Q0BYQ0 (ISPH_HYPNA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
EC=1.17.1.2

Gene names

Name:	ispH
Ordered Locus Names:	HNE_2713

Organism

Hyphomonas neptunium (strain ATCC 15444) [Complete proteome] [HAMAP]

Taxonomic identifier

228405 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Hyphomonadaceae › Hyphomonas

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the IspH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: InterPro
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 315	315	4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191		PRO_1000021133

Sequences

	Sequence		Length	Mass (Da)
	Q0BYQ0 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: BFAADD0772775742	FASTA	315	34,047
	10 20 30 40 50 60 MTTKPPLTLR LAAPRGFCAG VDRAIQIVEE ALKKWGAPVY VRHEIVHNRH VVERLEALGA 70 80 90 100 110 120 IFVEELEECP DDRPVIFSAH GVPKSVPAEA GRRNMIYVDA TCPLVSKVHV EAERHFNAAR 130 140 150 160 170 180 EIVLIGHAGH PEVIGTMGQL PEGSVALIET VEDARAFQPR DHANLAFITQ TTLSVDDTAD 190 200 210 220 230 240 IVAALQSRFP GIATPHKEDI CYATTNRQEA VKVFAPGAEL VLVIGARTSS NSVRLVEVAL 250 260 270 280 290 300 RAGAQDAKLI ASADDIDWSW FNGITTLGLT AGASAPEDLV QGVIEACRDR FEVTVETVKT 310 ADETVTFKLP RVLAG

References

[1]

"Comparative genomic evidence for a close relationship between the dimorphic prosthecate bacteria Hyphomonas neptunium and Caulobacter crescentus." Badger J.H., Hoover T.R., Brun Y.V., Weiner R.M., Laub M.T., Alexandre G., Mrazek J., Ren Q., Paulsen I.T., Nelson K.E., Khouri H.M., Radune D., Sosa J., Dodson R.J., Sullivan S.A., Rosovitz M.J., Madupu R., Brinkac L.M. , Durkin A.S., Daugherty S.C., Kothari S.P., Giglio M.G., Zhou L., Haft D.H., Selengut J.D., Davidsen T.M., Yang Q., Zafar N., Ward N.L. J. Bacteriol. 188:6841-6850(2006) [PubMed: 16980487] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15444.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000158 Genomic DNA. Translation: ABI77892.1.
RefSeq	YP_761393.1. NC_008358.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q0BYQ0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4289089.
GenomeReviews	Gene locus HNE_2713 in contig CP000158_GR.
KEGG	hne:HNE_2713.
NMPDR	fig|228405.5.peg.2600.
PATRIC	32218297. VBIHypNep17450_2718.
TIGR	HNE_2713.
Phylogenomic databases
eggNOG	COG0761.
HOGENOM	HBG335228.
OMA	WGAPVYV.
PhylomeDB	Q0BYQ0.
Enzyme and pathway databases
BioCyc	HNEP81032:HNE_2713-MONOMER.
Family and domain databases
HAMAP	MF_00191. IspH. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
KO	K03527.
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. IspH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH_HYPNA

Accession

Primary (citable) accession number: Q0BYQ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 17, 2006
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program