Reviewed, UniProtKB/Swiss-Prot Q0BYJ6 (TRMFO_HYPNA)

Names and origin

Protein names

Recommended name:
    Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase trmFO
    EC=2.1.1.74
Alternative name(s):
    Folate-dependent tRNA (uracil-5-)-methyltransferase
    Folate-dependent tRNA(M-5-U54)-methyltransferase

Gene names

Name:	trmFO
Ordered Locus Names:	HNE_2768

Organism

Hyphomonas neptunium (strain ATCC 15444) [Complete proteome] [HAMAP]

Taxonomic identifier

228405 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Hyphomonadaceae › Hyphomonas

Protein attributes

Sequence length	467 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH2 = tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD. HAMAP MF_01037
Cofactor	FAD By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the mnmG family. TrmFO subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: HAMAP methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity Inferred from electronic annotation. Source: EC tRNA (uracil-5-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 467	467	Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase trmFO HAMAP MF_01037		PRO_0000346344
Regions
	Nucleotide binding	10 – 15	6	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0BYJ6-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 943568A29D5BC664	FASTA	467	50,980
	10 20 30 40 50 60 MTLKPIHVIG GGMAGSEAAW QIASAGVPVI LHEMRGVRGT DAHQTDKLAE LVCSNSFRSD 70 80 90 100 110 120 DHTTNAVGVI HEEMRRANGL IITTAKDHQV PAGSALAVDR EGFSEAITAK LEAHPLVTIV 130 140 150 160 170 180 REEIAGLPPE DWDSVIVATG PLTSIALAEA VRAHTGETDL AFFDAIAPIV YFESIDMDKA 190 200 210 220 230 240 WRQSRYDKAG PSGDTAAYIN CPMTEEQYNA FLDALLAAPK TEFRDWEKDT PYFEGCLPIE 250 260 270 280 290 300 VMAERGRETL RFGPMKPVGL TNPHNPTVKA HAIVQLRQDN ALGTLWNMVG FQTKLKYAAQ 310 320 330 340 350 360 TDIFRMIPGL EKAEFARLGG IHRNTFLNSP KLLDRQLRMK SMPRLRFAGQ VTGVEGYVES 370 380 390 400 410 420 AAMGLLAGRF AAAERLGQRL EPPPPTTAMG ALISHITGGH LAEKQTFQPM NVNFGLFPDI 430 440 450 460 TDYSKTDENG KRLRGKDKGR AKKIAQAIRA LQDFDAWLAG EAVVAAE

References

[1]

"Comparative genomic evidence for a close relationship between the dimorphic prosthecate bacteria Hyphomonas neptunium and Caulobacter crescentus." Badger J.H., Hoover T.R., Brun Y.V., Weiner R.M., Laub M.T., Alexandre G., Mrazek J., Ren Q., Paulsen I.T., Nelson K.E., Khouri H.M., Radune D., Sosa J., Dodson R.J., Sullivan S.A., Rosovitz M.J., Madupu R., Brinkac L.M. , Durkin A.S., Daugherty S.C., Kothari S.P., Giglio M.G., Zhou L., Haft D.H., Selengut J.D., Davidsen T.M., Yang Q., Zafar N., Ward N.L. J. Bacteriol. 188:6841-6850(2006) [PubMed: 16980487] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000158 Genomic DNA. Translation: ABI75802.1.
RefSeq	YP_761447.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4287632.
GenomeReviews	Gene locus HNE_2768 in contig CP000158_GR.
KEGG	hne:HNE_2768.
NMPDR	fig|228405.5.peg.2654.
TIGR	HNE_2768.
Phylogenomic databases
HOGENOM	Q0BYJ6.
OMA	Q0BYJ6. MKPVGLT.
Enzyme and pathway databases
BioCyc	HNEP81032:HNE_2768-MON.
Family and domain databases
HAMAP	MF_01037. [Tree]
InterPro	IPR004417. Gid. IPR002218. GIDA-rel. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11806. GIDA. 1 hit.
Pfam	PF01134. GIDA. 1 hit. [Graphical view]
ProDom	PD003738. GIDA. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00137. gid_trmFO. 1 hit.
PROSITE	PS01280. GIDA_1. False negative. PS01281. GIDA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRMFO_HYPNA

Accession

Primary (citable) accession number: Q0BYJ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	October 17, 2006
Last modified:	June 16, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)