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Q0BWI1 (ASSY_HYPNA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Argininosuccinate synthase

EC=6.3.4.5
Alternative name(s):
Citrulline--aspartate ligase
Gene names
Name:argG
Ordered Locus Names:HNE_3491
OrganismHyphomonas neptunium (strain ATCC 15444) [Complete proteome] [HAMAP]
Taxonomic identifier228405 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesHyphomonadaceaeHyphomonas

Protein attributes

Sequence length413 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP-Rule MF_00005

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP-Rule MF_00005

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00005

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00005.

Sequence similarities

Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Arginine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processarginine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

argininosuccinate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 413413Argininosuccinate synthase HAMAP-Rule MF_00005
PRO_0000263932

Regions

Nucleotide binding14 – 229ATP By similarity

Sites

Binding site411ATP; via amide nitrogen and carbonyl oxygen By similarity
Binding site941Citrulline By similarity
Binding site991Citrulline By similarity
Binding site1241ATP; via amide nitrogen By similarity
Binding site1261Aspartate By similarity
Binding site1301Aspartate By similarity
Binding site1301Citrulline By similarity
Binding site1311Aspartate By similarity
Binding site1341Citrulline By similarity
Binding site1851Citrulline By similarity
Binding site1941Citrulline By similarity
Binding site2701Citrulline By similarity
Binding site2821Citrulline By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0BWI1 [UniParc].

Last modified October 17, 2006. Version 1.
Checksum: 197676852BE6F50B

FASTA41345,656
        10         20         30         40         50         60 
MSNAASAPKK VVLAYSGGLD TSIILKWLQV EFGCEVVTFT ADLGQGEELE PARKKALAAG 

        70         80         90        100        110        120 
VKPENIFIED VREEFVRDFV FPMFRANAVY EGVYLLGTSI ARPLIAKRQI EIADMVGADA 

       130        140        150        160        170        180 
VCHGATGKGN DQVRFELGYY GLNPDIKVIA PWRDWAFKSR TDLLKFAEEH GIEIAKDKRG 

       190        200        210        220        230        240 
EAPFSVDANL LHSSSEGKVL ENPAEPAPEF VHMRTVSPED APDQAEVITI SFEKGDAVAI 

       250        260        270        280        290        300 
NGEAMSPATL LTALNAYGKK HGIGRLDLLE NRFVGMKSRG IYETPGGTIL LVAHRGIEQA 

       310        320        330        340        350        360 
TLDRGAAHLK DELMPKYAEL IYNGFWWSPE REMLQAAIDH SQRWVTGDVT MKLYKGNAYL 

       370        380        390        400        410 
IGRSSPYSLY SEKIVTFEDD HGAYDQKDAA GFIKLNALRL RLLAGRDRKF GKN 

« Hide

References

[1]"Comparative genomic evidence for a close relationship between the dimorphic prosthecate bacteria Hyphomonas neptunium and Caulobacter crescentus."
Badger J.H., Hoover T.R., Brun Y.V., Weiner R.M., Laub M.T., Alexandre G., Mrazek J., Ren Q., Paulsen I.T., Nelson K.E., Khouri H.M., Radune D., Sosa J., Dodson R.J., Sullivan S.A., Rosovitz M.J., Madupu R., Brinkac L.M. expand/collapse author list , Durkin A.S., Daugherty S.C., Kothari S.P., Giglio M.G., Zhou L., Haft D.H., Selengut J.D., Davidsen T.M., Yang Q., Zafar N., Ward N.L.
J. Bacteriol. 188:6841-6850(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15444.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000158 Genomic DNA. Translation: ABI77474.1.
RefSeqYP_762162.1. NC_008358.1.

3D structure databases

ProteinModelPortalQ0BWI1.
SMRQ0BWI1. Positions 10-408.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING228405.HNE_3491.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABI77474; ABI77474; HNE_3491.
GeneID4288392.
KEGGhne:HNE_3491.
PATRIC32219865. VBIHypNep17450_3495.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0137.
HOGENOMHOG000230093.
KOK01940.
OMAGIKSKFD.
OrthoDBEOG6K9QCV.

Enzyme and pathway databases

BioCycHNEP228405:GI69-3492-MONOMER.
UniPathwayUPA00068; UER00113.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPMF_00005. Arg_succ_synth_type1.
InterProIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsTIGR00032. argG. 1 hit.
PROSITEPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASSY_HYPNA
AccessionPrimary (citable) accession number: Q0BWI1
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: October 17, 2006
Last modified: May 14, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways