Q0C1A6 (SYE1_HYPNA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase 1
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase 1
Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	HNE_1783

Organism

Hyphomonas neptunium (strain ATCC 15444) [Complete proteome] [HAMAP]

Taxonomic identifier

228405 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Hyphomonadaceae › Hyphomonas

Protein attributes

Sequence length	486 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 486	486	Glutamate--tRNA ligase 1 HAMAP MF_00022_B		PRO_0000367691
Regions
	Motif	9 – 19	11	"HIGH" region HAMAP MF_00022_B
	Motif	259 – 263	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	262	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0C1A6 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 29EB8DAC026742F2	FASTA	486	53,324
	10 20 30 40 50 60 MSVVTRFAPS PTGMLHIGGA RTALFNYLFA RRNGGRFLLR IEDTDRERST QEATDAILDA 70 80 90 100 110 120 MEWLGLTPDE PPVMQSAQVD RHAAVAHDMV ARGTAFRCYV TPEELQARRD LGEEKRQAAK 130 140 150 160 170 180 QEGISDAEKE ALLAEASQLL APFRSPYRDG ASPPSPDAPF TVRLRAPESG PRTVEDGVQG 190 200 210 220 230 240 TVTIDASEID DLVMLRADGT PTYMLAVVVD DHDMGITHVI RGDDHLRNTF RQVPIYEAMG 250 260 270 280 290 300 WSVPNFSHVP MIHGNDGAKL SKRHGALSTT AYRDMGYLPE AMKAYLLRLG WSHGDQEIFT 310 320 330 340 350 360 DEEAVQVFDV SGINKAPARL DLDKLATVNA HFMRLAADER LFDLICPVLS KNCSLSDAEV 370 380 390 400 410 420 ARIRAALPHM KDRGSTLIEL ANAFAFLYAK RPLELNKNAV KALSDEGKLR LKGLYDDLQR 430 440 450 460 470 480 MSQWSGASIS ETIKSYCAAT GLSMGQIGPP LRAALTGGLP APDLAPVMDW LGREETLARI DDQLAG

References

[1]

"Comparative genomic evidence for a close relationship between the dimorphic prosthecate bacteria Hyphomonas neptunium and Caulobacter crescentus." Badger J.H., Hoover T.R., Brun Y.V., Weiner R.M., Laub M.T., Alexandre G., Mrazek J., Ren Q., Paulsen I.T., Nelson K.E., Khouri H.M., Radune D., Sosa J., Dodson R.J., Sullivan S.A., Rosovitz M.J., Madupu R., Brinkac L.M. , Durkin A.S., Daugherty S.C., Kothari S.P., Giglio M.G., Zhou L., Haft D.H., Selengut J.D., Davidsen T.M., Yang Q., Zafar N., Ward N.L. J. Bacteriol. 188:6841-6850(2006) [PubMed: 16980487] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15444.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000158 Genomic DNA. Translation: ABI77242.1.
RefSeq	YP_760487.1. NC_008358.1.
3D structure databases
ProteinModelPortal	Q0C1A6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0C1A6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4289642.
GenomeReviews	Gene locus HNE_1783 in contig CP000158_GR.
KEGG	hne:HNE_1783.
NMPDR	fig|228405.5.peg.1717.
PATRIC	32216395. VBIHypNep17450_1786.
TIGR	HNE_1783.
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	MAHIPLI.
PhylomeDB	Q0C1A6.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	HNEP81032:HNE_1783-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_HYPNA

Accession

Primary (citable) accession number: Q0C1A6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	October 17, 2006
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program