Q0C0D1 (KATG_HYPNA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	HNE_2115

Organism

Hyphomonas neptunium (strain ATCC 15444) [Complete proteome] [HAMAP]

Taxonomic identifier

228405 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Hyphomonadaceae › Hyphomonas

Protein attributes

Sequence length	723 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Domain	Signal
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Potential
	Chain	30 – 723	694	Catalase-peroxidase HAMAP MF_01961		PRO_0000354809
Sites
	Active site	98	1	Proton acceptor By similarity
	Metal binding	266	1	Iron (heme axial ligand) By similarity
	Site	94	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	97 ↔ 225		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-251) By similarity
	Cross-link	225 ↔ 251		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-97) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0C0D1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: F852D8F2C535EDD9	FASTA	723	79,522
	10 20 30 40 50 60 MDGNDLVENK CPVMHGGITV AGHSNTAWWP ETLNLEILHQ HDTKVSPLGK DFNYRDAVKS 70 80 90 100 110 120 LDFEALKKDM HDLMTNSQDW WPADYGHYGG LMIRLAWHSA GSYRLADGRG GGNTGNIRFA 130 140 150 160 170 180 PLNSWPDNGN LDKARRLLWP IKKKYGNKIS WADLILLSGT IAYESMGLKT YGFSFGREDI 190 200 210 220 230 240 WAPEKDVYWG AEKEWLAPSD ERYTDVDKPE TMENPLAAVQ MGLIYVNPEG VNGKSDPLKS 250 260 270 280 290 300 AAQVRETFAR MAMNDEETAA LTCGGHTVGK AHGNGDAGKL SPPPEGNDLE TQGFGWMNPN 310 320 330 340 350 360 MDGKATNAVT SGIEGAWTTH PTKWDMGYFH LLFGYEWELT KSPAGASQWR PINIKEEDMP 370 380 390 400 410 420 VDPTDPTKRV MPMMTDADMA MKMDPAYNAI CQKFMADQAY FSDTFARAWF KLTHRDLGPR 430 440 450 460 470 480 VRYIGPDAPQ EDLIWQDPVP AGSKSYDVAA VKAKIADSGL SLSEMVSTAW DSARTFRGSD 490 500 510 520 530 540 LRGGANGARI RLAPQKDWAG NEPARLQKVL SVLEPIASAT GASVADVIVL AGNVGVEQAA 550 560 570 580 590 600 KAAGFDISVP FSPGRGDATA EMTDADSFES LEPYADGFRN WQKEDYVVKP EELLLDRAQL 610 620 630 640 650 660 LGLTGPEMTV LLGGMRVMGT NHGGTKHGVF TDKVGALTND FFVNLTDMAN KWVPRGKNAY 670 680 690 700 710 720 DITDRKSGAV KFTATRADLV FGSNSILRAY AEIYAQDDAK EKFVKDFVAA WSKVMDADRF DLA

References

[1]

"Comparative genomic evidence for a close relationship between the dimorphic prosthecate bacteria Hyphomonas neptunium and Caulobacter crescentus." Badger J.H., Hoover T.R., Brun Y.V., Weiner R.M., Laub M.T., Alexandre G., Mrazek J., Ren Q., Paulsen I.T., Nelson K.E., Khouri H.M., Radune D., Sosa J., Dodson R.J., Sullivan S.A., Rosovitz M.J., Madupu R., Brinkac L.M. , Durkin A.S., Daugherty S.C., Kothari S.P., Giglio M.G., Zhou L., Haft D.H., Selengut J.D., Davidsen T.M., Yang Q., Zafar N., Ward N.L. J. Bacteriol. 188:6841-6850(2006) [PubMed: 16980487] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15444.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000158 Genomic DNA. Translation: ABI78396.1.
RefSeq	YP_760812.1. NC_008358.1.
3D structure databases
ProteinModelPortal	Q0C0D1.
SMR	Q0C0D1. Positions 24-722.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0C0D1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4289980.
GenomeReviews	Gene locus HNE_2115 in contig CP000158_GR.
KEGG	hne:HNE_2115.
NMPDR	fig|228405.5.peg.2037.
PATRIC	32217073. VBIHypNep17450_2120.
TIGR	HNE_2115.
Phylogenomic databases
eggNOG	COG0376.
HOGENOM	HBG285610.
OMA	WPNALNL.
PhylomeDB	Q0C0D1.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	HNEP81032:HNE_2115-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_HYPNA

Accession

Primary (citable) accession number: Q0C0D1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	October 17, 2006
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program