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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Hyphomonas neptunium (strain ATCC 15444)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei51 – 511S-adenosyl-L-methionineUniRule annotation
Binding sitei76 – 761S-adenosyl-L-methionineUniRule annotation
Binding sitei103 – 1031S-adenosyl-L-methionineUniRule annotation
Active sitei125 – 1251By similarity
Binding sitei125 – 1251S-adenosyl-L-methionineUniRule annotation
Binding sitei129 – 1291SubstrateUniRule annotation
Binding sitei161 – 1611SubstrateUniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciHNEP228405:GI69-109-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:HNE_0109
OrganismiHyphomonas neptunium (strain ATCC 15444)
Taxonomic identifieri228405 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesHyphomonadaceaeHyphomonas
ProteomesiUP000001959: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 219219tRNA (guanine-N(7)-)-methyltransferasePRO_0000288159Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi228405.HNE_0109.

Structurei

3D structure databases

ProteinModelPortaliQ0C600.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni199 – 2024Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073969.
KOiK03439.
OMAiIGCEPFV.
OrthoDBiEOG6K6VBC.

Family and domain databases

HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0C600-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRSFGRTGGR PLSPRQKTLM AEKLDTYRIA EGDGPLDLCA MFPGKEAFWL
60 70 80 90 100
EIGFGGAEHM IAQAAQNPDV GIIGCEPFLE GIAKALGGIE ETDLNNVRVW
110 120 130 140 150
PDDARPLIAR MPDQCLDRAF VLFPDPWPKR RQHKRRLVQA DFLSILHPKM
160 170 180 190 200
KPGARLRFAT DVKSYADEAL LTFVSDGRFE WLAASPDDWR CPPQDHVTTR
210
YESKRLGDCK PVWFDFRIR
Length:219
Mass (Da):24,900
Last modified:October 17, 2006 - v1
Checksum:i68CB3009E2F1DF9E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000158 Genomic DNA. Translation: ABI76398.1.
RefSeqiWP_011645143.1. NC_008358.1.
YP_758843.1. NC_008358.1.

Genome annotation databases

EnsemblBacteriaiABI76398; ABI76398; HNE_0109.
GeneIDi4288247.
KEGGihne:HNE_0109.
PATRICi32213002. VBIHypNep17450_0109.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000158 Genomic DNA. Translation: ABI76398.1.
RefSeqiWP_011645143.1. NC_008358.1.
YP_758843.1. NC_008358.1.

3D structure databases

ProteinModelPortaliQ0C600.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi228405.HNE_0109.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABI76398; ABI76398; HNE_0109.
GeneIDi4288247.
KEGGihne:HNE_0109.
PATRICi32213002. VBIHypNep17450_0109.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073969.
KOiK03439.
OMAiIGCEPFV.
OrthoDBiEOG6K6VBC.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciHNEP228405:GI69-109-MONOMER.

Family and domain databases

HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15444.

Entry informationi

Entry nameiTRMB_HYPNA
AccessioniPrimary (citable) accession number: Q0C600
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 17, 2006
Last modified: January 7, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.