Reviewed, UniProtKB/Swiss-Prot Q0C427 (SAHH_HYPNA)

Names and origin

Protein names

Recommended name:
    Adenosylhomocysteinase
    EC=3.3.1.1
Alternative name(s):
    S-adenosyl-L-homocysteine hydrolase
      Short name=AdoHcyase

Gene names

Name:	ahcY
Ordered Locus Names:	HNE_0788

Organism

Hyphomonas neptunium (strain ATCC 15444) [Complete proteome] [HAMAP]

Taxonomic identifier

228405 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Hyphomonadaceae › Hyphomonas

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine. HAMAP MF_00563
Cofactor	Binds 1 NAD per subunit By similarity.
Pathway	Amino-acid biosynthesis; homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. HAMAP MF_00563
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the adenosylhomocysteinase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon compound metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	adenosylhomocysteinase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 469	469	Adenosylhomocysteinase HAMAP MF_00563		PRO_1000061126
Regions
	Region	222 – 387	166	NAD binding By similarity
Sites
	Binding site	60	1	Substrate By similarity
	Binding site	135	1	Substrate By similarity
	Binding site	195	1	Substrate By similarity
	Binding site	225	1	Substrate By similarity
	Binding site	229	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0C427-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 48BBCB465036C161	FASTA	469	51,572
	10 20 30 40 50 60 MSAKAAPQDY FVKDISLAEY GRKEIAIAET EMPGLMAARE EFGPSQPLKG ARICGSLHMT 70 80 90 100 110 120 IQTAVLIQTL EALGAQVRWV SCNIYSTQDH AAAAIADAGT AVFAYKGETL EEYWDYTDRM 130 140 150 160 170 180 FQWPDGEGPN LILDDGGDAT MYLILGEKAE SDPSFLEKPT SEEEKYFFAQ IKKRLTASPG 190 200 210 220 230 240 WFKKTKAGVR GVSEETTTGV NRLYQLEKRG ELPFPAINVN DSVTKSKFDN KYGCKESLVD 250 260 270 280 290 300 AIRRGTDVMM AGKKAFVAGY GDVGKGSAAS LAGSGARVGV SEVDPICALQ AAMDGFEVLT 310 320 330 340 350 360 MDEAAPKFDI FVTATGNKDI LTVDHMRAMK DMAIVCNIGH FDNEIQVEGL RNFQWTNIKP 370 380 390 400 410 420 QVDMITFPDG KRIILLSEGR LVNLGNATGH PSFVMSASFT NQTLAQIELH LRGNEYDNKV 430 440 450 460 YTLPKHLDEK VARLHLDKLG VQLTKLSGEQ AAYIGVEQTG PFKPEHYRY

References

[1]

"Comparative genomic evidence for a close relationship between the dimorphic prosthecate bacteria Hyphomonas neptunium and Caulobacter crescentus." Badger J.H., Hoover T.R., Brun Y.V., Weiner R.M., Laub M.T., Alexandre G., Mrazek J., Ren Q., Paulsen I.T., Nelson K.E., Khouri H.M., Radune D., Sosa J., Dodson R.J., Sullivan S.A., Rosovitz M.J., Madupu R., Brinkac L.M. , Durkin A.S., Daugherty S.C., Kothari S.P., Giglio M.G., Zhou L., Haft D.H., Selengut J.D., Davidsen T.M., Yang Q., Zafar N., Ward N.L. J. Bacteriol. 188:6841-6850(2006) [PubMed: 16980487] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000158 Genomic DNA. Translation: ABI76706.1.
RefSeq	YP_759516.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4289748.
GenomeReviews	Gene locus HNE_0788 in contig CP000158_GR.
KEGG	hne:HNE_0788.
NMPDR	fig|228405.5.peg.770.
TIGR	HNE_0788.
Phylogenomic databases
HOGENOM	Q0C427.
OMA	Q0C427. HMRAMKD.
Enzyme and pathway databases
BioCyc	HNEP81032:HNE_0788-MON.
Family and domain databases
HAMAP	MF_00563. [Tree]
InterPro	IPR000043. Ad_hcy_hydrolase. IPR015878. Ado_hCys_hydrolase_NAD-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.1480. Ad_hcy_hydrolase. 1 hit.
PANTHER	PTHR23420. Ad_hcy_hydrolase. 1 hit.
Pfam	PF05221. AdoHcyase. 1 hit. PF00670. AdoHcyase_NAD. 1 hit. [Graphical view]
PIRSF	PIRSF001109. Ad_hcy_hydrolase. 1 hit.
TIGRFAMs	TIGR00936. ahcY. 1 hit.
PROSITE	PS00738. ADOHCYASE_1. 1 hit. PS00739. ADOHCYASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAHH_HYPNA

Accession

Primary (citable) accession number: Q0C427

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 17, 2006
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)