Q0C427 (SAHH_HYPNA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenosylhomocysteinase
EC=3.3.1.1

Alternative name(s):
S-adenosyl-L-homocysteine hydrolase
Short name=AdoHcyase

Gene names

Name:	ahcY
Ordered Locus Names:	HNE_0788

Organism

Hyphomonas neptunium (strain ATCC 15444) [Complete proteome] [HAMAP]

Taxonomic identifier

228405 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Hyphomonadaceae › Hyphomonas

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May play a key role in the regulation of the intracellular concentration of adenosylhomocysteine By similarity. HAMAP MF_00563
Catalytic activity	S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine. HAMAP MF_00563
Cofactor	Binds 1 NAD per subunit By similarity. HAMAP MF_00563
Pathway	Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. HAMAP MF_00563
Subcellular location	Cytoplasm By similarity HAMAP MF_00563.
Sequence similarities	Belongs to the adenosylhomocysteinase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	adenosylhomocysteinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 469	469	Adenosylhomocysteinase HAMAP MF_00563		PRO_1000061126
Regions
	Nucleotide binding	196 – 198	3	NAD By similarity
	Nucleotide binding	259 – 264	6	NAD By similarity
	Nucleotide binding	338 – 340	3	NAD By similarity
Sites
	Binding site	60	1	Substrate By similarity
	Binding site	135	1	Substrate By similarity
	Binding site	195	1	Substrate By similarity
	Binding site	225	1	Substrate By similarity
	Binding site	229	1	Substrate By similarity
	Binding site	230	1	NAD By similarity
	Binding site	282	1	NAD By similarity
	Binding site	317	1	NAD By similarity
	Binding site	383	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0C427 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 48BBCB465036C161	FASTA	469	51,572
	10 20 30 40 50 60 MSAKAAPQDY FVKDISLAEY GRKEIAIAET EMPGLMAARE EFGPSQPLKG ARICGSLHMT 70 80 90 100 110 120 IQTAVLIQTL EALGAQVRWV SCNIYSTQDH AAAAIADAGT AVFAYKGETL EEYWDYTDRM 130 140 150 160 170 180 FQWPDGEGPN LILDDGGDAT MYLILGEKAE SDPSFLEKPT SEEEKYFFAQ IKKRLTASPG 190 200 210 220 230 240 WFKKTKAGVR GVSEETTTGV NRLYQLEKRG ELPFPAINVN DSVTKSKFDN KYGCKESLVD 250 260 270 280 290 300 AIRRGTDVMM AGKKAFVAGY GDVGKGSAAS LAGSGARVGV SEVDPICALQ AAMDGFEVLT 310 320 330 340 350 360 MDEAAPKFDI FVTATGNKDI LTVDHMRAMK DMAIVCNIGH FDNEIQVEGL RNFQWTNIKP 370 380 390 400 410 420 QVDMITFPDG KRIILLSEGR LVNLGNATGH PSFVMSASFT NQTLAQIELH LRGNEYDNKV 430 440 450 460 YTLPKHLDEK VARLHLDKLG VQLTKLSGEQ AAYIGVEQTG PFKPEHYRY

References

[1]

"Comparative genomic evidence for a close relationship between the dimorphic prosthecate bacteria Hyphomonas neptunium and Caulobacter crescentus." Badger J.H., Hoover T.R., Brun Y.V., Weiner R.M., Laub M.T., Alexandre G., Mrazek J., Ren Q., Paulsen I.T., Nelson K.E., Khouri H.M., Radune D., Sosa J., Dodson R.J., Sullivan S.A., Rosovitz M.J., Madupu R., Brinkac L.M. , Durkin A.S., Daugherty S.C., Kothari S.P., Giglio M.G., Zhou L., Haft D.H., Selengut J.D., Davidsen T.M., Yang Q., Zafar N., Ward N.L. J. Bacteriol. 188:6841-6850(2006) [PubMed: 16980487] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15444.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000158 Genomic DNA. Translation: ABI76706.1.
RefSeq	YP_759516.1. NC_008358.1.
3D structure databases
ProteinModelPortal	Q0C427.
SMR	Q0C427. Positions 8-469.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0C427.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4289748.
GenomeReviews	Gene locus HNE_0788 in contig CP000158_GR.
KEGG	hne:HNE_0788.
NMPDR	fig|228405.5.peg.770.
PATRIC	32214382. VBIHypNep17450_0794.
TIGR	HNE_0788.
Phylogenomic databases
eggNOG	COG0499.
HOGENOM	HBG352029.
OMA	SAQVWVT.
PhylomeDB	Q0C427.
ProtClustDB	PRK05476.
Enzyme and pathway databases
BioCyc	HNEP81032:HNE_0788-MONOMER.
Family and domain databases
HAMAP	MF_00563. AdoHcyase. [Tree]
InterPro	IPR000043. Adenosylhomocysteinase. IPR015878. Ado_hCys_hydrolase_NAD-bd. IPR020082. S-Ado-L-homoCys_hydrolase_CS. [Graphical view]
KO	K01251.
PANTHER	PTHR23420. Ad_hcy_hydrolase. 1 hit.
Pfam	PF05221. AdoHcyase. 1 hit. PF00670. AdoHcyase_NAD. 1 hit. [Graphical view]
PIRSF	PIRSF001109. Ad_hcy_hydrolase. 1 hit.
SMART	SM00996. AdoHcyase. 1 hit. SM00997. AdoHcyase_NAD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00936. AhcY. 1 hit.
PROSITE	PS00738. ADOHCYASE_1. 1 hit. PS00739. ADOHCYASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAHH_HYPNA

Accession

Primary (citable) accession number: Q0C427

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 17, 2006
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program