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Protein

Hydroxyacylglutathione hydrolase

Gene

gloB

Organism
Hyphomonas neptunium (strain ATCC 15444)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.UniRule annotation

Catalytic activityi

S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per subunit.UniRule annotation

Pathwayi: methylglyoxal degradation

This protein is involved in step 2 of the subpathway that synthesizes (R)-lactate from methylglyoxal.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Hydroxyacylglutathione hydrolase (gloB)
This subpathway is part of the pathway methylglyoxal degradation, which is itself part of Secondary metabolite metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-lactate from methylglyoxal, the pathway methylglyoxal degradation and in Secondary metabolite metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi58Zinc 1UniRule annotation1
Metal bindingi60Zinc 1UniRule annotation1
Metal bindingi62Zinc 2UniRule annotation1
Metal bindingi63Zinc 2UniRule annotation1
Metal bindingi116Zinc 1UniRule annotation1
Metal bindingi135Zinc 1UniRule annotation1
Metal bindingi135Zinc 2UniRule annotation1
Metal bindingi173Zinc 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00619; UER00676.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyacylglutathione hydrolaseUniRule annotation (EC:3.1.2.6UniRule annotation)
Alternative name(s):
Glyoxalase IIUniRule annotation
Short name:
Glx IIUniRule annotation
Gene namesi
Name:gloBUniRule annotation
Ordered Locus Names:HNE_3406
OrganismiHyphomonas neptunium (strain ATCC 15444)
Taxonomic identifieri228405 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesHyphomonadaceaeHyphomonas
Proteomesi
  • UP000001959 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003096511 – 256Hydroxyacylglutathione hydrolaseAdd BLAST256

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi228405.HNE_3406.

Structurei

3D structure databases

ProteinModelPortaliQ0BWR4.
SMRiQ0BWR4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108JMX. Bacteria.
ENOG4111FEZ. LUCA.
HOGENOMiHOG000058041.
KOiK01069.
OMAiHTVGHMI.
OrthoDBiPOG091H03YM.

Family and domain databases

CDDicd07723. hydroxyacylglutathione_hydrola. 1 hit.
Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01374. Glyoxalase_2. 1 hit.
InterProiView protein in InterPro
IPR035680. Clx_II_MBL.
IPR032282. HAGH_C.
IPR017782. Hydroxyacylglutathione_Hdrlase.
IPR001279. Metallo-B-lactamas.
PfamiView protein in Pfam
PF16123. HAGH_C. 1 hit.
PF00753. Lactamase_B. 1 hit.
PIRSFiPIRSF005457. Glx. 1 hit.
SMARTiView protein in SMART
SM00849. Lactamase_B. 1 hit.
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR03413. GSH_gloB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0BWR4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVLIEIHQF PCLNDNYGYL VHEPGSGRTI AIDTPDAAVY LAEAEKMSWT
60 70 80 90 100
ISEIWNTHWH PDHAGGNLKI KEATGCKIIG PAGEADKIPG IDRRVKGGDT
110 120 130 140 150
VELGGATATV IDVPGHTLGH IAFHFPDQEA AFVGDAVFAL GCGRVFEGTM
160 170 180 190 200
EMMWESLKRI KKLPKKTQLY CAHEYTASNA KFAVTIEPEN KALKEYVAWI
210 220 230 240 250
EKRRAEDKPT VPALLERELE TNPFLRADLP EMQLAMGHAG NAAATFGEIR

GRKDRF
Length:256
Mass (Da):28,240
Last modified:October 17, 2006 - v1
Checksum:i605C73768775999A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000158 Genomic DNA. Translation: ABI76184.1.
RefSeqiWP_011648374.1. NC_008358.1.

Genome annotation databases

EnsemblBacteriaiABI76184; ABI76184; HNE_3406.
KEGGihne:HNE_3406.

Similar proteinsi

Entry informationi

Entry nameiGLO2_HYPNA
AccessioniPrimary (citable) accession number: Q0BWR4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: October 17, 2006
Last modified: August 30, 2017
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families