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Q0C600 (TRMB_HYPNA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:HNE_0109
OrganismHyphomonas neptunium (strain ATCC 15444) [Complete proteome] [HAMAP]
Taxonomic identifier228405 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesHyphomonadaceaeHyphomonas

Protein attributes

Sequence length219 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 219219tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000288159

Regions

Region199 – 2024Substrate binding By similarity

Sites

Active site1251 By similarity
Binding site511S-adenosyl-L-methionine By similarity
Binding site761S-adenosyl-L-methionine By similarity
Binding site1031S-adenosyl-L-methionine By similarity
Binding site1251S-adenosyl-L-methionine By similarity
Binding site1291Substrate By similarity
Binding site1611Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0C600 [UniParc].

Last modified October 17, 2006. Version 1.
Checksum: 68CB3009E2F1DF9E

FASTA21924,900
        10         20         30         40         50         60 
MRSFGRTGGR PLSPRQKTLM AEKLDTYRIA EGDGPLDLCA MFPGKEAFWL EIGFGGAEHM 

        70         80         90        100        110        120 
IAQAAQNPDV GIIGCEPFLE GIAKALGGIE ETDLNNVRVW PDDARPLIAR MPDQCLDRAF 

       130        140        150        160        170        180 
VLFPDPWPKR RQHKRRLVQA DFLSILHPKM KPGARLRFAT DVKSYADEAL LTFVSDGRFE 

       190        200        210 
WLAASPDDWR CPPQDHVTTR YESKRLGDCK PVWFDFRIR 

« Hide

References

[1]"Comparative genomic evidence for a close relationship between the dimorphic prosthecate bacteria Hyphomonas neptunium and Caulobacter crescentus."
Badger J.H., Hoover T.R., Brun Y.V., Weiner R.M., Laub M.T., Alexandre G., Mrazek J., Ren Q., Paulsen I.T., Nelson K.E., Khouri H.M., Radune D., Sosa J., Dodson R.J., Sullivan S.A., Rosovitz M.J., Madupu R., Brinkac L.M. expand/collapse author list , Durkin A.S., Daugherty S.C., Kothari S.P., Giglio M.G., Zhou L., Haft D.H., Selengut J.D., Davidsen T.M., Yang Q., Zafar N., Ward N.L.
J. Bacteriol. 188:6841-6850(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15444.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000158 Genomic DNA. Translation: ABI76398.1.
RefSeqYP_758843.1. NC_008358.1.

3D structure databases

ProteinModelPortalQ0C600.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING228405.HNE_0109.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABI76398; ABI76398; HNE_0109.
GeneID4288247.
KEGGhne:HNE_0109.
PATRIC32213002. VBIHypNep17450_0109.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073969.
KOK03439.
OMARAFYGRR.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycHNEP228405:GI69-109-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_HYPNA
AccessionPrimary (citable) accession number: Q0C600
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 17, 2006
Last modified: June 11, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways