Q0CCA0 (MANBB_ASPTN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable beta-mannosidase B
EC=3.2.1.25

Alternative name(s):
Mannanase B
Short name=Mannase B

Gene names

Name:	mndB
ORF Names:	ATEG_08684

Organism

Aspergillus terreus (strain NIH 2624 / FGSC A1156) [Complete proteome]

Taxonomic identifier

341663 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus › Aspergillus terreus

Protein attributes

Sequence length	843 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of all N-linked glycoprotein oligosaccharides. Involved in the degradation of mannan and galactomannan By similarity.
Catalytic activity	Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides.
Pathway	Glycan metabolism; N-glycan degradation.
Subcellular location	Secreted Probable.
Sequence similarities	Belongs to the glycosyl hydrolase 2 family.
Sequence caution	The sequence EAU30816.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	beta-mannosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – ?		Potential
	Chain	? – 843		Probable beta-mannosidase B		PRO_0000394657
Sites
	Active site	430	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	721	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q0CCA0 [UniParc]. Last modified June 15, 2010. Version 2. Checksum: EE4DB1B4648C2506	FASTA	843	96,245
	10 20 30 40 50 60 MASFFQQSLA TGWSFKDAED NSAEAWMPVA QVPSVVHQDL IANNKLQDPY VGFRELDARW 70 80 90 100 110 120 VNEKSWTYRT VFQKPAVPAG SSVILAFDGL DTFAKVKLNG NVILESNNMF LAHRIDVTKA 130 140 150 160 170 180 LGADGDHVLE IDFDCAMLRA RELRAKDPQH KWVGFNGDPA RMGVRKAQYH WGWDWGPVLM 190 200 210 220 230 240 TAGIWRDVRL EVYTARVADL WTETDLAVDH HAAQISAFAQ VEGAISSSKV NFILSLHGQE 250 260 270 280 290 300 VARAVAEPQD QVAKVAFDVQ QPSLWWPNGY GDPTLYEISA TLDQDGATVH QISKKIGIRT 310 320 330 340 350 360 AEVVQRPDKH GKSFFFRING VDIFCGGSCW IPADNLLPSI SAERYRKWIE LMVHGRQVMI 370 380 390 400 410 420 RVWGGGCYED DSFYQACDEL GVMVWQDFMF GCGNYPTWPE MLESVEKEAI YNVRRLRHHP 430 440 450 460 470 480 SIVVYVGNNE DYQVQEQQGL TYNFEDKDPQ NWLKSDFPAR YIYEKILPEV VQRYSPSTFY 490 500 510 520 530 540 HPGSPWGDGK ITSDPTVGDM HQWNVWHGTQ EKYQIFDTLG GRFNSEFGME AFPHMSTIEY 550 560 570 580 590 600 FVENEKDKYP QSHVLDFHNK ADGHERRIAT YLVENLRTAT DLETYIYLTQ VVQAETMMFG 610 620 630 640 650 660 YRGWRRQWGD ERHCGGALLW QLNDCWPTIS WAIVDYFLRP KPAFYAVARV LNPIAVGVRR 670 680 690 700 710 720 EHHDWSVTHA QPPKKSKFEL WVASNLQKET RGMVELKFLS VDTGREIRER IVREDVIIVP 730 740 750 760 770 780 NGTTDIIVDG VIDHQEYAEP HVLAARLWVD GKIVARDVDW PQPFKYLDLS GRGLEVKTVS 790 800 810 820 830 840 TSDDQQTLLI SAQKPVKCLV FEERDGVRVS DSAMDIVPGD EQTVTITGLK ADAPPLKYKY LGQ

References

[1]

"Annotation of the Aspergillus terreus NIH2624 genome." Birren B.W., Lander E.S., Galagan J.E., Nusbaum C., Devon K., Henn M., Ma L.-J., Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M., Kleber M., Mauceli E.W., Brockman W., Rounsley S., Young S.K., LaButti K. , Pushparaj V., DeCaprio D., Crawford M., Koehrsen M., Engels R., Montgomery P., Pearson M., Howarth C., Larson L., Luoma S., White J., Alvarado L., Kodira C.D., Zeng Q., Oleary S., Yandava C., Denning D.W., Nierman W.C., Milne T., Madden K. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NIH 2624 / FGSC A1156.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH476606 Genomic DNA. Translation: EAU30816.1. Sequence problems.
RefSeq	XP_001217270.1. XM_001217269.1.
3D structure databases
ProteinModelPortal	Q0CCA0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADATEAT00006706; CADATEAP00006706; CADATEAG00006706.
GeneID	4323103.
Phylogenomic databases
eggNOG	COG3250.
HOGENOM	HOG000186861.
OrthoDB	EOG408RH8.
Enzyme and pathway databases
UniPathway	UPA00280.
Family and domain databases
Gene3D	2.60.40.320. 1 hit. 3.20.20.80. 2 hits.
InterPro	IPR008979. Galactose-bd-like. IPR013812. Glyco_hydro_2/20_Ig-like. IPR006102. Glyco_hydro_2_Ig-like. IPR006104. Glyco_hydro_2_N. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00703. Glyco_hydro_2. 1 hit. PF02837. Glyco_hydro_2_N. 1 hit. [Graphical view]
SUPFAM	SSF49785. Gal_bind_like. 1 hit. SSF49303. Glyco_hydro_2Ig. 1 hit. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00719. GLYCOSYL_HYDROL_F2_1. False negative. PS00608. GLYCOSYL_HYDROL_F2_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MANBB_ASPTN

Accession

Primary (citable) accession number: Q0CCA0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 15, 2010
Last sequence update:	June 15, 2010
Last modified:	May 1, 2013
This is version 38 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program