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Protein

Probable rhamnogalacturonase B

Gene

rhgB

Organism
Aspergillus terreus (strain NIH 2624 / FGSC A1156)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Pectinolytic enzymes consist of four classes of enzymes: pectine lyase, polygalacturonase, pectin methylesterase and rhamnogalacturonase. Hydrolyzes alpha-D-galacturonopyranosyl-(1,2)-alpha-L-rhamnopyranosyl linkages in the backbone of the hairy regions of pectins (By similarity).By similarity

Catalytic activityi

Endohydrolysis of alpha-D-GalA-(1->2)-alpha-L-Rha glycosidic bond in the rhamnogalacturonan I backbone with initial inversion of anomeric configuration releasing oligosaccharides with beta-D-GalA at the reducing end.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei218Proton donorBy similarity1
Active sitei293By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cell wall biogenesis/degradation, Polysaccharide degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Probable rhamnogalacturonase B (EC:3.2.1.171)
Short name:
RGase B
Short name:
RHG B
Gene namesi
Name:rhgB
ORF Names:ATEG_07607
OrganismiAspergillus terreus (strain NIH 2624 / FGSC A1156)
Taxonomic identifieri341663 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000007963 Componenti: Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:ATEG_07607.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000039439021 – 526Probable rhamnogalacturonase BAdd BLAST506

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi41 ↔ 67By similarity
Glycosylationi144N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi220 ↔ 237By similarity
Glycosylationi238N-linked (GlcNAc...)Sequence analysis1
Glycosylationi253N-linked (GlcNAc...)Sequence analysis1
Glycosylationi320N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi343 ↔ 349By similarity
Disulfide bondi371 ↔ 380By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliQ0CFC7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 28 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000217527.
OMAiAWALQID.
OrthoDBiEOG092C32CC.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0CFC7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHVNTLSVLS LVGLVPLAAA RLSGSVGPLT SASAKAATKT CNVLDYGAAA
60 70 80 90 100
DKTTDLGAPL ASAFADCKTG GLVYVPPGDY ALESWAQLSG GKAWALQIDG
110 120 130 140 150
TIYRTGTDGG NMIFIEHSSD FELFSSTSSG AMQGLGYEFH KDDNWSGPRL
160 170 180 190 200
LRLYDVSDFA LHDFILVDSP SFHLSLDTCS NGEVYNMAIR GGNHGALDGV
210 220 230 240 250
DVWSTNIWIH DVEVTNKDEC VTVKSPAQNI LVENIYCNWS GGCGMGSLGA
260 270 280 290 300
DTNISDITYR NVYTWNSNQM MLIKSNGGSG TVSNVVLENF IGHGNAYSLD
310 320 330 340 350
IDSAWSSMST QSGDGVEFSN FTIRNWKGTE ANGAERGPVK IICPNGAPCY
360 370 380 390 400
EMYIEDFAMW TEEGDEQWYT CQSAFGSGFC LQSGSDYSSY EATTSTATSA
410 420 430 440 450
PSGYSAPTMA SDLTRDFGST VSIPIPTIPT SFYPGATPYS ALMGAQSSAS
460 470 480 490 500
ANVRAVAFGT SAVVSRAGSS SRPAQTGSFL TKSTTAAAGS IMGPTAAAVQ
510 520
GVCAPPAGQG RGAARYGGHR RHGHRH
Length:526
Mass (Da):55,261
Last modified:October 17, 2006 - v1
Checksum:i1FF3741862EDC215
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH476604 Genomic DNA. Translation: EAU31869.1.
RefSeqiXP_001216228.1. XM_001216228.1.

Genome annotation databases

EnsemblFungiiCADATEAT00001304; CADATEAP00001304; CADATEAG00001304.
GeneIDi4322704.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH476604 Genomic DNA. Translation: EAU31869.1.
RefSeqiXP_001216228.1. XM_001216228.1.

3D structure databases

ProteinModelPortaliQ0CFC7.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADATEAT00001304; CADATEAP00001304; CADATEAG00001304.
GeneIDi4322704.

Organism-specific databases

EuPathDBiFungiDB:ATEG_07607.

Phylogenomic databases

HOGENOMiHOG000217527.
OMAiAWALQID.
OrthoDBiEOG092C32CC.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRHGB_ASPTN
AccessioniPrimary (citable) accession number: Q0CFC7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 18, 2010
Last sequence update: October 17, 2006
Last modified: October 5, 2016
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.