Reviewed, UniProtKB/Swiss-Prot Q0CKU4 (ATG26_ASPTN)

Names and origin

Protein names

Recommended name:
    Sterol 3-beta-glucosyltransferase
    EC=2.4.1.173
Alternative name(s):
    Autophagy-related protein 26

Gene names

Name:	atg26
ORF Names:	ATEG_05690

Organism

Aspergillus terreus (strain NIH 2624 / FGSC A1156) [Complete proteome]

Taxonomic identifier

341663 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	1396 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May be involved in decane metabolism and autophagy. Involved in the biosynthesis of sterol glucoside By similarity.
Catalytic activity	UDP-glucose + a sterol = UDP + a sterol 3-beta-D-glucoside.
Subcellular location	Cytoplasm By similarity. Membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 28 family. Contains 2 GRAM domains. Contains 1 PH domain.

Ontologies

Keywords
Biological process	Autophagy Lipid synthesis Protein transport Steroid biosynthesis Sterol biosynthesis Transport
Cellular component	Cytoplasm Membrane
Domain	Repeat
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro lipid glycosylation Inferred from electronic annotation. Source: InterPro protein transport Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sterol 3-beta-glucosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1396	1396	Sterol 3-beta-glucosyltransferase		PRO_0000318043
Regions
	Domain	237 – 288	52	GRAM 1
	Domain	289 – 387	99	PH
	Domain	719 – 785	67	GRAM 2

Sequences

	Sequence		Length	Mass (Da)
	Q0CKU4-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 0F29E96658D0B63D	FASTA	1,396	156,512
	10 20 30 40 50 60 MRPLLDEAKR RVDRRLSASR QSLSTSRIFP SALPERLKDD HDAQVDYTAP PGGGGTKEGH 70 80 90 100 110 120 FQYMQQSIFG MIAAVGSQSD FHARFDDSSD SERDTDRPPQ KRTEKESQAT DAPPSSKNEK 130 140 150 160 170 180 KALRSPQRPK SPSQERGRRH RKTLSGSRIL RPLMPGSSQR QGGAQTEPST GNQMSRVPSP 190 200 210 220 230 240 ERPRSTTPRA APVLSRMVEA QALFDSKGSM NQSPRSPPAE TQEEQSQEQT SASPLSLRLM 250 260 270 280 290 300 EMFGFESPEK VLVEYACSLV QSMLLQGYMY VTEGHICFYA YLPRKSTVAI KSGYLYKRGR 310 320 330 340 350 360 KNPKYNRYWF SLKGDVLSYY ADPSNLYFPS GHIDLRYGIS ASLSEPKEKD REARDFQVTT 370 380 390 400 410 420 DQRTYYFRAD SSTSAKEWVK SLQKVIFRTH NDGDSVKISF PIESIIDIEE SPMVDFAETF 430 440 450 460 470 480 KIRAIESGET YAIDEYYFSF FNDGQDAFNF VKGLVSESQA KNPSRESPQP GRTTPQSRAR 490 500 510 520 530 540 GSRARWSLTS GLSQVLGSSD ARRRRSASAS QFSPGRDAAG LSPTSRQRDL SESFVNSFDQ 550 560 570 580 590 600 ATESSTVLQS MTDTAESASQ ILNRSDVFQY PAMQPFRRQS LSEDQFGRRH SDETARSTND 610 620 630 640 650 660 IARLGPGITP RDVQRFYPPS DSDHDAQDAA RVQQSASSLN ELVRAGAYPL QRAAGLAEYL 670 680 690 700 710 720 RNRSKQMGTL LASESMGYIE KVSGMWAGGR RHYGEAEGIL PDDQDVDPED KEDGCNHGDR 730 740 750 760 770 780 FRAHFALPPT EKLQATYFAY LHRVLPLYGK IYVSQKKLCF RSLLPGTRTK MILPLKDVEN 790 800 810 820 830 840 VEKEKGFRFG YHGLVVIIRG HEELFFEFNA ADVRDDCAVT IHQHLESVRF LSESGMLAEQ 850 860 870 880 890 900 EQDESEAAKA EHRMLQEARK DASGGLIPQT PSDESPEIHP IFDDPRASII NFKPTESLRI 910 920 930 940 950 960 TCLTIGSRGD VQPYIALCKG LLAEGHRPKI ATHAEFEPWI RKHGIDFAPV EGDPAELMRI 970 980 990 1000 1010 1020 CVENGMFTYS FLKEASMKFR GWIDDLLSSA WRSCQDSDLL IESPSAMAGI HIAEALRIPY 1030 1040 1050 1060 1070 1080 FRAFTMPWTR TRAYPHAFAV PEHKMGGAYN YITYVMFDNV FWKAIAGQVN RWRQSELGLK 1090 1100 1110 1120 1130 1140 ATNLDKMQPN KVPFLYNYSP SVVVPPLDYP DWIRITGYWF LSEASDWTPP ADLMAFIQRA 1150 1160 1170 1180 1190 1200 RDDGKKLVYI GFGSIVVSDP SALTRTVVES VQKADVRCIL SKGWSDRLGD PASVKSEIPL 1210 1220 1230 1240 1250 1260 PPEIFQIQAA PHDWLFSQID AAAHHGGAGT TGASLRAGVP TIVKPFFGDQ FFFGTRVEDL 1270 1280 1290 1300 1310 1320 GVGICLKRLN VSLFSRALWE ATHSERMIVK ARNLGQQIRS EDGVATAIQA IYRDLEYAKT 1330 1340 1350 1360 1370 1380 LARQRSIVSS TPFSPTPSAK TVAEQEVDDD VTDSEEWTFI GDETDIDISR RVRGRAVSDV 1390 DMLPEPLAVR SPELAQ

References

[1]

"Annotation of the Aspergillus terreus NIH2624 genome." Birren B.W., Lander E.S., Galagan J.E., Nusbaum C., Devon K., Henn M., Ma L.-J., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M., Kleber M., Mauceli E.W., Brockman W., Rounsley S., Young S.K., LaButti K. , Pushparaj V., DeCaprio D., Crawford M., Koehrsen M., Engels R., Montgomery P., Pearson M., Howarth C., Larson L., Luoma S., White J., Alvarado L., Kodira C.D., Zeng Q., Oleary S., Yandava C., Denning D.W., Nierman W.C., Milne T., Madden K. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH476601 Genomic DNA. Translation: EAU33451.1.
RefSeq	XP_001214868.1.
3D structure databases
SMR	Q0CKU4. Positions 290-386.
ModBase	Search...
Genome annotation databases
GeneID	4321815.
Phylogenomic databases
OrthoDB	EOG9908S8.
Family and domain databases
InterPro	IPR004276. Glyco_trans_28. IPR004182. GRAM. IPR011993. PH_type. IPR001849. Pleckstrin_homology. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
Pfam	PF03033. Glyco_transf_28. 1 hit. PF02893. GRAM. 2 hits. PF00169. PH. 1 hit. [Graphical view]
SMART	SM00568. GRAM. 2 hits. SM00233. PH. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG26_ASPTN

Accession

Primary (citable) accession number: Q0CKU4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 17, 2006
Last modified:	January 19, 2010
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)