ID   SAHH_HYPNA              Reviewed;         469 AA.
AC   Q0C427;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   16-JUN-2009, entry version 27.
DE   RecName: Full=Adenosylhomocysteinase;
DE            EC=3.3.1.1;
DE   AltName: Full=S-adenosyl-L-homocysteine hydrolase;
DE            Short=AdoHcyase;
GN   Name=ahcY; OrderedLocusNames=HNE_0788;
OS   Hyphomonas neptunium (strain ATCC 15444).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC   Hyphomonadaceae; Hyphomonas.
OX   NCBI_TaxID=228405;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16980487; DOI=10.1128/JB.00111-06;
RA   Badger J.H., Hoover T.R., Brun Y.V., Weiner R.M., Laub M.T.,
RA   Alexandre G., Mrazek J., Ren Q., Paulsen I.T., Nelson K.E.,
RA   Khouri H.M., Radune D., Sosa J., Dodson R.J., Sullivan S.A.,
RA   Rosovitz M.J., Madupu R., Brinkac L.M., Durkin A.S., Daugherty S.C.,
RA   Kothari S.P., Giglio M.G., Zhou L., Haft D.H., Selengut J.D.,
RA   Davidsen T.M., Yang Q., Zafar N., Ward N.L.;
RT   "Comparative genomic evidence for a close relationship between the
RT   dimorphic prosthecate bacteria Hyphomonas neptunium and Caulobacter
RT   crescentus.";
RL   J. Bacteriol. 188:6841-6850(2006).
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-homocysteine + H(2)O = L-
CC       homocysteine + adenosine.
CC   -!- COFACTOR: Binds 1 NAD per subunit (By similarity).
CC   -!- PATHWAY: Amino-acid biosynthesis; homocysteine biosynthesis; L-
CC       homocysteine from S-adenosyl-L-homocysteine: step 1/1.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the adenosylhomocysteinase family.
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DR   EMBL; CP000158; ABI76706.1; -; Genomic_DNA.
DR   RefSeq; YP_759516.1; -.
DR   GeneID; 4289748; -.
DR   GenomeReviews; CP000158_GR; HNE_0788.
DR   KEGG; hne:HNE_0788; -.
DR   NMPDR; fig|228405.5.peg.770; -.
DR   TIGR; HNE_0788; -.
DR   HOGENOM; Q0C427; -.
DR   OMA; Q0C427; HMRAMKD.
DR   BioCyc; HNEP81032:HNE_0788-MON; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004013; F:adenosylhomocysteinase activity; IEA:HAMAP.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0006730; P:one-carbon compound metabolic process; IEA:HAMAP.
DR   HAMAP; MF_00563; -; 1.
DR   InterPro; IPR000043; Ad_hcy_hydrolase.
DR   InterPro; IPR015878; Ado_hCys_hydrolase_NAD-bd.
DR   Gene3D; G3DSA:3.40.50.1480; Ad_hcy_hydrolase; 1.
DR   PANTHER; PTHR23420; Ad_hcy_hydrolase; 1.
DR   Pfam; PF05221; AdoHcyase; 1.
DR   Pfam; PF00670; AdoHcyase_NAD; 1.
DR   PIRSF; PIRSF001109; Ad_hcy_hydrolase; 1.
DR   TIGRFAMs; TIGR00936; ahcY; 1.
DR   PROSITE; PS00738; ADOHCYASE_1; 1.
DR   PROSITE; PS00739; ADOHCYASE_2; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; Hydrolase; NAD; One-carbon metabolism.
FT   CHAIN         1    469       Adenosylhomocysteinase.
FT                                /FTId=PRO_1000061126.
FT   REGION      222    387       NAD binding (By similarity).
FT   BINDING      60     60       Substrate (By similarity).
FT   BINDING     135    135       Substrate (By similarity).
FT   BINDING     195    195       Substrate (By similarity).
FT   BINDING     225    225       Substrate (By similarity).
FT   BINDING     229    229       Substrate (By similarity).
SQ   SEQUENCE   469 AA;  51572 MW;  48BBCB465036C161 CRC64;
     MSAKAAPQDY FVKDISLAEY GRKEIAIAET EMPGLMAARE EFGPSQPLKG ARICGSLHMT
     IQTAVLIQTL EALGAQVRWV SCNIYSTQDH AAAAIADAGT AVFAYKGETL EEYWDYTDRM
     FQWPDGEGPN LILDDGGDAT MYLILGEKAE SDPSFLEKPT SEEEKYFFAQ IKKRLTASPG
     WFKKTKAGVR GVSEETTTGV NRLYQLEKRG ELPFPAINVN DSVTKSKFDN KYGCKESLVD
     AIRRGTDVMM AGKKAFVAGY GDVGKGSAAS LAGSGARVGV SEVDPICALQ AAMDGFEVLT
     MDEAAPKFDI FVTATGNKDI LTVDHMRAMK DMAIVCNIGH FDNEIQVEGL RNFQWTNIKP
     QVDMITFPDG KRIILLSEGR LVNLGNATGH PSFVMSASFT NQTLAQIELH LRGNEYDNKV
     YTLPKHLDEK VARLHLDKLG VQLTKLSGEQ AAYIGVEQTG PFKPEHYRY
//