Q0CD12 (KATG_ASPTN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
ORF Names:	ATEG_08422

Organism

Aspergillus terreus (strain NIH 2624 / FGSC A1156) [Complete proteome]

Taxonomic identifier

341663 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	737 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity.
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. 2 H2O2 = O2 + 2 H2O.
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 737	737	Catalase-peroxidase		PRO_0000354103
Sites
	Active site	90	1	Proton acceptor By similarity
	Metal binding	260	1	Iron (heme axial ligand) By similarity
	Site	86	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	89 ↔ 219		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-245) By similarity
	Cross-link	219 ↔ 245		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-89) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0CD12 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 59F5B80D7B3F9425	FASTA	737	81,333
	10 20 30 40 50 60 MGESKCPVNF AGGGTRNKDW WPDQLRLNIL RQHTSASNPL DADFDYAAAF NSLDYNALKK 70 80 90 100 110 120 DLEALMTDSQ DWWPADFGHY GGLFIRMAWH SAGTYRVFDG RGGAGQGQQR FAPLNSWPDN 130 140 150 160 170 180 ASLDKARRLL WPIKQKYGNK ISWADLMILA GNVALESMGF KPFGFSGGRA DTWEADESVY 190 200 210 220 230 240 WGGEKTWFPK GNDVRYPNDD IYTRDLENPL AASHMGLIYV NPEGPNGNPD PKAAARDIRV 250 260 270 280 290 300 TFGRMAMNDE ETVALIAGGH SFGKTHGASS GDHCGPEPEA AGLEAQGLGW QSKYGSGSGR 310 320 330 340 350 360 DAITSGLEVT WTKTPTRWST NFLEYLFAFD WELTKSPAGA NQWVAKNADA IIPDAFDPSK 370 380 390 400 410 420 KHKPQMLTTD LALRYDPAYE KIARRFLENP DQFADAFARA WFKLTHRDMG PRARYVGPEV 430 440 450 460 470 480 PSEVLIWQDP IPAVNHPLVD ASDIASLKQA ILNSGVDRSK FVSTAWAAAS TFRGGDKRGG 490 500 510 520 530 540 ANGARIRLAP QRDWEVNNQP WLKESLAALE KIQSSFNGSR SDRKKISLAD LIVLAGCAAV 550 560 570 580 590 600 ESAAQEAGHA VSVPFTPGRM DASQEETDVE SFSHMEPVAD GFRNYSTAPT RRRAEHYLVD 610 620 630 640 650 660 KAQMLTLSAP EMTALVGGLR ALNANYDGSA HGVFTSRPGY LTNDFFVNLL DMGTTWKPTD 670 680 690 700 710 720 ASGELYEGAD RRTGSKKWTA TRVDLVFGSH AELRAIAEVY GSSDGERKFV KDFVAAWNKV 730 MNLDRFDLKR ENVPARL

References

[1]

"Annotation of the Aspergillus terreus NIH2624 genome." Birren B.W., Lander E.S., Galagan J.E., Nusbaum C., Devon K., Henn M., Ma L.-J., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M., Kleber M., Mauceli E.W., Brockman W., Rounsley S., Young S.K., LaButti K. , Pushparaj V., DeCaprio D., Crawford M., Koehrsen M., Engels R., Montgomery P., Pearson M., Howarth C., Larson L., Luoma S., White J., Alvarado L., Kodira C.D., Zeng Q., Oleary S., Yandava C., Denning D.W., Nierman W.C., Milne T., Madden K. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NIH 2624 / FGSC A1156.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH476605 Genomic DNA. Translation: EAU31595.1.
RefSeq	XP_001217043.1. XM_001217043.1.
3D structure databases
ProteinModelPortal	Q0CD12.
SMR	Q0CD12. Positions 14-728.
ModBase	Search...
Protein family/group databases
PeroxiBase	3413. AteCP01.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADATEAT00005560; CADATEAP00005560; CADATEAG00005560.
GeneID	4353293.
Phylogenomic databases
GeneTree	EFGT00050000003307.
OrthoDB	EOG41CB4B.
Family and domain databases
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_ASPTN

Accession

Primary (citable) accession number: Q0CD12

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	October 17, 2006
Last modified:	October 19, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program