Q0C4D8 (ISPE_HYPNA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	HNE_0676

Organism

Hyphomonas neptunium (strain ATCC 15444) [Complete proteome] [HAMAP]

Taxonomic identifier

228405 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Hyphomonadaceae › Hyphomonas

Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 298	298	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000335718
Regions
	Nucleotide binding	102 – 112	11	ATP Potential
Sites
	Active site	15	1	By similarity
	Active site	142	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0C4D8 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 21D95D9F90DAC79D	FASTA	298	30,215
	10 20 30 40 50 60 MSTADKSLSG WAPAKVNLYL HVGPPKPNGR HDLESLVMFS GSGAADHLTA EPAEGLSLSV 70 80 90 100 110 120 AGPFAAAAGA GDDNLVLQAA RALQAASGTQ QGARLSLKKW LPVAAGIGGG SSDAATALRL 130 140 150 160 170 180 LTKLWALDPN HAIALAPGLG GDVPVALAGQ PSLMRGEGER VTPLPALPPV YAVLVNPGVP 190 200 210 220 230 240 CPTGPVFRDH DAAGGGAGFA EIDPPPEFSG PKAFAAWLGQ QRNDLESPAI ARVPEIGAVL 250 260 270 280 290 EFLRAQPGVL LARMSGSGAT CFALCEALAF AERATAVIAH DAHKAHWWTA ASRLGAAP

References

[1]

"Comparative genomic evidence for a close relationship between the dimorphic prosthecate bacteria Hyphomonas neptunium and Caulobacter crescentus." Badger J.H., Hoover T.R., Brun Y.V., Weiner R.M., Laub M.T., Alexandre G., Mrazek J., Ren Q., Paulsen I.T., Nelson K.E., Khouri H.M., Radune D., Sosa J., Dodson R.J., Sullivan S.A., Rosovitz M.J., Madupu R., Brinkac L.M. , Durkin A.S., Daugherty S.C., Kothari S.P., Giglio M.G., Zhou L., Haft D.H., Selengut J.D., Davidsen T.M., Yang Q., Zafar N., Ward N.L. J. Bacteriol. 188:6841-6850(2006) [PubMed: 16980487] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15444.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000158 Genomic DNA. Translation: ABI76728.1.
RefSeq	YP_759405.1. NC_008358.1.
3D structure databases
ProteinModelPortal	Q0C4D8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0C4D8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4288168.
GenomeReviews	Gene locus HNE_0676 in contig CP000158_GR.
KEGG	hne:HNE_0676.
NMPDR	fig|228405.5.peg.663.
PATRIC	32214158. VBIHypNep17450_0683.
TIGR	HNE_0676.
Phylogenomic databases
eggNOG	COG1947.
HOGENOM	HBG734593.
OMA	CQLARMS.
PhylomeDB	Q0C4D8.
Enzyme and pathway databases
BioCyc	HNEP81032:HNE_0676-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_HYPNA

Accession

Primary (citable) accession number: Q0C4D8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	October 17, 2006
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program