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Q0C661

- BIOB_HYPNA

UniProt

Q0C661 - BIOB_HYPNA

Protein

Biotin synthase

Gene

bioB

Organism
Hyphomonas neptunium (strain ATCC 15444)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (17 Oct 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi58 – 581Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi62 – 621Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi65 – 651Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi102 – 1021Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi133 – 1331Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi193 – 1931Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi265 – 2651Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciHNEP228405:GI69-48-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:HNE_0048
    OrganismiHyphomonas neptunium (strain ATCC 15444)
    Taxonomic identifieri228405 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesHyphomonadaceaeHyphomonas
    ProteomesiUP000001959: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 320320Biotin synthasePRO_0000381431Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi228405.HNE_0048.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0C661.
    SMRiQ0C661. Positions 9-314.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239957.
    KOiK01012.
    OMAiDETQALC.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q0C661-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTSSDLTPRQ NWTREEIAAI YDLPLDQLFA RALAVKAAHW ADGAVQKSQL    50
    LSIKTGGCAE NCGYCSQSAS FKTGLKAEKL LPVEEVVDAA RRAKANGADR 100
    FCMGAAWRSL KDRDLPKVAE MISAVKAEGL ETCVTLGMLE EGQAEALKEA 150
    GLDYYNHNLD TSREYYSTVV TTRTFDDRIE TLGRARQAGI KLCCGGILGM 200
    GESRSDRVGL IHELSLLSPH PESVPVNKLV PIEGTPLANS EELDTLELAR 250
    AIAVCRIVFP KSWVRLSAGR ETMSAEGQAL CILAGANSIF VGDRLLTTAN 300
    PAMDKDARLL ESVGGGCSKC 320
    Length:320
    Mass (Da):34,481
    Last modified:October 17, 2006 - v1
    Checksum:i33668BBD1222A5D4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000158 Genomic DNA. Translation: ABI78784.1.
    RefSeqiYP_758782.1. NC_008358.1.

    Genome annotation databases

    EnsemblBacteriaiABI78784; ABI78784; HNE_0048.
    GeneIDi4289438.
    KEGGihne:HNE_0048.
    PATRICi32212880. VBIHypNep17450_0048.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000158 Genomic DNA. Translation: ABI78784.1 .
    RefSeqi YP_758782.1. NC_008358.1.

    3D structure databases

    ProteinModelPortali Q0C661.
    SMRi Q0C661. Positions 9-314.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 228405.HNE_0048.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABI78784 ; ABI78784 ; HNE_0048 .
    GeneIDi 4289438.
    KEGGi hne:HNE_0048.
    PATRICi 32212880. VBIHypNep17450_0048.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239957.
    KOi K01012.
    OMAi DETQALC.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci HNEP228405:GI69-48-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 15444.

    Entry informationi

    Entry nameiBIOB_HYPNA
    AccessioniPrimary (citable) accession number: Q0C661
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: October 17, 2006
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3