Reviewed, UniProtKB/Swiss-Prot Q0CI79 (PMIP_ASPTN)

Names and origin

Protein names

Recommended name:
    Mitochondrial intermediate peptidase
      Short name=MIP
    EC=3.4.24.59
Alternative name(s):
    Octapeptidyl aminopeptidase

Gene names

Name:	oct1
ORF Names:	ATEG_06605

Organism

Aspergillus terreus (strain NIH 2624 / FGSC A1156) [Complete proteome]

Taxonomic identifier

341663 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	802 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cleaves proteins, imported into the mitochondrion, to their mature size. While most mitochondrial precursor proteins are processed to the mature form in one step by mitochondrial processing peptidase (MPP), the sequential cleavage by MIP of an octapeptide after initial processing by MPP is a required step for a subgroup of nuclear-encoded precursor proteins destined for the matrix or the inner membrane By similarity.
Catalytic activity	Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.
Cofactor	Binds 1 zinc ion By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the peptidase M3 family.
Sequence caution	The sequence EAU33149.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence EAU33149.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 24	24	Mitochondrion Potential
	Chain	25 – 802	778	Mitochondrial intermediate peptidase		PRO_0000338574
Sites
	Active site	566	1	By similarity
	Metal binding	565	1	Zinc; catalytic By similarity
	Metal binding	569	1	Zinc; catalytic By similarity
	Metal binding	572	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0CI79-1 [UniParc]. Last modified June 10, 2008. Version 2. Checksum: 3E92F4BA281CEB5F	FASTA	802	89,472
	10 20 30 40 50 60 MGGPKLLMPL RPRPWTCRTC LQRLHSARRS LKTAASPSPQ STSFEYNRSA HNTQKSTTDD 70 80 90 100 110 120 ETLRRVFDSP SFWKEFSQRS SLHTKPTGLV QNQYLTSPEG FRTFANVSLH KCQAIVHKVL 130 140 150 160 170 180 AASTLEEYRD MARDLDRLSD LLCRVIDLSD FIRVIHPDPQ VQEAATQAYA LMFDYMNVLN 190 200 210 220 230 240 TTTGLNDQLK KAAANPDVTS HWSAEEKIVA QILIKDFSNS AIHMPPNERQ RFVNLSNEIS 250 260 270 280 290 300 QLGSNFVNAA EPAKSHVVVG TNSLRGLDPL LVQQIKRWNR TASVPTMGMI PRLVLRSVHD 310 320 330 340 350 360 EGVRKDVYLA TRTSSSRQIK RLEQLLAKRA ELAQLSGYAS FGHMTLSDKM AKSPEAVSNF 370 380 390 400 410 420 LTSLVGSNRQ FVHEELAQLQ GMKGSSPLQA WDHAYYVHQR VMQYSQSRRS RELSIVPEFF 430 440 450 460 470 480 SLGTVMQGLS RLFNRLYGVR LVPQETAPGE TWNPDVRRLD VVDEAERHIA VIYCDLFSRP 490 500 510 520 530 540 NKHPNPAHFT LRCSREISNQ EVAECASMDQ SAHPNDGMAT AVDPQSKTLR QLPTIALVCD 550 560 570 580 590 600 FPDPPATSAG RPSLLSEHSV RTLFHEMGHA LHSILGQTRL QSISGTRCAT DFAELPSVLM 610 620 630 640 650 660 ERFATAPEVL SMYARHWQTD QPLSENMMRS MEQDRTAHGS IYGAVENEAQ ILMALVDQAY 670 680 690 700 710 720 HSVPAEAAGK IDSTAIYHQV SQAHSSLPEP SDTQPPTSWQ GFFGHLYGYG ATYYSYIFDR 730 740 750 760 770 780 AIANKLWEDV FQSGQASVDR NAGERYKNEV LRWGGGRNGW DCVAGVLGSG NPSNADGRLV 790 800 EGGDDAMREV GRWGLGRDGV SG

References

[1]

"Annotation of the Aspergillus terreus NIH2624 genome." Birren B.W., Lander E.S., Galagan J.E., Nusbaum C., Devon K., Henn M., Ma L.-J., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M., Kleber M., Mauceli E.W., Brockman W., Rounsley S., Young S.K., LaButti K. , Pushparaj V., DeCaprio D., Crawford M., Koehrsen M., Engels R., Montgomery P., Pearson M., Howarth C., Larson L., Luoma S., White J., Alvarado L., Kodira C.D., Zeng Q., Oleary S., Yandava C., Denning D.W., Nierman W.C., Milne T., Madden K. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CH476602 Genomic DNA. Translation: EAU33149.1. Sequence problems.
RefSeq	XP_001215783.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	M03.006.
Genome annotation databases
GeneID	4322361.
Family and domain databases
InterPro	IPR001567. Pept_M3A_M3B. IPR006025. Pept_M_Zn_BS. [Graphical view]
Pfam	PF01432. Peptidase_M3. 1 hit. [Graphical view]
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PMIP_ASPTN

Accession

Primary (citable) accession number: Q0CI79

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	June 10, 2008
Last modified:	June 16, 2009
This is version 19 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)