Q0BZ24 (SYE2_HYPNA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase 2
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase 2
Short name=GluRS 2

Gene names

Name:	gltX2
Ordered Locus Names:	HNE_2579

Organism

Hyphomonas neptunium (strain ATCC 15444) [Complete proteome] [HAMAP]

Taxonomic identifier

228405 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Hyphomonadaceae › Hyphomonas

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 445	445	Glutamate--tRNA ligase 2 HAMAP MF_00022_B		PRO_0000367692
Regions
	Motif	10 – 20	11	"HIGH" region HAMAP MF_00022_B
	Motif	241 – 245	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	244	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0BZ24 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 0B425672130E5C68	FASTA	445	49,179
	10 20 30 40 50 60 MTHPVVRFAP SPTGRLHVGN VRTALINWMF SRGQQGKFIL RIDDTDLERS TAEHEEALKV 70 80 90 100 110 120 DLTWLGLTWD DSFSQSHRFA NYDAAADKLR ALHLLYPCYE TAEELDVKRK IAQTRGRPPV 130 140 150 160 170 180 YDRAALSLTA QDRADLEAKG RKPHWRFKLS GERVEWNDLV RGPQSIDTAS VSDPILIRED 190 200 210 220 230 240 GSYLYTLPSV VDDIEAGITH VVRGEDHVTN SGAQIEIFMA LGGKAPEMAH TPLLIGADGA 250 260 270 280 290 300 ALSKRIGSLS MGELRARGYE PMAICSHLAK LGTSDNIEAR ATLEQLCEEF SFSKIGRAPA 310 320 330 340 350 360 RFDDNDLNAL NAALVHAMPF EAVRERLITL DARAASEPFW LAVRENCTFV ADAISWVDMV 370 380 390 400 410 420 YGSPAPLVAE EDREFISGAA MYLPEGELTT ESWSAWTNAL KAATGRKGRG LFMPLRKALT 430 440 GAEHGPEMSA VLPLIGREKV LQRLS

References

[1]

"Comparative genomic evidence for a close relationship between the dimorphic prosthecate bacteria Hyphomonas neptunium and Caulobacter crescentus." Badger J.H., Hoover T.R., Brun Y.V., Weiner R.M., Laub M.T., Alexandre G., Mrazek J., Ren Q., Paulsen I.T., Nelson K.E., Khouri H.M., Radune D., Sosa J., Dodson R.J., Sullivan S.A., Rosovitz M.J., Madupu R., Brinkac L.M. , Durkin A.S., Daugherty S.C., Kothari S.P., Giglio M.G., Zhou L., Haft D.H., Selengut J.D., Davidsen T.M., Yang Q., Zafar N., Ward N.L. J. Bacteriol. 188:6841-6850(2006) [PubMed: 16980487] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15444.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000158 Genomic DNA. Translation: ABI77356.1.
RefSeq	YP_761269.1. NC_008358.1.
3D structure databases
ProteinModelPortal	Q0BZ24.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0BZ24.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4288956.
GenomeReviews	Gene locus HNE_2579 in contig CP000158_GR.
KEGG	hne:HNE_2579.
NMPDR	fig|228405.5.peg.2478.
PATRIC	32218015. VBIHypNep17450_2587.
TIGR	HNE_2579.
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	LLYPCYE.
ProtClustDB	PRK12558.
Enzyme and pathway databases
BioCyc	HNEP81032:HNE_2579-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE2_HYPNA

Accession

Primary (citable) accession number: Q0BZ24

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	October 17, 2006
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program