Reviewed, UniProtKB/Swiss-Prot Q0BY73 (CARA_HYPNA)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	HNE_2892

Organism

Hyphomonas neptunium (strain ATCC 15444) [Complete proteome] [HAMAP]

Taxonomic identifier

228405 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Hyphomonadaceae › Hyphomonas

Protein attributes

Sequence length	386 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 386	386	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_1000138862
Regions
	Domain	198 – 386	189	Glutamine amidotransferase type-1
	Region	1 – 194	194	CPSase HAMAP MF_01209
Sites
	Active site	275	1	Nucleophile By similarity
	Active site	359	1	By similarity
	Active site	361	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0BY73-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: E9FDD63931BF669C	FASTA	386	40,747
	10 20 30 40 50 60 MDSATGALAL EDGTIFAGFG AGAAGIKVGE LCFNTAITGY QEILTDPSYA SQLVLFTFPH 70 80 90 100 110 120 IGNVGANAED HEESTVEAAD AARGAIFREP ITAPSNWRST EDFGVWLARR GIIGLSGIDT 130 140 150 160 170 180 RALTKRIREK GMPKAAICHK PDGKIDFDAL VELARSWNGL DGADLADVVD QDAPFEHTEG 190 200 210 220 230 240 LWQAATGHQT ALANAEFHVA VIDFGVKKNI LRNLASAGAR ATVVNGNVSA EEVLALKPDG 250 260 270 280 290 300 IVFANGPGDP AATFERSGEM IKTLVASGLP ILGICLGHQL LGLALGAKTK KMAQGHHGAN 310 320 330 340 350 360 HPVKDLATGK VEIVSMNHGF TVDPDTLPPG VEESHRSLFD GTNSGLAVKG KPIISVQHHP 370 380 EASPGPQDSF YLFTRFAGLM RDYRAK

References

[1]

"Comparative genomic evidence for a close relationship between the dimorphic prosthecate bacteria Hyphomonas neptunium and Caulobacter crescentus." Badger J.H., Hoover T.R., Brun Y.V., Weiner R.M., Laub M.T., Alexandre G., Mrazek J., Ren Q., Paulsen I.T., Nelson K.E., Khouri H.M., Radune D., Sosa J., Dodson R.J., Sullivan S.A., Rosovitz M.J., Madupu R., Brinkac L.M. , Durkin A.S., Daugherty S.C., Kothari S.P., Giglio M.G., Zhou L., Haft D.H., Selengut J.D., Davidsen T.M., Yang Q., Zafar N., Ward N.L. J. Bacteriol. 188:6841-6850(2006) [PubMed: 16980487] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000158 Genomic DNA. Translation: ABI75793.1.
RefSeq	YP_761570.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q0BY73.
Genome annotation databases
GeneID	4289709.
GenomeReviews	Gene locus HNE_2892 in contig CP000158_GR.
KEGG	hne:HNE_2892.
NMPDR	fig|228405.5.peg.2771.
TIGR	HNE_2892.
Phylogenomic databases
HOGENOM	Q0BY73.
OMA	LFDGSNC.
Enzyme and pathway databases
BioCyc	HNEP81032:HNE_2892-MON.
Family and domain databases
HAMAP	MF_01209. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_HYPNA

Accession

Primary (citable) accession number: Q0BY73

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	October 17, 2006
Last modified:	November 3, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)