ID   BTGC_ASPTN              Reviewed;         655 AA.
AC   Q0CI96;
DT   15-JUN-2010, integrated into UniProtKB/Swiss-Prot.
DT   15-JUN-2010, sequence version 2.
DT   22-FEB-2023, entry version 61.
DE   RecName: Full=Probable glucan endo-1,3-beta-glucosidase btgC;
DE            EC=3.2.1.39;
DE   AltName: Full=Endo-1,3-beta-glucanase btgC;
DE   AltName: Full=Laminarinase btgC;
GN   Name=btgC; ORFNames=ATEG_06588;
OS   Aspergillus terreus (strain NIH 2624 / FGSC A1156).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=341663;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NIH 2624 / FGSC A1156;
RA   Birren B.W., Lander E.S., Galagan J.E., Nusbaum C., Devon K., Henn M.,
RA   Ma L.-J., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M.,
RA   Kleber M., Mauceli E.W., Brockman W., Rounsley S., Young S.K., LaButti K.,
RA   Pushparaj V., DeCaprio D., Crawford M., Koehrsen M., Engels R.,
RA   Montgomery P., Pearson M., Howarth C., Larson L., Luoma S., White J.,
RA   Alvarado L., Kodira C.D., Zeng Q., Oleary S., Yandava C., Denning D.W.,
RA   Nierman W.C., Milne T., Madden K.;
RT   "Annotation of the Aspergillus terreus NIH2624 genome.";
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Glucanases play a role in cell expansion during growth, in
CC       cell-cell fusion during mating, and in spore release during
CC       sporulation. This enzyme may be involved in beta-glucan degradation.
CC       Active on laminarin and lichenan (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-
CC         beta-D-glucans.; EC=3.2.1.39;
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Single-pass type II
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 17 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=EAU33132.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=EAU33132.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; CH476602; EAU33132.1; ALT_SEQ; Genomic_DNA.
DR   RefSeq; XP_001215766.1; XM_001215766.1.
DR   AlphaFoldDB; Q0CI96; -.
DR   SMR; Q0CI96; -.
DR   STRING; 341663.Q0CI96; -.
DR   GlyCosmos; Q0CI96; 3 sites, No reported glycans.
DR   EnsemblFungi; EAU33132; EAU33132; ATEG_06588.
DR   GeneID; 4322381; -.
DR   eggNOG; ENOG502QTKT; Eukaryota.
DR   OrthoDB; 675117at2759; -.
DR   Proteomes; UP000007963; Unassembled WGS sequence.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0042973; F:glucan endo-1,3-beta-D-glucosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0000272; P:polysaccharide catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR16631; GLUCAN 1,3-BETA-GLUCOSIDASE; 1.
DR   PANTHER; PTHR16631:SF17; GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BTGC; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism; Cell membrane; Cell wall biogenesis/degradation;
KW   Glycoprotein; Hydrolase; Membrane; Polysaccharide degradation;
KW   Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix.
FT   CHAIN           1..655
FT                   /note="Probable glucan endo-1,3-beta-glucosidase btgC"
FT                   /id="PRO_0000395127"
FT   TOPO_DOM        1..282
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        283..303
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        304..655
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   REGION          1..61
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          89..114
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          305..338
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..36
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        310..324
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        458
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:O22317"
FT   ACT_SITE        557
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:O22317"
FT   CARBOHYD        426
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        576
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        602
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   655 AA;  69420 MW;  C2560B77C3B3CAC5 CRC64;
     MSGDPRSFSF NQGDDHPIDS SQQPLHPTNT MADSYSDRNW GAPGGLDHTH SMRTQSTATP
     GMDNLGPAAV GGGISGIALG VANSHDRQSG VDAFRDTDGG NFPAERGYNA PGSDNPYVPP
     PPAAAYDSSD NLTARSGAYG SSAALAAAAS APAGASNTSR RSFVDSPYQG VGALDAGPYQ
     RQSVYNNGDY PLVINPDEII DDGDDGFALP NSKSAGHKSR AVPAAAAGAA GGAAAGGLLG
     GIFKSKAAAE GPSYGPVPGA GIEAAEKGQW AKPKPGTGSR KRGWIVGIIL AVVIVGAIVG
     GAVGGTLGNR EKESPSSSET ASGDEKVNGD LGKDSDEIKS LMNNPNLHKV FPGMDYTPWG
     TQYPLCQKYP PSQNNVTRDI AVLSQLTNTV RLYGTDCNQT EMVLHAIDRL ELTEMKLWLG
     VWIDTNKTTC ERQLNQLYDV LDKTKDHSIF KGAIIGNEAL YRAGSSIAEA EKTLISYMTE
     VRDHFKKNNI NIPIATSDLG DNWNAELVKA SDVVMANVHP FFAGVSVDLA ASWTWDFWNN
     HNLVLTKGTD KKQIISEVGW PSGGGNDCGD GGNCPNDSAG SVAGIDEMNQ FMSDWVCQAL
     DNGTDYFWFE AFDEPWKIVY NTKNENWEDK WGLMDPARNL KDGLKIPDCG GKTAT
//