Reviewed, UniProtKB/Swiss-Prot Q0BZK3 (HUTI_HYPNA)

Names and origin

Protein names

Recommended name:
    Imidazolonepropionase
    EC=3.5.2.7
Alternative name(s):
    Imidazolone-5-propionate hydrolase

Gene names

Name:	hutI
Ordered Locus Names:	HNE_2395

Organism

Hyphomonas neptunium (strain ATCC 15444) [Complete proteome] [HAMAP]

Taxonomic identifier

228405 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Hyphomonadaceae › Hyphomonas

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+. HAMAP MF_00372
Cofactor	Binds 1 zinc or iron ion per subunit By similarity.
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 3/3. HAMAP MF_00372
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the hutI family.

Ontologies

Keywords
Biological process	Histidine metabolism
Cellular component	Cytoplasm
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	histidine catabolic process to glutamate and formamide Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	imidazolonepropionase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 406	406	Imidazolonepropionase HAMAP MF_00372		PRO_0000306466
Sites
	Metal binding	75	1	Zinc or iron By similarity
	Metal binding	77	1	Zinc or iron By similarity
	Metal binding	245	1	Zinc or iron By similarity
	Metal binding	320	1	Zinc or iron By similarity
	Binding site	84	1	Substrate By similarity
	Binding site	97	1	Substrate By similarity
	Binding site	147	1	Substrate By similarity
	Binding site	180	1	Substrate By similarity
	Binding site	248	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0BZK3-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 7A65FA6FEF7D3823	FASTA	406	43,060
	10 20 30 40 50 60 MQKKRIWTNA RLATMAAGLP GLGIVEDGLI AAEGDRITFA GLASDFPYTE GPSTQGYEVT 70 80 90 100 110 120 DCGGRWILPG LIDCHTHLVW AGSRADEFER RLAGASYEEI ARSGGGIRST VSAVRAASEA 130 140 150 160 170 180 ELVAESLPRL NALIGEGVTT IEIKSGYGLN IEDELKQLRA ARALGEVRPI DVETTLLAAH 190 200 210 220 230 240 TLPPEYEGRA DAYIDLVCNE IIPAAAQAGL ASAVDAFCET IGFTPEQTGR VLSAARHHGL 250 260 270 280 290 300 AVKLHADQLS NLQGGALAAR HNALSADHLE YLDEAGIAAM AQAGMVAVML PGAYYVLRET 310 320 330 340 350 360 HPPPLEGLRR AGVSLAISTD CNPGTSPLTS ILLAMNMGAT LFRMTVEECL LGTTRHAARA 370 380 390 400 LGLEKATGTL EAGKLCNLSI WDIDRPAELV NAMGLNPLHT RVWRGQ

References

[1]

"Comparative genomic evidence for a close relationship between the dimorphic prosthecate bacteria Hyphomonas neptunium and Caulobacter crescentus." Badger J.H., Hoover T.R., Brun Y.V., Weiner R.M., Laub M.T., Alexandre G., Mrazek J., Ren Q., Paulsen I.T., Nelson K.E., Khouri H.M., Radune D., Sosa J., Dodson R.J., Sullivan S.A., Rosovitz M.J., Madupu R., Brinkac L.M. , Durkin A.S., Daugherty S.C., Kothari S.P., Giglio M.G., Zhou L., Haft D.H., Selengut J.D., Davidsen T.M., Yang Q., Zafar N., Ward N.L. J. Bacteriol. 188:6841-6850(2006) [PubMed: 16980487] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000158 Genomic DNA. Translation: ABI76937.1.
RefSeq	YP_761090.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4289897.
GenomeReviews	Gene locus HNE_2395 in contig CP000158_GR.
KEGG	hne:HNE_2395.
NMPDR	fig|228405.5.peg.2309.
TIGR	HNE_2395.
Phylogenomic databases
HOGENOM	Q0BZK3.
OMA	Q0BZK3. MNMACTL.
Enzyme and pathway databases
BioCyc	HNEP81032:HNE_2395-MON.
Family and domain databases
HAMAP	MF_00372. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR005920. HutI. [Graphical view]
Pfam	PF01979. Amidohydro_1. 2 hits. [Graphical view]
ProDom	PD001248. Amidohydro_like. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01224. hutI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HUTI_HYPNA

Accession

Primary (citable) accession number: Q0BZK3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	October 17, 2006
Last modified:	June 16, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)