Sequence Analysis
Glycosylationi881 – 8811N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi33178.CADATEAP00004534.

Structurei

3D structure databases

ProteinModelPortaliQ0CMF3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1874.
HOGENOMiHOG000181922.
OrthoDBiEOG7ZGXBD.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SMARTiSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0CMF3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARFPQLLFL LLASIGLLSA AQNHSDSEWP LHDNGLSTVV QWDHYSFHVH
60 70 80 90 100
GQRIFVFSGE FHYWRIPVPG LWRDILEKIK AAGFTAFAFY SSWGYHAPNN
110 120 130 140 150
HTVDFSTGAR DITPIYELAK ELGMYIIVRP GPYVNAEASA GGYPLWVTTG
160 170 180 190 200
AYGSLRNDDA RYTAAWKPYF AKMSEITSQY QVTDGHNTFC YQIENEYGQQ
210 220 230 240 250
WIGDPVDRNP NQTAVAYMEL LEASARENGI VVPLTANDPN MNTKSWGSDW
260 270 280 290 300
SHAGGNVDVV GLDSYPSCWT CDVTQCTSTN GEYVPYKVMQ YYDYFQEVQP
310 320 330 340 350
TMPGFMPEFQ GGSYNPWAGP EGGCPGDTGV DFANLFYRWN IAQRVTAMSL
360 370 380 390 400
YMLYGGTNWG AIAAPVTATS YDYSSPISED RSIGSKYYET KLLALFTRSA
410 420 430 440 450
TDLTMTDRIG NGTHYTNNPA VAAYELRNPV TNGAFYVTIH ADSTVGTDES
460 470 480 490 500
FRLNVNTSAG ALTVPSKGSI RLNGHQSKII VTDFRFGPSH TLLYSTAEVL
510 520 530 540 550
THAVMDKKAT LVLWVPTGES GEFAVKGAKS GKVERCPQCS NATFTRKKDV
560 570 580 590 600
LVVNFTQAGG MSVLQLNNGV RVVLLDRAAA YKFWAPPLTD DPFAPETDLV
610 620 630 640 650
LVQGPYLVRS ASLSGSTLAL RGDSANETAL EVFASKKVHT VTWNGKRIKT
660 670 680 690 700
SRSSYGSLTA SLAAPPAVSL PALSSAQWKS QDSLPERLPS YDDSGPAWVD
710 720 730 740 750
ANHMTTQNPR TPDTLPVLYA DEYGFHNGIR LWRGSFTDAA SGVYLNVQGG
760 770 780 790 800
AAFGWSAYLN GHFLGSHLGT ATTSQANKTL LFPAGTLRKN TTNTILVIHD
810 820 830 840 850
DTGHDQTTGA LNPRGILAAR LLAPSDSSTA PNFTQWRVAG TAGGESDLDP
860 870 880 890 900
VRGVYNEDGL FAERMGWHLP GFDDADWPAN NSTTTRGAQV SLSVTGATVR
910 920 930 940 950
FFRAVVPLHL PRGVDASISF MLGTPAGAST AYRAQLFVNG YQYGRFYPHI
960 970 980 990 1000
GNQVVYPVPA GVLDYDGENT IGVAVWAQSE AGAEMSLDWR VNYVADSSLD
1010 1020
AVRVAAEGAL RPGWSEERLQ YA
Length:1,022
Mass (Da):111,471
Last modified:July 13, 2010 - v2
Checksum:i032C051812784E46
GO

Sequence cautioni

The sequence EAU34200.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH476600 Genomic DNA. Translation: EAU34200.1. Sequence problems.
RefSeqiXP_001214309.1. XM_001214309.1.

Genome annotation databases

GeneIDi4320669.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH476600 Genomic DNA. Translation: EAU34200.1. Sequence problems.
RefSeqiXP_001214309.1. XM_001214309.1.

3D structure databases

ProteinModelPortaliQ0CMF3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi33178.CADATEAP00004534.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi4320669.

Phylogenomic databases

eggNOGiCOG1874.
HOGENOMiHOG000181922.
OrthoDBiEOG7ZGXBD.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SMARTiSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NIH 2624 / FGSC A1156.

Entry informationi

Entry nameiBGALB_ASPTN
AccessioniPrimary (citable) accession number: Q0CMF3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: July 13, 2010
Last modified: January 7, 2015
This is version 49 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.